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Human MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit

Citations(28)Uniprot : P13500

Cat.No.:E-EL-H6005
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T/24
Assay time	3.5h
Detection range	62.50-4000 pg/mL
Sensitivity	37.50 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human MCP-1 in samples. No significant cross-reactivity or interference between Human MCP-1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human MCP-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MCP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MCP-1. You can calculate the concentration of Human MCP-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 12 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MCP-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MCP-1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	198.91	488.33	1680.55	164.36	433.06	1730.78
Standard deviation	10.01	24.12	90.58	9.45	20.74	76.85
CV (%)	5.03	4.94	5.39	5.75	4.79	4.44

Recovery

The recovery of Human MCP-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	97-108	101
EDTA plasma (n=8)	89-99	94
Cell culture media (n=8)	87-95	92

Linearity

Samples were spiked with high concentrations of Human MCP-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P13500
Synonyms	CCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF
Research Area	Cancer, Cardiovascular, Metabolism, Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208

Protective Effects of Atractylodis lancea Rhizoma on Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo

DOI: 10.3390/ijms232416134
	Sample: Thp-1 Cells

Therapeutic Advances in Ophthalmology (2020)

Serum malondialdehyde, monocyte chemoattractant protein-1, and vitamin C levels in wet type age-related macular degeneration patients:

DOI: 10.1177/2515841420951682
	Sample: Serum

International Journal of Clinical and Experimental Pathology (2018) IF: 1.396

MicroRNA-124 alleviates hyperoxia-induced inflammatory response in pulmonary epithelial cell by inhibiting TLR4/NF-κB/CCL2


PMID: 31938089	Sample: Beas2B Cells

Experimental and Therapeutic Medicine (2020) IF: 1.785

Curcumin affects ox‑LDL‑induced IL‑6, TNF‑α, MCP‑1 secretion and cholesterol efflux in THP‑1 cells by suppressing the TLR4/NF‑κB/miR33a signaling pathway

DOI: 10.3892/etm.2020.8915
	Sample: Cell Culture Supernatant

Trials (2021) IF: 2.279

Effect of Lycium barbarum polysaccharide supplementation in non-alcoholic fatty liver disease patients: study protocol for a randomized controlled trial

DOI: 10.1186/s13063-021-05529-6
PMID: 34521466	Sample: Blood

GENE (2019) IF: 2.638

Downregulation of hsa_circ_0068087 ameliorates TLR4/NF-κB/NLRP3 inflammasome-mediated inflammation and endothelial cell dysfunction in high glucose conditioned by sponging miR-197

DOI: 10.1016/j.gene.2019.05.012
	Sample: Human Umbilical Vein Endothelial Cells (Huvecs)

PLoS One (2018) IF: 2.766

Salivary immunity and lower respiratory tract infections in non-elite marathon runners

DOI: 10.1371/journal.pone.0206059
PMID: 30462646	Sample: Saliva

PLoS One (2017) IF: 2.806

Depot-specific inflammation with decreased expression of ATM2 in white adipose tissues induced by high-margarine/lard intake

DOI: 10.1371/journal.pone.0188007
	Sample: Cell Culture Supernatant

NEUROTOXICITY RESEARCH (2020) IF: 2.992

The Neuroimmune and Neurotoxic Fingerprint of Major Neurocognitive Psychosis or Deficit Schizophrenia: a Supervised Machine Learning Study

DOI: 10.1007/s12640-019-00112-z
	Sample: Serum

INFLAMMATION RESEARCH (2019) IF: 3.061

Common variants of genes encoding TLR4 and TLR4 pathway members TIRAP and IRAK1 are effective on MCP1, IL6, IL1β, and TNFα levels in type 2 diabetes and insulin resistance

DOI: 10.1007/s00011-019-01263-7
	Sample: Serum

Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2021) IF: 3.168

Inflammatory Biomarkers Levels in T2DM Emirati Patients with Diabetic Neuropathy

DOI: 10.2147/DMSO.S319863
PMID: 34345175	Sample: Serum

American Journal of Translational Research (2019) IF: 3.266

Profiling and functional characterization of circulation LncRNAs that are associated with coronary atherosclerotic plaque stability


PMID: 31312390	Sample: Thp-1 Cells

SCHIZOPHRENIA RESEARCH (2020) IF: 3.759

Chronic fatigue and fibromyalgia symptoms are key components of deficit schizophrenia and are strongly associated with activated immune-inflammatory pathways

