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Human IFN-γ(Interferon Gamma) ELISA Kit

Citations(66)Uniprot : P01579

Cat.No.:E-EL-H0108
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T/24
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human IFN-γ in samples. No significant cross-reactivity or interference between Human IFN-γ and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human IFN-γ concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IFN-γ. You can calculate the concentration of Human IFN-γ in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 12 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IFN-γ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IFN-γ were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	51.90	98.00	447.20	55.80	100.10	476.70
Standard deviation	3.20	4.80	16.10	2.80	5.40	24.30
CV (%)	6.17	4.90	3.60	5.02	5.39	5.10

Recovery

The recovery of Human IFN-γ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	96-107	101
EDTA plasma (n=8)	89-104	96
Cell culture media (n=8)	86-97	91

Linearity

Samples were spiked with high concentrations of Human IFN-γ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P01579
Synonyms	IFNG, IFG, IFI, Type II Interferon
Research Area	Cancer, Immunology, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Journal of Tissue Engineering and Regenerative Medicine (2016) IF: 4.71

Platelet lysate supports the in vitro expansion of human periodontal ligament stem cells for cytotherapeutic use

DOI: 10.1002/term.2124
	Sample: Cell Culture Supernatant

Clinical and Translational Allergy (2022) IF: 5.871

MiR-29b regulates Th2 cell differentiation in asthma by targeting inducible B7-H3 and STAT3

DOI: 10.1002/clt2.12114
PMID: 35079347	Sample: Thp‐1 Cell

Cell Death Discovery (2021) IF: 5.241

Western Blot Guide

DOI: 10.1038/s41420-021-00401-7
PMID: 33462221	Sample: Cell Culture Supernatant

International Journal of Molecular and Cellular Medicine (2020)

MicroRNAs as Immune Regulators of Inflammation in Children with Epilepsy

DOI: 10.22088/IJMCM.BUMS.9.3.188
	Sample: Plasma

JOURNAL OF INTERNATIONAL MEDICAL RESEARCH (2020) IF: 1.287

Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells:

DOI: 10.1177/0300060520930853
	Sample: Cell Culture Supernatant

Experimental and Therapeutic Medicine (2018) IF: 1.41

Leukocyte depletion does not affect the in vitro healing ability of platelet rich plasma

DOI: 10.3892/etm.2018.5887
	Sample: Cell Culture Supernatant

ORL-Journal for Oto-Rhino-Laryngology Head and Neck Surgery (2021) IF: 1.538

Clinical Efficacy and Possible Mechanism of Endoscopic Vidian Neurectomy for House Dust Mite-Sensitive Allergic Rhinitis

DOI: 10.1159/000511711
	Sample: Serum

Dermatologic Therapy (2019) IF: 1.74

Intralesional vitamin D3 versus intralesional purified protein derivative in treatment of multiple warts: A comparative clinical and immunological study

DOI: 10.1111/dth.13034
	Sample: Serum

Journal of Thoracic Disease (2018) IF: 1.804

Programmed cell death protein-1/programmed death-ligand 1 blockade enhances the antitumor efficacy of adoptive cell therapy against non-small cell lung cancer

DOI: 10.21037/jtd.2018.10.111
PMID: 30746216	Sample: Cell Culture Supernatant

Molecular Medicine Reports (2018) IF: 1.922

The shortening telomere length of T lymphocytes maybe associated with hyper‑function in servere aplastic anemia

DOI: 10.3892/mmr.2017.8014
	Sample: Cell Culture Supernatant

BIOMARKERS (2018) IF: 1.976

Influence of IL-6, IL-10, IFN-γ and TNF-α genetic variants on susceptibility to diabetic kidney disease in type 2 diabetes mellitus patients

DOI: 10.1080/1354750X.2018.1501761
	Sample: Plasma

IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2019) IF: 2.086

In vitro impact of bisphenol A on maturation and function of monocyte-derived dendritic cells in patients with primary Sjögren’s syndrome

DOI: 10.1080/08923973.2019.1706554
	Sample: Pbmcs

LUPUS (2020) IF: 2.251

Cytokines, 25-OH vit D and disease activity in patients with juvenile-onset systemic lupus erythematosus:

DOI: 10.1177/0961203320973068
	Sample: Plasma

Biomed Research International (2020) IF: 2.276

Blood Monocyte Phenotype Fingerprint of Stable Coronary Artery Disease: A Cross-Sectional Substudy of SMARTool Clinical Trial

DOI: 10.1155/2020/8748934
	Sample: Serum

CARDIOVASCULAR PATHOLOGY (2017) IF: 2.359

C reactive protein and enzymatically modified LDL cooperatively promote dendritic cell-mediated T cell activation

