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Free Cholesterol (FC) Colorimetric Assay Kit

Citations(3)

Cat.No.:E-BC-K004-M
Detection instrument: Microplate reader(495-525 nm,optimum wavelength: 510 nm)

Detection method: Colorimetric method

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Detection principle

Free cholesterol produces 4-cholestenone and hydrogen peroxide under the oxidation of cholesterol oxidase. In the presence of 4-aminoamylpyridine and phenol, peroxidase catalyze hydrogen peroxide to form red quinone compounds of benzoquinone imine phenizone. The color depth of the generated quinones is directly proportional to the cholesterol content.

Performance characteristics

Synonyms	FC
Sample type	Serum, plasma, tissue
Sensitivity	0.07 mmol/L
Detection range	0.07-24 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 5.200%Average intra-assay CV: 1.900%
Other instruments required	Micropipettor, Incubator, Vortex mixer, Centrifuge
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), Isopropanol(AR)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.07-24 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human plasma	1
Human serum	1
Rat serum	1
Mouse serum	1
Rabbit serum	1
10% Rat kidney tissue homogenate	1

Note: The diluent is normal saline (0.85% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

AMERICAN JOURNAL OF PATHOLOGY (2022) IF: 5.77

Paraoxonase 1 Ameliorates Renal Lipotoxicity by Activating Lipophagy and Inhibiting Pyroptosis

DOI: 10.1016/j.ajpath.2022.07.011
PMID: 35963464	Sample: Plasma

Vascular Health and Risk Management (2023)

The Effectiveness of Nigella sativa and Ginger as Appetite Suppressants: An Experimental Study on Healthy Wistar Rats

DOI: 10.2147/VHRM.S396295
	Sample: Serum

ANIMAL FEED SCIENCE AND TECHNOLOGY (2021) IF: 3.247

Coating beef tallow with calcium soap improves dietary pellet quality and promotes an effective diet-induced obese mouse model

DOI: 10.1016/j.anifeedsci.2021.115008
	Sample: Plasma

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