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Glucose (GLU) Fluorometric Assay Kit

  • Cat.No.:E-BC-F037

  • Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/590 nm)

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  • 96T
  • 48T
Price: $400
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Detection principle

Glucose oxidase can catalyze the oxidation of glucose into gluconic acid and produce hydrogen peroxide. In the presence of peroxidase, hydrogen peroxide reacts with the non-fluorescent substance to form fluorescent substance. The glucose content can be calculated indirectly by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.

Performance characteristics

Synonyms GLU
Sample type Serum, plasma, urine、saliva、milk、cell
Sensitivity 0.1 μmol/L
Detection range 0.1-20 μmol/L
Detection method Fluorescence method
Assay type Quantitative
Assay time 25 min
Precision Average inter-assay CV: 2.800%Average intra-assay CV: 1.700%
Other instruments required Pipettor, Water bath, Centrifuge
Storage -20℃
Valid period 12 months

Images

Blood glucose level was significantly increased in STZ group (***P<0.001) and restored in treated group (**P<0.01).

J Liu et al investigate the function of circ-ITCH for improving renal inflammation and fibrosis in streptozotocin-induced mice. Glucose (GLU) concentration in mouse blood was determined using glucose fluorometric assay kit (E-BC-F037).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.1-20 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human plasma

300-600

Human serum

300-600

Chicken serum

600-1000

Human urine

1

Human milk

400-600

saliva

3-5

Note: The diluent is reagent 1.

Citations

  1. INFLAMMATION RESEARCH (2021) IF: 4.575
    CircRNA circ-ITCH improves renal inflammation and fibrosis in streptozotocin-induced diabetic mice by regulating the miR-33a-5p/SIRT6 axis

    DOI: 10.1007/s00011-021-01485-8

    PMID: 34216220

    Sample: Serum
  2. MICROCHIMICA ACTA (2019) IF: 5.479
    A gold electrode modified with a gold-graphene oxide nanocomposite for non-enzymatic sensing of glucose at near-neutral pH values

    DOI: 10.1007/s00604-019-3796-8

    Sample: Sweat
  3. PHARMACOLOGICAL RESEARCH (2022) IF: 10.334
    Sodium-Glucose Cotransporter-2 (SGLT2) expression in diabetic and non-diabetic failing human cardiomyocytes

    DOI: 10.1016/j.phrs.2022.106448

    PMID: 36096423

    Sample: Ac16 Cell
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