Alternate Names	T-cell surface glycoprotein CD4,CD4,T-cell surface antigen T4/Leu-3,CD4
Swissprot	P06332
References	1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579. 3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Product Details

Form	Liquid
Clone No.	GK1.5(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2b, κ
Isotype Control	FITC Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09842C]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	FITC
	FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD4 Antibody[GK1.5]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
Biotin Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
PE Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
PE Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
APC Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
PerCP Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More

Citations

Frontiers in Immunology (2022) IF: 7.561

Targeting Glutamine Metabolism Ameliorates Autoimmune Hepatitis via Inhibiting T Cell Activation and Differentiation.

DOI: 10.3389/fimmu.2022.880262
	Sample: splenocytes

BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 6.53

Epigallocatechin-3-gallate protects immunity and liver drug-metabolism function in mice loaded with restraint stress

DOI: 10.1016/j.biopha.2020.110418
	Sample: Whole Blood

Marine Drugs (2022) IF: 5.118

In Vitro and In Vivo Dendritic Cell Immune Stimulation Effect of Low Molecular Weight Fucoidan from New Zealand Undaria pinnatifida

DOI: 10.3390/md20030197
PMID: 35323496	Sample: Dendritic cells (DCs)

JOURNAL OF ETHNOPHARMACOLOGY (2023) IF: 5.195

Immunomodulatory effects and mechanisms of Tiepishihu Xiyangshen granules on cyclophosphamide induced immuno-suppression via TLR4/MAPKs and PI3K/AKT/FOXO3a signal pathways

DOI: 10.1016/j.jep.2023.116192
	Sample: T lymphocytes

Food & Function (2021) IF: 5.396

The petroleum ether extract of Brassica rapa L. induces apoptosis of lung adenocarcinoma cells via the mitochondria-dependent pathway

DOI: 10.1039/D1FO01547H
PMID: 34523644	Sample: spleen

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