DOI: 10.1016/j.carpath.2017.03.009
	Sample: Cell Culture Supernatant

BIOLOGICAL TRACE ELEMENT RESEARCH (2019) IF: 2.431

Study of Metal-Metal Interactions and Their Biomarkers Using an Intestinal Human Cell Line

DOI: 10.1007/s12011-019-01831-2
PMID: 31473895	Sample: Cell Lysate

CLINICAL CARDIOLOGY (2016) IF: 2.431

Expression of programmed cell death‐1 and its ligand B7 homolog 1 in peripheral blood lymphocytes from patients with peripartum cardiomyopathy

DOI: 10.1002/clc.22661
	Sample: Serum

INTERNATIONAL JOURNAL OF CLINICAL PRACTICE (2020) IF: 2.444

The role of the kynurenine pathway and quinolinic acid in adolescent major depressive disorder

DOI: 10.1111/ijcp.13739
PMID: 32997876	Sample: Serum

Experimental and Therapeutic Medicine (2020) IF: 2.447

Increased expression of IFN‑γ in preeclampsia impairs human trophoblast invasion via a SOCS1/JAK/STAT1 feedback loop

DOI: 10.3892/etm.2020.9544
	Sample: Tissue Homogenate

American Journal of Rhinology & Allergy (2021) IF: 2.467

Immunosuppressive Regulation of Dendritic Cells and T Cells in Allergic Rhinitis by Semaphorin 3A:

DOI: 10.1177/19458924211005592
	Sample: Cell

ACTA TROPICA (2018) IF: 2.509

Assessment of chemokine and cytokine signatures in patients with dengue infection: A hospital-based study in Kolkata, India

DOI: 10.1016/j.actatropica.2018.10.017
PMID: 30395811	Sample: Serum

DERMATOLOGIC SURGERY (2020) IF: 2.567

Efficacy of Intralesional Cryosurgery in the Treatment of Multiple Extragenital Cutaneous Warts: A Randomized Controlled Study

DOI: 10.1097/DSS.0000000000002217
	Sample: Serum

TRANSFUSION AND APHERESIS SCIENCE (2022) IF: 2.596

CFNAs of RBCs affect the release of inflammatory factors through the expression of CaMKIV in macrophages

DOI: 10.1016/j.transci.2022.103494
	Sample: Macrophages

OncoTargets and Therapy (2018) IF: 2.656

The elevated glutaminolysis of bladder cancer and T cells in a simulated tumor microenvironment contributes to the up-regulation of PD-L1 expression by interferon-γ

DOI: 10.2147/OTT.S180505
PMID: 30425515	Sample: Cell Culture Supernatant

LUPUS (2021) IF: 2.911

Association of microRNA-125a with the clinical features, disease activity and inflammatory cytokines of juvenile-onset lupus patients:

DOI: 10.1177/09612033211010328
	Sample: Plasma

CYTOKINE (2020) IF: 2.952

Involvement of the cytokine TWEAK in the pathogenesis of psoriasis vulgaris, pustular psoriasis, and erythrodermic psoriasis

DOI: 10.1016/j.cyto.2020.155391
PMID: 33302223	Sample: Serum

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2020) IF: 2.985

A novel human anti-TIGIT monoclonal antibody with excellent function in eliciting NK cell-mediated antitumor immunity

DOI: 10.1016/j.bbrc.2020.12.013
PMID: 33341068	Sample: Cell Culture Supernatant

PEDIATRIC PULMONOLOGY (2021) IF: 3.039

Maternal psychiatric status and infant wheezing: The role of maternal hormones and cord blood cytokines

DOI: 10.1002/ppul.25302
PMID: 33587823	Sample: Cord Blood(脐带血）

KIDNEY & BLOOD PRESSURE RESEARCH (2017) IF: 3.104

Association Between IFN-γ Gene Polymorphisms and IgA Nephropathy in a Chinese Han Population

DOI: 10.1159/000473889
	Sample: Serum

Oncology Letters (2022) IF: 3.111

A role for serum cytokines and cell adhesion molecules in the non‑invasive diagnosis of colorectal cancer

DOI: 10.3892/ol.2022.13443
	Sample: Serum

JOURNAL OF THE NEUROLOGICAL SCIENCES (2020) IF: 3.115

Cytokine profile during pregnancy predicts relapses during pregnancy and postpartum in multiple sclerosis

DOI: 10.1016/j.jns.2020.116811
	Sample: Serum

Biomed Research International (2022) IF: 3.246

Regulatory Effects of Fyn on Trophoblast Cell Behaviors and Function

DOI: 10.1155/2022/6006981
PMID: 36330453	Sample: Htr-8/Svneo Cells,Jeg-3 Cells

Bioengineered (2021) IF: 3.269

Exosomes loaded with programmed death ligand-1 promote tumor growth by immunosuppression in osteosarcoma

