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Free Fatty Acids (NEFA/FFA) Fluorometric Assay Kit

Citations(7)

Cat.No.:E-BC-F039
Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/590 nm)

Detection method: Fluorescence method

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Detection principle

Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids.

Performance characteristics

Synonyms	FFA/NEFA
Sample type	Serum, plasma, animal tissue, cells
Sensitivity	1.20 μmol/L
Detection range	1.20-100 μmol/L
Detection method	Fluorescence method
Assay type	Quantitative
Assay time	70 min
Precision	Average inter-assay CV: 5.1%Average intra-assay CV: 4.1%
Other instruments required	Micropipette, Vortex mixer, Centrifuge
Storage	-20℃
Valid period	12 months

Images

The concentration of FFA in rat plasma was significantly increased in T2DM and PPG treated group, and decreased with ALA treatment.

G J Dugbartey et al investigate liver injury induced by diabetes. Free Fatty Acid (FFA) concentration in rat plasma was determined using FFA fluorometric assay kit (E-BC-F039).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.58-20 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	10-20
Rat serum	200-400
Mouse plasma	100-300
Rabbit serum	100-300
10% Rat liver tissue homogenate	200-400
10% Mouse kidney tissue homogenate	200-400
10% Rat brain tissue homogenate	200-400
10% Rat lung tissue homogenate	200-400

Note: The diluent is reagent 1.

Ralated product

Cat Number	Product Name
E-BC-K013-M	Non-esterified Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit
E-BC-K013-S	Non-esterified Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195

Rhamnogalacturonan-I enriched pectin from steamed ginseng ameliorates lipid metabolism in type 2 diabetic rats via gut microbiota and AMPK pathway

DOI: 10.1016/j.jep.2022.115862
PMID: 36283638	Sample: Serum

DRUG AND CHEMICAL TOXICOLOGY (2020) IF: 2.405

Dexamethasone increases renal free fatty acids and xanthine oxidase activity in female rats: could there be any gestational impact?

DOI: 10.1080/01480545.2020.1784190
PMID: 32597234	Sample: Plasma

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) IF: 4.223

Biochemical investigation of human exposure to aflatoxin M1 and its association with risk factors of diabetes mellitus

DOI: 10.1007/s11356-021-14871-w
PMID: 34216342	Sample: Serum

JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2019) IF: 4.49

Association between duration of obesity and severity of ovarian dysfunction in rat-cafeteria diet approach

DOI: 10.1016/j.jnutbio.2019.05.012
	Sample: Serum

Diabetology & Metabolic Syndrome (2022) IF: 5.395

Targeting hepatic sulfane sulfur/hydrogen sulfide signaling pathway with α-lipoic acid to prevent diabetes-induced liver injury via upregulating hepatic CSE/3-MST expression

DOI: 10.1186/s13098-022-00921-x
PMID: 36229864	Sample: Plasma

CELL PROLIFERATION (2022) IF: 8.755

Hypothalamic Hnscr regulates glucose balance by mediating central inflammation and insulin signal

DOI: 10.1111/cpr.13332
	Sample: Liver

Nature Communications (2022) IF: 17.694

Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease

DOI: 10.1038/s41467-022-35481-1
PMID: 36572670	Sample: Serum

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Reviews/Q&A

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Reviews
Q&A

S*****nSubmitted [ Apr 01 2023 ]

Asked: Does this assay work for the quantification of hexanoic acid?

adminSubmitted [ Apr 01 2023 ]

Answered: This kit is used to detect all free fatty acids in sample, it can not detect hexanoic acid content.

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