Click here to view cell apoptosis assay products
×

Current Position:Home>>Kits>>Metabolism Assays>> Glutathione Peroxidase (GSH-Px) Activity Assay Kit

Glutathione Peroxidase (GSH-Px) Activity Assay Kit

Citations(54)

  • Cat.No.:E-BC-K096-M

  • Detection instrument: Microplate reader(400-420 nm,optimum wavelength: 412 nm)

Manual MSDS

To Purchase E-BC-K096-M

Size:
  • 96T
  • 48T
Price: $260
Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

 Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.

Performance characteristics

Synonyms GSH-PX
Sample type Serum,plasma,cells,cell culture supernatant,tissue
Sensitivity 34.34 U
Detection range 34.34-1036.64 U
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 40 min
Precision Average inter-assay CV: 8.7%Average intra-assay CV: 2.4%
Other instruments required Micropipettor, Incubator, Vortex mixer, Centrifuge
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Images

The activity of GPx was significantly reduced in the serum of patients with Keshan disease. (**P<0.01)

JC Huang et al investigate the relationship between selenium-related gene ALAD and Keshan disease. Glutathione peroxidase (GSH-Px) activity of human serum was determined using GSH-Px activity assay kit (E-BC-K096-M).

Dilution of sample

The optimal sampling volume are different for different species, also are different for different sample type. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the inhibition ratio is 10%~50% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Mouse serum

3-5

Rat serum

5-8

10% Mouse brain tissue homogenate

1

10% Rat liver tissue homogenate

30-60

HepG2 cell homogenate (5 mgprot/mL)

1

10% Plant tissue homogenate

1-5

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

  1. Frontiers in Pharmacology (2022) IF: 5.988
    ELA-11 protects the heart against oxidative stress injury induced apoptosis through ERK/MAPK and PI3K/AKT signaling pathways

    DOI: 10.3389/fphar.2022.873614

    PMID: 36160397

    		 Sample: Primary Cardiomyocyte
  2. FOOD AND CHEMICAL TOXICOLOGY (2021) IF: 6.025
    Bacillus amyloliquefaciens B10 can alleviate liver apoptosis and oxidative stress induced by aflatoxin B1

    DOI: 10.1016/j.fct.2021.112124

    PMID: 33727180

    		 Sample: Tissue Homogenate
  3. Frontiers in Molecular Biosciences (2022) IF: 6.113
    Biochemical profiling of metabolomics in heavy metal-intoxicated impaired metabolism and its amelioration using plant-based bioactive compound

    DOI: 10.3389/fmolb.2022.1029729

    PMID: 36330218

    		 Sample: Liver
  4. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Comparison of Selenium Nanoparticles and Sodium Selenite on the Alleviation of Early Atherosclerosis by Inhibiting Endothelial Dysfunction and Inflammation in Apolipoprotein E-Deficient Mice

    DOI: 10.3390/ijms222111612

    PMID: 34769040

    		 Sample: Serum
  5. FOOD AND CHEMICAL TOXICOLOGY (2022) IF: 6.025
    Procyanidins inhibit zearalenone-induced apoptosis and oxidative stress of porcine testis cells through activation of Nrf2 signaling pathway

    DOI: 10.1016/j.fct.2022.113061

    PMID: 35489465

    		 Sample: Pig Testicular Cell (St Cell)
  6. Heliyon (2021)
    Treatment with acetate during late pregnancy protects dams against testosterone-induced renal dysfunction

    DOI: 10.1016/j.heliyon.2021.e05920
    Sample: Renal
  7. Journal of Evidence-Based Integrative Medicine (2019)
    Methanol-Extract/Fractions of Dacryodes edulis Leaves Ameliorate Hyperglycemia and Associated Oxidative Stress in Streptozotocin-Induced Diabetic Wistar Rats:

    DOI: 10.1177/2515690X19843832
  8. Toxicology Reports (2023)
    Interactions of alcohol and combination antiretroviral (cART) drug in diabetic male Sprague Dawley rats: Hippocampal perturbations and toxicosis

    DOI: 10.1016/j.toxrep.2023.01.009
    Sample: Brain
  9. Toxicology Reports (2017)
    Diclofenac – induced hepatotoxicity: Low dose of omega-3 fatty acids have more protective effects