DOI: 10.1080/21655979.2021.1996509
PMID: 34699324	Sample: Jurkat Cell

NEUROLOGICAL SCIENCES (2021) IF: 3.307

Evaluation of IL-10, IFN-γ, and thiol–disulfide homeostasis in patients with drug-resistant epilepsy

DOI: 10.1007/s10072-021-05331-x
	Sample: Serum

BMC Pulmonary Medicine (2021) IF: 3.317

Systemic and local cytokine profile and risk factors for persistent allergic airway inflammation in patients sensitised to house dust mite allergens

DOI: 10.1186/s12890-021-01798-8
PMID: 34930201	Sample: Serum,Nasal Lavage

OncoTargets and Therapy (2020) IF: 3.337

MiRNA505/NET1 Axis Acts as a CD8+ T-TIL Regulator in Non-Small Cell Lung Cancer

DOI: 10.2147/OTT.S265859
	Sample: Cell Culture Supernatant

DISEASE MARKERS (2021) IF: 3.434

Genetic Polymorphisms of IFNG, IFNGR1, and Androgen Receptor and Chronic Prostatitis/Chronic Pelvic Pain Syndrome in a Chinese Han Population

DOI: 10.1155/2021/2898336
PMID: 34646402	Sample: Serum

CELL BIOLOGY INTERNATIONAL (2022) IF: 3.612

The positive feedback loop of furin and TGFβ1 enhances the immune responses of Tregs to hepatocellular carcinoma cells and hepatitis B virus in vitro

DOI: 10.1002/cbin.11806
PMID: 35349767	Sample: Hepg2 Cell

JOURNAL OF INTERFERON AND CYTOKINE RESEARCH (2022) IF: 3.657

Can the Prognosis of COVID-19 Disease Be Determined by Fecal Markers and Cytokines?

DOI: 10.1089/jir.2022.0098
PMID: 36179036	Sample: Fecal

Infectious Agents and Cancer (2023) IF: 3.698

Inhibitory effect and mechanism of Lactobacillus crispatus on cervical precancerous cells Ect1/E6E7 and screening of early warning factors

DOI: 10.1186/s13027-023-00483-1
	Sample: Ect1/E6E7 Cell

JOURNAL OF CELLULAR PHYSIOLOGY (2018) IF: 3.923

Dehydroepiandrosterone stimulates inflammation and impairs ovarian functions of polycystic ovary syndrome

DOI: 10.1002/jcp.27501
PMID: 30580448	Sample: Serum

Annals of Translational Medicine (2022) IF: 3.932

Possible causes of atherosclerosis: lncRNA COLCA1 induces oxidative stress in human coronary artery endothelial cells and impairs wound healing

DOI: 10.21037/atm-22-507
	Sample: Hcaecs

Annals of Translational Medicine (2021) IF: 3.932

Combination of oncolytic adenovirus ZD55 harboring TRAIL-IETD-MnSOD and cytokine-induced killer cells against lung cancer

DOI: 10.21037/atm-21-4479
PMID: 34790733	Sample: Serum,Beas-2B Cell

PLoS Neglected Tropical Diseases (2016) IF: 3.948

Clonorchis sinensis Co-infection Could Affect the Disease State and Treatment Response of HBV Patients

DOI: 10.1371/journal.pntd.0004806
	Sample: Serum,Cell Culture Supernatant

APOPTOSIS (2018) IF: 3.967

MiRNA-126 expression inhibits IL-23R mediated TNF-α or IFN-γ production in fibroblast-like synoviocytes in a mice model of collagen-induced rheumatoid arthritis

DOI: 10.1007/s10495-018-1474-7
	Sample: Serum

ENVIRONMENTAL TOXICOLOGY (2022) IF: 4.119

LncRNA MEG3 ameliorates NiO nanoparticles-induced pulmonary inflammatory damage via suppressing the p38 mitogen activated protein kinases pathway

DOI: 10.1002/tox.23464
PMID: 35006638	Sample: A549 Cell

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2020) IF: 4.352

S-adenosyl-L-methionine (SAMe) halts the autoimmune response in patients with primary biliary cholangitis (PBC) via antioxidant and S-glutathionylation processes in cholangiocytes

DOI: 10.1016/j.bbadis.2020.165895
PMID: 32681864	Sample: Serum

Scientific Reports (2021) IF: 4.38

Diosmectite inhibits the interaction between SARS-CoV-2 and human enterocytes by trapping viral particles, thereby preventing NF-kappaB activation and CXCL10 secretion

DOI: 10.1038/s41598-021-01217-2
PMID: 34741071	Sample: Caco-2 Cells

Journal of Oncology (2022) IF: 4.501

IL-2 Combined with IL-15 Enhanced the Expression of NKG2D Receptor on Patient Autologous NK Cells to Inhibit Wilms’ Tumor via MAPK Signaling Pathway

