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Glycogen Fluorometric Assay Kit

Cat.No.:E-BC-F040
Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm)

Detection method: Fluorescence method

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96T

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Detection principle

Glycogen produces glucose under the action of starch glycosidase, and glucose is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of the peroxidase, hydrogen peroxide be oxidized to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 587 nm is proportional to the glycogen content.

Performance characteristics

Sample type	Animal liver and muscle tissue
Sensitivity	0.06 μg/mL
Detection range	0.06-4.0 μg/mL
Detection method	Fluorescence method
Assay type	Quantitative
Assay time	50min
Precision	Average inter-assay CV: 6.600%Average intra-assay CV: 3.400%
Other instruments required	Micropipettor, Incubator, Centrifuge
Storage	-20℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.06-4.0 μg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Rat liver tissue homogenate	3000-5000
10% Mouse muscle tissue homogenate	10-20

Note: The diluent is reagent 1. Liver samples can be diluted step by step.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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