    DOI: 10.1016/j.toxrep.2017.12.002
    Sample: Plasma
  10. JOURNAL OF FOOD BIOCHEMISTRY (2020) IF: 1.662
    The effects of chrysin on lipopolysaccharide‐induced sepsis in rats

    DOI: 10.1111/jfbc.13359
    Sample: Lung,Liver,Kidney
  11. MOLECULAR VISION (2017) IF: 2.057
    Pinus densiflora bark extract prevents selenite-induced cataract formation in the lens of Sprague Dawley rat pups

    PMID: 28943754

    		 Sample: Lens Tissue
  12. International Journal of General Medicine (2023) IF: 2.145
    Hematological, Inflammatory, Coagulation, and Oxidative/Antioxidant Biomarkers as Predictors for Severity and Mortality in COVID-19: A Prospective Cohort-Study

    DOI: 10.2147/IJGM.S402206
    Sample: Serum
  13. MOLECULAR VISION (2019) IF: 2.174
    Polyphenol-enriched fraction of Vaccinium uliginosum L. protects selenite-induced cataract formation in the lens of Sprague-Dawley rat pups

    PMID: 30820147

    		 Sample: Lens Tissue
  14. CRYOBIOLOGY (2020) IF: 2.287
    Quercetin supplemented casein-based extender improves the post-thaw quality of rooster semen

    DOI: 10.1016/j.cryobiol.2020.04.010

    PMID: 32437678

    		 Sample: Sperm
  15. BIOMETALS (2020) IF: 2.479
    Cardio-protective effects of a dioxidovanadium(V) complex in male sprague–dawley rats with streptozotocin-induced diabetes

    DOI: 10.1007/s10534-020-00270-0
    Sample: Tissue Homogenate(Heart)
  16. Dose-Response (2022) IF: 2.623
    Pharmacological Role of Capparis decidua (Forssk.) Edgew in Preventing Cyclophosphamide-induced Myelosuppression and Modulating Innate and Adaptive Immune Response:

    DOI: 10.1177/15593258221123672
    Sample: Serum
  17. Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2022) IF: 2.693
    Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains

    DOI: 10.1002/jez.2603
    Sample: Cortex
  18. JOURNAL OF FOOD BIOCHEMISTRY (2021) IF: 2.72
    Antioxidant and antithrombotic effects of green mussels (Perna canaliculus) in rats

    DOI: 10.1111/jfbc.13865

    PMID: 34263474

    		 Sample: Liver，Kidney
  19. JOURNAL OF FOOD BIOCHEMISTRY (2021) IF: 2.72
    A standardized black pepper seed extract containing β-caryophyllene improves cognitive function in scopolamine-induced amnesia model mice via regulation of brain-derived neurotrophic factor and MAPK proteins

    DOI: 10.1111/jfbc.13994

    PMID: 34778972

    		 Sample: Brain
  20. Journal of Diabetes Research (2018) IF: 2.885
    The Effects of Momordica balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers

    DOI: 10.1155/2018/7341242
    Sample: Kidney Tissues
  21. HUMAN & EXPERIMENTAL TOXICOLOGY (2021) IF: 2.903
    Scutellarin alleviates type 2 diabetes (HFD/low dose STZ)-induced cardiac injury through modulation of oxidative stress, inflammation, apoptosis and fibrosis in mice:

    DOI: 10.1177/09603271211045948
    Sample: Heart
  22. THERIOGENOLOGY (2023) IF: 2.923
    Seasonal variations in the metabolomic profile of the ovarian follicle components in Italian Mediterranean Buffaloes

    DOI: 10.1016/j.theriogenology.2023.02.022
    Sample: Follicular Fluid
  23. JOURNAL OF BIOENERGETICS AND BIOMEMBRANES (2021) IF: 2.945
    FoxO4 negatively modulates USP10 transcription to aggravate the apoptosis and oxidative stress of hypoxia/reoxygenation-induced cardiomyocytes by regulating the Hippo/YAP pathway

    DOI: 10.1007/s10863-021-09910-7

    PMID: 34251583

    		 Sample: H9C2 Cells
  24. CURRENT ISSUES IN MOLECULAR BIOLOGY (2023) IF: 2.976
    Chemopreventive Effects of Onosma mutabilis against Azoxymethane-Induced Colon Cancer in Rats via Amendment of Bax/Bcl-2 and NF-κB Signaling Pathways