DOI: 10.1155/2022/4544773
	Sample: Wilms’ Tumor (Wt) Tissue

INFLAMMATION RESEARCH (2021) IF: 4.575

Molecular hydrogen alleviates asthma through inhibiting IL-33/ILC2 axis

DOI: 10.1007/s00011-021-01459-w
PMID: 33852061	Sample: Serum,Bronchoalveolar Lavage Fluid

MOLECULAR CANCER RESEARCH (2020) IF: 4.63

Glutamine Deprivation Induces PD-L1 Expression via Activation of EGFR/ERK/c-Jun Signaling in Renal Cancer

DOI: 10.1158/1541-7786.MCR-19-0517
PMID: 31672701	Sample: Cell Culture Supernatant

INTERNATIONAL JOURNAL OF CARDIOLOGY (2016) IF: 4.638

Decreased expression of programmed death 1 on peripheral blood lymphocytes disrupts immune homeostasis in peripartum cardiomyopathy

DOI: 10.1016/j.ijcard.2016.08.232
	Sample: Serum

INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932

Bufalin enhances the killing efficacy of NK cells against hepatocellular carcinoma by inhibiting MICA shedding

DOI: 10.1016/j.intimp.2021.108195
PMID: 34678691	Sample: Sk-Hep-1 Cell,Hepg2 Cell

Scientific Reports (2022) IF: 4.996

Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis

DOI: 10.1038/s41598-022-21816-x
	Sample: Serum

Frontiers in Molecular Biosciences (2021) IF: 5.246

Immunosuppression Induced by Glutamine Deprivation Occurs via Activating PD-L1 Transcription in Bladder Cancer

DOI: 10.3389/fmolb.2021.687305
PMID: 34805266	Sample: T Cells

Food & Function (2022) IF: 5.396

A randomised, double-blind, placebo-controlled trial of Bifidobacterium bifidum CCFM16 for manipulation of gut microbiota and relief from chronic constipation

DOI: 10.1039/D1FO03896F
	Sample: Serum

JOURNAL OF CELLULAR PHYSIOLOGY (2020) IF: 5.546

Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils

DOI: 10.1002/jcp.29713
PMID: 32385929	Sample: Cell Culture Supernatant

WORLD JOURNAL OF GASTROENTEROLOGY (2021) IF: 5.742

Serum soluble suppression of tumorigenicity 2 as a novel inflammatory marker predicts the severity of acute pancreatitis

DOI: 10.3748/wjg.v27.i38.6489
PMID: 34720537	Sample: Serum

Food & Function (2022) IF: 6.317

Whole grain germinated brown rice regulates intestinal immune homeostasis and gastrointestinal hormones in type 2 diabetic patients—a randomized control trial

DOI: 10.1039/D2FO00477A
	Sample: Serum

Oxidative Medicine and Cellular Longevity (2022) IF: 6.543

DNA Methyltransferase 3b Accelerates the Process of Atherosclerosis

DOI: 10.1155/2022/5249367
PMID: 35422896	Sample: Tregs

Nutrients (2022) IF: 6.706

Thymoquinone Effect on Monocyte-Derived Macrophages, Cell-Surface Molecule Expression, and Phagocytosis

DOI: 10.3390/nu14245240
	Sample: Macrophage

Nanoscale (2021) IF: 7.79

Rational design of a prodrug to inhibit self-inflammation for cancer treatment

DOI: 10.1039/D1NR00132A
PMID: 33710220	Sample: Serum,Cell Culture Supernatant(Dendritic Cells,4T1

OncoImmunology (2022) IF: 8.11

Glucose metabolism inhibitor PFK-015 combined with immune checkpoint inhibitor is an effective treatment regimen in cancer

DOI: 10.1080/2162402X.2022.2079182
PMID: 35707221	Sample: Kyse-70 Cell,Te-11 Cell

JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH (2020) IF: 11.161

MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8 + T lymphocytes in human diffuse large B cell lymphoma

DOI: 10.1186/s13046-020-01752-2
PMID: 33168024	Sample: Cell Culture Supernatant

Acta Pharmaceutica Sinica B (2021) IF: 11.614

Abrogation of HnRNP L enhances anti-PD-1 therapy efficacy via diminishing PD-L1 and promoting CD8+ T cell-mediated ferroptosis in castration-resistant prostate cancer

DOI: 10.1016/j.apsb.2021.07.016
	Sample: Pc3 Cells,Du145 Cells

Signal Transduction and Targeted Therapy (2021) IF: 18.187

Differential immune responses in pregnant patients recovered from COVID-19

DOI: 10.1038/s41392-021-00703-3
PMID: 34326311	Sample: Blood

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