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Human IGF-1(Insulin-like Growth Factor 1) ELISA Kit

Citations(18)Uniprot : P05019

Cat.No.:E-EL-H0086
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T/24
Assay time	3.5h
Detection range	1.56-100 ng/mL
Sensitivity	0.94 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human IGF-1 in samples. No significant cross-reactivity or interference between Human IGF-1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human IGF-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGF-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGF-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGF-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IGF-1. You can calculate the concentration of Human IGF-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 12 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IGF-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IGF-1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	5.30	11.80	48.60	5.60	10.90	47.70
Standard deviation	0.30	0.50	1.90	0.30	0.50	1.80
CV (%)	5.66	4.24	3.91	5.36	4.59	3.77

Recovery

The recovery of Human IGF-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-106	100
EDTA plasma (n=8)	92-108	99
Cell culture media (n=8)	86-99	92

Linearity

Samples were spiked with high concentrations of Human IGF-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P05019
Synonyms	IGF1, IGFI, IGF-I, IGF1A, IGF-IA, IGF-IB, MGF, Somatomedin C
Research Area	Cancer, Developmental biology, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Cell Death & Disease (2019) IF: 5.959

Insulin-like growth factor-1 attenuates oxidative stress-induced hepatocyte premature senescence in liver fibrogenesis via regulating nuclear p53–progerin interaction

DOI: 10.1038/s41419-019-1670-6
	Sample: Plasm

Aging-US (2020) IF: 4.831

MiR-483 induces senescence of human adipose-derived mesenchymal stem cells through IGF1 inhibition

DOI: 10.18632/aging.103818
PMID: 32805717	Sample: Cell Sample

ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES (2016) IF: 0.756

Effect of KiFAY on Performance, Insulin-like Growth Factor-1, and Thyroid Hormones in Broilers

DOI: 10.5713/ajas.16.0188
	Sample: Serum

AURIS NASUS LARYNX (2020) IF: 1.436

Association between adenotonsillar hypertrophy and leptin, ghrelin and IGF-1 levels in children

DOI: 10.1016/j.anl.2020.08.002
	Sample: Serum

Endokrynologia Polska (2021) IF: 1.582

New insights into the metabolic-bone crosstalk in active acromegaly

DOI: 10.5603/EP.a2021.0028
	Sample: Serum

GROWTH HORMONE & IGF RESEARCH (2022) IF: 2.372

In missed abortion the decrease of IGF-1 down-regulates PI3K/AKT signaling pathway reducing the secretion of progesterone and β-hCG

DOI: 10.1016/j.ghir.2022.101479
PMID: 35752133	Sample: Serum

INVESTIGATIONAL NEW DRUGS (2019) IF: 2.663

The rhenium(I)-diselenoether anticancer drug targets ROS, TGF-β1, VEGF-A, and IGF-1 in an in vitro experimental model of triple-negative breast cancers

DOI: 10.1007/s10637-019-00727-1
PMID: 30632005	Sample: Cell Culture Medium

ACTA DIABETOLOGICA (2019) IF: 2.996

polymorphisms with diabetic retinopathy in Pakistani population

DOI: 10.1007/s00592-019-01407-5
	Sample: Serum

NEUROSCIENCE LETTERS (2022) IF: 3.046

Role of IGF-1 in neuroinflammation and cognition deficits induced by sleep deprivation

DOI: 10.1016/j.neulet.2022.136575
PMID: 35276231	Sample: Plasma

DIABETES RESEARCH AND CLINICAL PRACTICE (2019) IF: 3.239

Ramadan diurnal intermittent fasting modulates SOD2, TFAM, Nrf2, and sirtuins (SIRT1, SIRT3) gene expressions in subjects with overweight and obesity

DOI: 10.1016/j.diabres.2019.107801
	Sample: Serum

DIABETES RESEARCH AND CLINICAL PRACTICE (2019) IF: 3.239

Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals

DOI: 10.1016/j.diabres.2019.05.023
PMID: 31150725	Sample: Serum

Frontiers in Nutrition (2020) IF: 3.365

A Novel 7-Days Prolonged Dietary Deprivation Regimen Improves ALT and UA After 3–6 Months Refeeding, Indicating Therapeutic Potential

DOI: 10.3389/fnut.2020.00050
	Sample: Serum

JOURNAL OF AUTISM AND DEVELOPMENTAL DISORDERS (2021) IF: 4.291

Comparison of Serum VEGF, IGF-1, and HIF-1α Levels in Children with Autism Spectrum Disorder and Healthy Controls

DOI: 10.1007/s10803-020-04820-w
PMID: 33389301	Sample: Serum

Scientific Reports (2021) IF: 4.38

Obesity-related proteins score as a potential marker of breast cancer risk

DOI: 10.1038/s41598-021-87583-3
PMID: 33859244	Sample: Plasma

Journal of Hepatocellular Carcinoma (2020) IF: 4.655

Prognostic Significance of Serum Insulin-Like Growth Factor-1 in Hepatocellular Cancer Patients: A Validation Study

DOI: 10.2147/JHC.S258930
	Sample: Serum

JOURNAL OF MOLECULAR ENDOCRINOLOGY (2023) IF: 4.869

CTCF variant begets to short stature by down-regulation of IGF-1

DOI: 10.1530/JME-22-0193
	Sample: Hek-293T Cell,Lo2 Cell,Immortalized Lymphocyte Cel

Stem Cells International (2021) IF: 5.443

Mechanical Stress-Induced IGF-1 Facilitates col-I and col-III Synthesis via the IGF-1R/AKT/mTORC1 Signaling Pathway

DOI: 10.1155/2021/5553676
PMID: 34912459	Sample: Lfc

CANCER LETTERS (2021) IF: 8.679

LncCCLM inhibits lymphatic metastasis of cervical cancer by promoting STAU1-mediated IGF-1 mRNA degradation

DOI: 10.1016/j.canlet.2021.07.005
PMID: 34273467	Sample: Cc Cells

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