    DOI: 10.3390/cimb45020057
    Sample: Colonic Tissue
  25. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2022) IF: 3.094
    Native SodB Overexpression of Synechocystis sp. PCC 6803 Improves Cell Growth Under Alcohol Stresses Whereas Its Gpx2 Overexpression Impacts on Growth Recovery from Alcohol Stressors

    DOI: 10.1007/s12010-022-04061-w

    PMID: 35819692

    		 Sample: Pcc 6803 Strains
  26. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2022) IF: 3.168
    ViphyllinTM, a Standardized Black Pepper Extract Exerts Antihyperglycemic Effect and Improves Sciatic Nerve Conduction in High Fat Diet/Streptozotocin-Induced Diabetic Model Rats

    DOI: 10.2147/DMSO.S366609
    Sample: Sciatic Nerve Tissue
  27. Bioengineered (2022) IF: 3.269
    Paeoniflorin alleviates NG-nitro-L-arginine methyl ester (L-NAME)-induced gestational hypertension and upregulates silent information regulator 2 related enzyme 1 (SIRT1) to reduce H2O2-induced endothelial cell damage

    DOI: 10.1080/21655979.2021.2024325

    PMID: 35030965

    		 Sample: Placental Tissue
  28. Biomed Research International (2021) IF: 3.411
    Antioxidant Activity Derived from Marine Green-Lipped Mussel Perna canaliculus Extracts in Mice

    DOI: 10.1155/2021/1622270

    PMID: 34409099

    		 Sample: Liver,Kidney,Cardiac
  29. Hormones-International Journal of Endocrinology and Metabolism (2022) IF: 3.419
    High chitotriosidase and AGE levels in acromegaly: a case-control study

    DOI: 10.1007/s42000-022-00409-3

    PMID: 36241955

    		 Sample: Serum
  30. Frontiers in Behavioral Neuroscience (2022) IF: 3.558
    Acupuncture Relieves Stress-Induced Depressive Behavior by Reducing Oxidative Stress and Neuroapoptosis in Rats

    DOI: 10.3389/fnbeh.2021.783056

    PMID: 35058758

    		 Sample: Hippocampus Tissue
  31. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2022) IF: 3.639
    Flaxseed oil ameliorates aging in D-galactose induced rats via altering gut microbiota and mitigating oxidative damage

    DOI: 10.1002/jsfa.12010

    PMID: 35567370

    		 Sample: Serum
  32. LIFE SCIENCES (2020) IF: 3.647
    Quercetin attenuated oxidative DNA damage through NRF2 signaling pathway in rats with DMH induced colon carcinogenesis

    DOI: 10.1016/j.lfs.2020.117584

    PMID: 32220623

    		 Sample: Tissue Homogenate
  33. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 3.654
    Viphyllin™, a standardized extract from black pepper seeds, mitigates intestinal inflammation, oxidative stress, and anxiety-like behavior in DSS-induced colitis mice

    DOI: 10.1111/jfbc.14306

    PMID: 35766031

    		 Sample: Brain Tissue
  34. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 3.654
    Twelve weeks of treadmill exercise training with green tea extract reduces myocardial oxidative stress and alleviates cardiomyocyte apoptosis in aging rat: The emerging role of BNIP3 and HIF-1α/IGFBP3 pathway

    DOI: 10.1111/jfbc.14397

    PMID: 36069470

    		 Sample: Heart Tissue
  35. Annals of Translational Medicine (2022) IF: 3.932
    Levosimendan protects from sepsis-inducing cardiac dysfunction by suppressing inflammation, oxidative stress and regulating cardiac mitophagy via the PINK-1-Parkin pathway in mice

    DOI: 10.21037/atm-22-483

    PMID: 35280364

    		 Sample: Myocardium
  36. INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 3.943
    Klotho ameliorates the onset and progression of cataract via suppressing oxidative stress and inflammation in the lens in streptozotocin-induced diabetic rats

    DOI: 10.1016/j.intimp.2020.106582

    PMID: 32438076

    		 Sample: Tissue Homogenate
  37. Scientific Reports (2020) IF: 3.998
    Quercetin liposomes ameliorate streptozotocin-induced diabetic nephropathy in diabetic rats