DOI: 10.1016/j.schres.2020.05.003
PMID: 32467068	Sample: Serum

BIOSCIENCE REPORTS (2019) IF: 3.84

Up-regulated MCPIP1 in abdominal aortic aneurysm is associated with vascular smooth muscle cell apoptosis and MMPs production

DOI: 10.1042/BSR20191252
PMID: 31651935	Sample: Serum,Plasma,Cell Sample

Journal of the International Society of Sports Nutrition (2019) IF: 3.841

Effects of a polysaccharide-based multi-ingredient supplement on salivary immunity in non-elite marathon runners

DOI: 10.1186/s12970-019-0281-z
	Sample: Saliva

Scientific Reports (2020) IF: 3.998

Intermittent hypoxia modulates redox homeostasis, lipid metabolism associated inflammatory processes and redox post-translational modifications: Benefits at high altitude

DOI: 10.1038/s41598-020-64848-x
PMID: 32404929	Sample: Plasma

Drug Design Development and Therapy (2021) IF: 4.162

Prunella vulgaris L. Attenuates Experimental Autoimmune Thyroiditis by Inhibiting HMGB1/TLR9 Signaling

DOI: 10.2147/DDDT.S325814
PMID: 34764638	Sample: Nthy-Ori 3–1 Cells

JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36

Gynura procumbens (Lour.) Merr. extract attenuates monocyte adherence to endothelial cells through suppression of the NF-κB signaling pathway

DOI: 10.1016/j.jep.2022.115391
PMID: 35589022	Sample: Huvecs

INFLAMMATION RESEARCH (2021) IF: 4.575

Molecular hydrogen alleviates asthma through inhibiting IL-33/ILC2 axis

DOI: 10.1007/s00011-021-01459-w
PMID: 33852061	Sample: Serum,Bronchoalveolar Lavage Fluid

ADDICTION BIOLOGY (2017) IF: 4.603

Young alcohol binge drinkers have elevated blood endotoxin, peripheral inflammation and low cortisol levels: neuropsychological correlations in women

DOI: 10.1111/adb.12543
	Sample: Plasma

Cancers (2022) IF: 6.575

The GBM Tumor Microenvironment as a Modulator of Therapy Response: ADAM8 Causes Tumor Infiltration of Tams through HB-EGF/EGFR-Mediated CCL2 Expression and Overcomes TMZ Chemosensitization in Glioblastoma

DOI: 10.3390/cancers14194910
PMID: 36230833	Sample: U87Mg Cell,G1 Cell

Nutrients (2023) IF: 6.706

Safety and Effects of Lactobacillus paracasei TISTR 2593 Supplementation on Improving Cholesterol Metabolism and Atherosclerosis-Related Parameters in Subjects with Hypercholesterolemia: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial

DOI: 10.3390/nu15030661
	Sample: Serum

Oxidative Medicine and Cellular Longevity (2022) IF: 7.31

Antidyslipidemic, Antioxidant, and Anti-inflammatory Effects of Jelly Drink Containing Polyphenol-Rich Roselle Calyces Extract and Passion Fruit Juice with Pulp in Adults with Dyslipidemia: A Randomized, Double-Blind, Placebo-Controlled Trial.

DOI: 10.1155/2022/4631983
	Sample: Plasma

Cell Death & Disease (2020) IF: 8.469

Intracellular virus sensor MDA5 exacerbates vitiligo by inducing the secretion of chemokines in keratinocytes under virus invasion

DOI: 10.1038/s41419-020-2665-z
PMID: 32532953	Sample: Cell Culture Supernatant

CLINICAL CANCER RESEARCH (2019) IF: 8.911

Tumor-Contacted Neutrophils Promote Metastasis by a CD90-TIMP-1 Juxtacrine–Paracrine Loop

DOI: 10.1158/1078-0432.CCR-18-2544
	Sample: Tumor-Infiltrating Neutrophils (Tins),Neutrophils

THROMBOSIS RESEARCH (2022) IF: 10.407

High mobility group box 1 derived mainly from platelet microparticles exacerbates microvascular obstruction in no reflow

DOI: 10.1016/j.thromres.2022.12.003
	Sample: Human Cardiac Microvascular Endothelial Cells (Hcm

Theranostics (2021) IF: 11.556

A SCID mouse-human lung xenograft model of SARS-CoV-2 infection

DOI: 10.7150/thno.58321
PMID: 33995679	Sample: Tissue Homogenate

Molecular Cancer (2021) IF: 27.401

KDM6A-ARHGDIB axis blocks metastasis of bladder cancer by inhibiting Rac1

DOI: 10.1186/s12943-021-01369-9
PMID: 34006303	Sample: Cell Culture Supernatant

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