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Human CRP(C-Reactive Protein) ELISA Kit

Citations(23)Uniprot : P02741

Cat.No.:E-EL-H0043
Reactivity: Human

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96T
48T
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96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T/24
Assay time	3.5h
Detection range	0.39-25 ng/mL
Sensitivity	0.23 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human CRP in samples. No significant cross-reactivity or interference between Human CRP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human CRP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CRP. You can calculate the concentration of Human CRP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 12 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CRP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CRP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	2.50	4.90	16.80	2.50	4.70	18.20
Standard deviation	0.10	0.20	0.60	0.20	0.30	0.70
CV (%)	4.00	4.08	3.57	8.00	6.38	3.85

Recovery

The recovery of Human CRP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-102	95
EDTA plasma (n=8)	85-97	90
Cell culture media (n=8)	88-103	94

Linearity

Samples were spiked with high concentrations of Human CRP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P02741
Synonyms	PTX1, Pentraxin 1
Research Area	Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Biomedicines (2021) IF: 6.081

Post-COVID-19 Patients Who Develop Lung Fibrotic-like Changes Have Lower Circulating Levels of IFN-β but Higher Levels of IL-1α and TGF-β

DOI: 10.3390/biomedicines9121931
	Sample: Plasma

NEPHROLOGY DIALYSIS TRANSPLANTATION (2021) IF: 5.992

Effect of fructooligosaccharide on endothelial function in CKD patients: a randomized controlled trial

DOI: 10.1093/ndt/gfaa335
	Sample: Serum

JOURNAL OF CLINICAL PERIODONTOLOGY (2020) IF: 5.241

Preparation of Buffer for WB

DOI: 10.1111/jcpe.13400
	Sample: Serum,Gingival Crevicular Fluid

Sport Sciences for Health (2022)

The effects of aerobic exercise and sertraline on pro-inflammatory indices and amelioration of neurotrophic factors in patients who have undergone CABG

DOI: 10.1007/s11332-022-00992-3
	Sample: Plasma

JOURNAL OF CLINICAL NEUROSCIENCE (2019) IF: 1.593

Sex difference in IL-6 modulation of cognition among Chinese individuals with major depressive disorder

DOI: 10.1016/j.jocn.2019.10.002
PMID: 31629608	Sample: Serum

BMC Anesthesiology (2019) IF: 1.619

A comparison of effects of scalp nerve block and local anesthetic infiltration on inflammatory response, hemodynamic response, and postoperative pain in patients undergoing craniotomy for cerebral aneurysms: a randomized controlled trial

DOI: 10.1186/s12871-019-0760-4
	Sample: Plasm

Journal of Stroke & Cerebrovascular Diseases (2020) IF: 1.787

Incremental Accuracy of Blood Biomarkers for Predicting Clinical Outcomes After Intracerebral Hemorrhage

DOI: 10.1016/j.jstrokecerebrovasdis.2020.105537
PMID: 33338706	Sample: Serum

European Journal of Obstetrics & Gynecology and Reproductive Biology (2020) IF: 1.868

A comparative study of autologous rectus fascia pubovaginal sling surgery and synthetic transobturator vaginal tape procedure in treatment of women with urodynamic stress urinary incontinence

DOI: 10.1016/j.ejogrb.2020.06.062
	Sample: Blood

AUTOIMMUNITY (2020) IF: 2.125

Auto-reactivity against gut bacterial peptides in patients with late-onset diabetes

DOI: 10.1080/08916934.2020.1818232
PMID: 32924613	Sample: Serum

BMC Neurology (2022) IF: 2.474

Changes of biochemical biomarkers in the serum of children with convulsion status epilepticus: a prospective study

DOI: 10.1186/s12883-022-02686-2
PMID: 35624413	Sample: Serum

Annals of Palliative Medicine (2021) IF: 2.595

The effects of blood purification combined with antibiotics on extravascular lung water index, inflammatory factors, and prognosis of patients with severe acute pancreatitis complicated with acute respiratory distress syndrome.