    DOI: 10.1038/s41598-020-59411-7
    Sample: Kidney Tissue
  38. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.052
    Evaluation of testicular torsion management in Ogbomoso, South-Western Nigeria and surgical detorsion-augmented treatment with phytochemical fractions of Corchorus olitorius leaf in experimental rats

    DOI: 10.1016/j.sjbs.2022.103495
    Sample: Testicular Tissue
  39. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Sub-chronic oral toxicity evaluation of herbo-metallic formulation Arshakuthar rasa in rats

    DOI: 10.1016/j.jep.2022.115306

    PMID: 35443217

    		 Sample: Serum
  40. MOLECULAR IMMUNOLOGY (2021) IF: 4.407
    LncRNA NKILA relieves astrocyte inflammation and neuronal oxidative stress after cerebral ischemia/reperfusion by inhibiting the NF-κB pathway

    DOI: 10.1016/j.molimm.2021.08.002

    PMID: 34454183

    		 Sample: Brain Tissue,Ht-22 Cell
  41. MOLECULES (2021) IF: 4.412
    Insight into Seasonal Change of Phytochemicals, Antioxidant, and Anti-Aging Activities of Root Bark of Paeonia suffruticosa (Cortex Moutan) Combined with Multivariate Statistical Analysis

    DOI: 10.3390/molecules26206102

    PMID: 34684685

    		 Sample: Hdf Cell
  42. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Amelioration of risk factors associated with diabetic nephropathy in diet-induced pre-diabetic rats by an uracil-derived diimine ruthenium(II) compound

    DOI: 10.1016/j.biopha.2020.110483

    PMID: 32768965

    		 Sample: Plasma,Tissue Homogenate
  43. Nutrients (2020) IF: 4.546
    Effect of Defatted Dabai Pulp Extract in Urine Metabolomics of Hypercholesterolemic Rats

    DOI: 10.3390/nu12113511

    PMID: 33202660

    		 Sample: Serum
  44. PROCESS BIOCHEMISTRY (2022) IF: 4.885
    Vernodalin regulated the NF-κβp65 signaling in inflammation of lipopolysaccharide -induced sepsis rats

    DOI: 10.1016/j.procbio.2022.10.004
    Sample: Liver,Kidney,Lungs
  45. PROCESS BIOCHEMISTRY (2022) IF: 4.885
    Optimization of ultrasound-assisted extraction, structural, functional, and antioxidant properties of Glycyrrhiza uralensis seed protein

    DOI: 10.1016/j.procbio.2022.11.001
    Sample: Serum,Liver
  46. Antioxidants (2020) IF: 5.014
    Dietary Postbiotic Lactobacillus plantarum Improves Serum and Ruminal Antioxidant Activity and Upregulates Hepatic Antioxidant Enzymes and Ruminal Barrier Function in Post-Weaning Lambs

    DOI: 10.3390/antiox9030250
    Sample: Serum,Ruminal Fluid
  47. Journal of Environmental Sciences (2022) IF: 5.565
    Alleviative effects of a novel strain Bacillus coagulans XY2 on copper-induced toxicity in zebrafish larvae

    DOI: 10.1016/j.jes.2022.02.010

    PMID: 36375957

    		 Sample: Larvae
  48. Frontiers in Pharmacology (2022) IF: 5.988
    Mailuoshutong pill for varicocele-associated male infertility-Phytochemical characterisation and multitarget mechanism.

    DOI: 10.3389/fphar.2022.961011
    Sample: Testicular Tissue
  49. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    Preventing the onset of diabetes-induced chronic kidney disease during prediabetes: The effects of oleanolic acid on selected markers of chronic kidney disease in a diet-induced prediabetic rat model

    DOI: 10.1016/j.biopha.2021.111570

    PMID: 33932738

    		 Sample: Tissue Homogenate(Kidney)
  50. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    D-ribose-L-cysteine prevents oxidative stress and cardiometabolic syndrome in high fructose high fat diet fed rats

    DOI: 10.1016/j.biopha.2021.112017

    PMID: 34399203

    		 Sample: Heart
  51. Nutrients (2022) IF: 6.706
    Cornuside Is a Potential Agent against Alzheimer’s Disease via Orchestration of Reactive Astrocytes

