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Glucose-6-Phosphate Dehydrogenase (G-6-PD) Activity Assay Kit

Citations(1)

Cat.No.:E-BC-K056-M
Detection instrument: Microplate reader(450 nm)

Detection method: Colorimetric method

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96T

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Detection principle

Under the presence of G6PDH, glucose-6-phosphoric acid is oxidized to 6-PG. In this reaction, NADP+ is reduced to NADPH. Under the action of electron coupling reagent 1-MPMS, NADPH reducts wST-8 to form orange formazan, which has the maximum absorption peak at about 450 nm. Formazan generated in the reaction system is proportional to the activity of G6DPH in the sample.

Performance characteristics

Synonyms	G-6-PD, G6PDH
Sample type	Serum, plasma, animal tissue
Sensitivity	0.01 U/L
Detection range	0.01-50 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	20 min
Precision	Average inter-assay CV: 5.800%Average intra-assay CV: 2.100%
Other instruments required	Pipettor, Water bath, Centrifuge
Storage	-20℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-50 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Rat serum	1
Rabbit serum	1
Cynomolgus monkey	1
10% Mouse liver tissue homogenate	3-5
10% Rat kidney tissue homogenate	1
10% Rat spleen tissue homogenate	5-10
10% Mouse brain tissue homogenate	1

Note: The diluent is reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

VETERINARY MICROBIOLOGY (2021) IF: 3.293

Red blood cells serve as a vehicle for PEDV transmission

DOI: 10.1016/j.vetmic.2021.109081
PMID: 33901803	Sample: Blood

Reviews/Q&A

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Reviews
Q&A

P***************rSubmitted [ Jan 08 2022 ]

Asked: what\'s the minimum sample volume?

adminSubmitted [ Jan 08 2022 ]

Answered: The minimum sample volume is 50uL for each hole.

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