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Cat.No.:E-BC-K011-M
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Protocols for metabolism assays
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Synonyms | G6P |
Sample type | Serum, plasma, animal tissue |
Sensitivity | 5.6 μmol /L |
Detection range | 5.6-500 μmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 35 min |
Precision | Average inter-assay CV: 4.300%Average intra-assay CV: 2.100% |
Other instruments required | Pipettor, Water bath, Centrifuge |
Storage | -20℃ |
Valid period | 12 months |
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (5.6-500 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human plasma |
1 |
Mouse serum |
2-3 |
Rat plasma |
1 |
Porcine serum |
1 |
10% Rat spleen tissue homogenate |
2-3 |
10% Rat heart tissue homogenate |
2-3 |
10% Rat liver tissue homogenate |
2-3 |
10% Mouse lung tissue homogenate |
1 |
Note: The diluent is reagent 1.