    DOI: 10.3390/nu14153179
    Sample: Hippocampus,Cortex
  52. Nutrients (2022) IF: 6.706
    Ascophyllum nodosum and Fucus vesiculosus Extracts Improved Lipid Metabolism and Inflammation in High-Energy Diet–Induced Hyperlipidemia Rats

    DOI: 10.3390/nu14214665
    Sample: Serum
  53. Antioxidants (2022) IF: 7.675
    Selenoprotein F Knockout Caused Glucose Metabolism Disorder in Young Mice by Disrupting Redox Homeostasis

    DOI: 10.3390/antiox11112105

    PMID: 36358477

    		 Sample: Liver,Serum
  54. FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 8.101
    Integrative analysis of multiomics data identifies selenium-related gene ALAD associating with keshan disease

    DOI: 10.1016/j.freeradbiomed.2022.11.014
    Sample: Serum
Show AllShorten

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
Q

Q***ySubmitted [ Oct 08 2024 ]

Asked: Hello. Is the formula in this manual correct? I cannot get sensible numbers after I input my OD values in your supposed formula. I tried to study the example, hoping to understand the formula. But I also cannot derive the numbers in your appendix II Example analysis. Where did those two 5 come from? Thank you.

Reply

A

adminSubmitted [ Oct 08 2024 ]

Answered: Dear customer, Thanks for your inquiry. For your request of Glutathione Peroxidase (GSH-Px) Activity Assay Kit. The volume of the serum sample added to the reaction system (V) was 20 μL= 0.02 mL, and V=0.02mL was substituted into the formula, (0.23+V) /(0.03+V)=0.25/0.05=5, 0.1/0.02=5. The two 5 in the appendix II Example analysis are calculated by substituting V into the formula. Please feel free to contact us if any questions we could help. Thank you.

Q

M******************OSubmitted [ Oct 26 2022 ]

Asked: My i know is the formula calculation for protein is correct for Cat.No.:E-BC-K096-M ?

Reply

A

adminSubmitted [ Oct 26 2022 ]

Answered: Hi MUHAMMAD, I'll reply you by mail sincerely Lizzy

Q

d*********aSubmitted [ Sep 26 2022 ]

Asked: Is the kit suitable for erythrocyte lysate?

Reply

A

adminSubmitted [ Sep 26 2022 ]

Answered: Dear Dragosalava, Thanks for your inquiry, we confirm with you that product #E-BC-K096-M, Glutathione Peroxidase (GSH-Px) Activity Assay Kit is suitable for erythrocyte lysate.

Q

M*****aSubmitted [ Jul 26 2022 ]

Asked: Is the kit suitable for rabbit sample?

Reply

A

adminSubmitted [ Jul 26 2022 ]

Answered: Yes.

Q

M*****aSubmitted [ Jul 26 2022 ]

Asked: Is the kit suitable for rabbit sample?

Reply

A

adminSubmitted [ Jul 26 2022 ]

Answered: Dear Miss.na,thanks for your message.This kit can be used with rabbit sample. Warm regards,

Q

M*****aSubmitted [ Jul 26 2022 ]

Asked: How to determine the dilution factor of the sample?

Reply

A

adminSubmitted [ Jul 26 2022 ]

Answered: There is no need to dilute the sample, when the Inhibition ratio of sample is 10-50%. If the inhibition ratio is above 50%, the sample needs to be diluted.

A

adminSubmitted [ Jul 26 2022 ]

Answered: There is no need to dilute the sample, when the Inhibition ratio of sample is 10-50%. If the inhibition ratio is above 50%, the sample needs to be diluted.

... Show All Show Less

People Also Bought

About Us

Company Information

Distributors

Contact us

News

Return and Refund Policy

dhl Fedex
Terms and Conditions | Privacy Policy | Cookie Policy  ©2009-2024Elabscience Bionovation Inc. All Rights Reserved.
鄂ICP备18003807号-3
We use cookies to improve your browsing experience, serve personalized ads or content, and for analytics and metrics about our visitors both on this website and other media. By clicking "Accept All", you consent to our use of cookies. To find out more about the cookies we use, see our Cookie Policy
Apply for FREE TRIAL Fluorometric Assay kit
*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?

*Captcha:
Click to load Captcha