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FITC Anti-Mouse CD4 Antibody[RM4-5]

Uniprot : P06332
  • Cat.No.:E-AB-F1353C

  • Applications: FCM
  • Isotype: Rat IgG2a, κ
  • Host: Rat

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Antigen Details

Alternate Names T-cell surface glycoprotein CD4,T-cell surface antigen T4/Leu-3,L3T4,T4
Swissprot P06332
Gene ID 12504
References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Product Details

Form Liquid
Clone No. RM4-5(See Other Available Formats)
Host Rat
Isotype Rat IgG2a, κ
Isotype Control FITC Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832C]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation FITC
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
FITC Anti-Mouse CD4 Antibody[RM4-5] 25μg, 100μg Check More

Citations

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