DOI: 10.21037/apm-21-2168
	Sample: Blood

PLoS One (2017) IF: 2.806

High anti-human cytomegalovirus antibody levels are associated with the progression of essential hypertension and target organ damage in Han Chinese population

DOI: 10.1371/journal.pone.0181440
	Sample: Serum

IUBMB LIFE (2015) IF: 3.143

TSG attenuates LPC‐induced endothelial cells inflammatory damage through notch signaling inhibition

DOI: 10.1002/iub.1458
	Sample: Cell Culture Supernatant

CLINICAL AND EXPERIMENTAL RHEUMATOLOGY (2018) IF: 3.201

Faecal but not serum calprotectin levels look promising in predicting active disease in Behçet's syndrome patients with gastrointestinal involvement.


PMID: 30582504	Sample: Stool

Kidney Diseases (2018) IF: 3.222

Prognostic Significance of Serum NGAL and Troponin I against Acute Kidney Injury in Egyptian ICU Patients after Open Heart Surgery: A Pilot Study

DOI: 10.1159/000490246
	Sample: Serum

NUTRITION (2021) IF: 4.008

Changes in plasma fatty acids and related biomarkers during transition to an exclusively plant- and fish-based diet in healthy adults

DOI: 10.1016/j.nut.2021.111306
PMID: 34166896	Sample: Plasma

Scientific Reports (2021) IF: 4.38

Obesity-related proteins score as a potential marker of breast cancer risk

DOI: 10.1038/s41598-021-87583-3
PMID: 33859244	Sample: Plasma

Nutrients (2020) IF: 4.546

Intermittent Fasting Enhanced the Cognitive Function in Older Adults with Mild Cognitive Impairment by Inducing Biochemical and Metabolic changes: A 3-Year Progressive Study

DOI: 10.3390/nu12092644
PMID: 32872655	Sample: Plasma

JOURNAL OF PSYCHIATRIC RESEARCH (2022) IF: 4.791

Comparison of cognitive impairments with lipid profiles and inflammatory biomarkers in unipolar and bipolar depression

DOI: 10.1016/j.jpsychires.2022.04.002
PMID: 35429740	Sample: Serum

WORLD JOURNAL OF GASTROENTEROLOGY (2021) IF: 5.742

Serum soluble suppression of tumorigenicity 2 as a novel inflammatory marker predicts the severity of acute pancreatitis

DOI: 10.3748/wjg.v27.i38.6489
PMID: 34720537	Sample: Serum

BRAIN BEHAVIOR AND IMMUNITY (2020) IF: 6.633

Differential levels of inflammatory and neuroendocrine markers in the hippocampus and anterior cingulate cortex of bipolar disorder subjects: A post-mortem study

DOI: 10.1016/j.bbi.2020.08.034
PMID: 32949690	Sample: Tissue Homogenate

Nutrients (2022) IF: 6.706

Relationship between the Dietary Inflammatory Index Score and Cytokine Levels in Chinese Pregnant Women during the Second and Third Trimesters

DOI: 10.3390/nu15010194
	Sample: Serum

ANALYTICA CHIMICA ACTA (2022) IF: 6.911

A plasmonic ELISA for multi-colorimetric sensing of C-reactive protein by using shell dependent etching of Ag coated Au nanobipyramids

DOI: 10.1016/j.aca.2022.340129
PMID: 35934405	Sample: Aunbp@Ag

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Reviews/Q&A

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Reviews
Q&A

H****ESubmitted [ Mar 07 2023 ]

Asked: What is the concentration of crp that one can expect to calculate for human SALIVA using the Elabscience ELISA (E-EL-H0043)?

adminSubmitted [ Mar 07 2023 ]

Answered: For the kit E-EL-H0043, the tested human saliva samples were about 20-125ng/mL, it is recommended to dilute at 1-10 fold.

h***** Submitted [ Mar 07 2023 ]

Asked: What is the concentration of crp that one can expect to calculate for human serum using the Elabscience ELISA (E-EL-H0043)?