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Human S100B(S100 Calcium Binding Protein B) ELISA Kit

Citations(12)Uniprot : P04271

Cat.No.:E-EL-H1297
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T/24
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human S100B in samples. No significant cross-reactivity or interference between Human S100B and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human S100B concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human S100B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human S100B. You can calculate the concentration of Human S100B in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 12 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human S100B were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human S100B were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	104.40	168.30	729.10	107.50	175.90	681.00
Standard deviation	7.10	7.70	25.50	5.80	8.30	32.00
CV (%)	6.80	4.58	3.50	5.40	4.72	4.70

Recovery

The recovery of Human S100B spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-98	93
EDTA plasma (n=8)	93-108	100
Cell culture media (n=8)	95-112	102

Linearity

Samples were spiked with high concentrations of Human S100B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P04271
Synonyms	S100-B, NEF, S100Beta
Research Area	Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Advanced Pharmaceutical Bulletin (2021)

Targeting Mitochondrial and Brain Injury Markers in Acquired Brain Injuries: A Randomized, Double-Blind, Placebo-Controlled Study with Melatonin

DOI: 10.34172/apb.2022.013
	Sample: Serum

MEDICINE (2021) IF: 1.889

Serum NSE and S100B protein levels for evaluating the impaired consciousness in patients with acute carbon monoxide poisoning

DOI: 10.1097/MD.0000000000026458
PMID: 34160445	Sample: Serum

BRAIN INJURY (2021) IF: 2.311

Changes in amplitude-integrated electroencephalography, neuron-specific enolase, and S100B in neonates with brain injury induced by neonatal hyperbilirubinemia and their significance

DOI: 10.1080/02699052.2021.1931449
PMID: 34097553	Sample: Serum

NEUROLOGICAL SCIENCES (2020) IF: 2.415

The elevation of S100B and downregulation of circulating miR-602 in the sera of ischemic stroke (IS) patients: the emergence of novel diagnostic and prognostic markers

DOI: 10.1007/s10072-020-04323-7
	Sample: Serum

JOURNAL OF INTELLECTUAL DISABILITY RESEARCH (2021) IF: 2.424

Association of BDNF levels with IQ: comparison of S100B and BDNF levels in typically developing children and subjects with neurologically normal nonsyndromic intellectual disability

DOI: 10.1111/jir.12896
PMID: 34750906	Sample: Serum

BMC Neurology (2022) IF: 2.474

Changes of biochemical biomarkers in the serum of children with convulsion status epilepticus: a prospective study

DOI: 10.1186/s12883-022-02686-2
PMID: 35624413	Sample: Serum

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES (2019) IF: 2.591

Enteroviral meningitis reduces CSF concentration of Aβ42, but does not affect markers of parenchymal damage

DOI: 10.1007/s10096-019-03569-0
	Sample: Cerebrospinal Fluid(Csf)

JOURNAL OF THE NEUROLOGICAL SCIENCES (2022) IF: 3.181

Plasma biomarkers of brain injury in COVID-19 patients with neurological symptoms

DOI: 10.1016/j.jns.2022.120324
PMID: 35752131	Sample: Serum

MEDIATORS OF INFLAMMATION (2017) IF: 3.232

Short-Term Postoperative Cognitive Dysfunction and Inflammatory Response in Patients Undergoing Cytoreductive Surgery and Hyperthermic Intraperitoneal Chemotherapy: A Pilot Study

DOI: 10.1155/2017/3605350
	Sample: Serum

JOURNAL OF INTERFERON AND CYTOKINE RESEARCH (2022) IF: 3.657

Can the Prognosis of COVID-19 Disease Be Determined by Fecal Markers and Cytokines?

DOI: 10.1089/jir.2022.0098
PMID: 36179036	Sample: Fecal

Frontiers in Neurology (2022) IF: 4.086

Neurofilament light chain and S100B serum levels are associated with disease severity and outcome in patients with aneurysmal subarachnoid hemorrhage

DOI: 10.3389/fneur.2022.956043
PMID: 35989914	Sample: Serum

Nutrients (2021) IF: 5.719

Rapid and Effective Vitamin D Supplementation May Present Better Clinical Outcomes in COVID-19 (SARS-CoV-2) Patients by Altering Serum INOS1, IL1B, IFNg, Cathelicidin-LL37, and ICAM1

DOI: 10.3390/nu13114047
PMID: 34836309	Sample: Serum

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