Electrophoresis Buffer (10×)

Introduction

Tris-Glycine SDS electrophoresis buffer was used in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for protein denaturation. This product is supplied with 10 × concentrate for easy transportation.

Components

Instructions

This product is a 10× concentrate, diluted with Deionized water to 1× working solution before use.

For example: take 100 mL of Electrophoresis Buffer (10×), dilute with Deionized water to 1 L.

1 × Electrophoresis Buffer Components

25 mM Tris, 192 mM Glycine, 0.1% SDS, pH8.3.

Storage

Store at room temperature for 12 months.

Cautions

1. This product is a high concentrate. When the temperature is low, crystals may be precipitated. It can be heated to dissolve. Diluted after it is completely dissolved.

2. Keep the product sealed to prevent from pollution.

3. For your safety and health, please wear the lab coat and disposable gloves before the experiments.