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Human TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

  • Cat.No.:E-EL-H0109

  • Reactivity: Human

To Purchase E-EL-H0109

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 7.81-500 pg/mL
Sensitivity 4.69 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human TNF-α in samples. No significant cross-reactivity or interference between Human TNF-α and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human TNF-α concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TNF-α. You can calculate the concentration of Human TNF-α in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TNF-α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TNF-α were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 24.10 79.50 183.00 22.70 86.50 177.40
Standard deviation 1.50 3.80 9.70 1.20 4.00 7.60
CV (%) 6.22 4.78 5.30 5.29 4.62 4.28


The recovery of Human TNF-α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-100 92
EDTA plasma (n=8) 94-106 99
Cell culture media (n=8) 93-107 99


Samples were spiked with high concentrations of Human TNF-α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P01375
SynonymsDIF, TNF-alpha, TNFA, TNFSF2
Research AreaCancer, Cardiovascular, Metabolism, Immunology, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

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  1. Cell (2020) IF: 38.637
    Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output

    DOI: 10.1016/j.cell.2020.08.009

    Sample: Cell culture medium
  2. Hepatology (2020) IF: 14.679
    Increased Liver Localization of Lipopolysaccharides in Human and Experimental NAFLD

    DOI: 10.1002/hep.31056

    PMID: 31808577

    Sample: Serum
  3. Acs Nano (2017) IF: 13.942
    Overcoming The Heat Endurance Of Tumor Cells By Interfering The Anaerobic Glycolysis Metabolism For Improved Photothermal Therapy.

    DOI: 10.1021/acsnano.6b06658

    PMID: 28107631

    Sample: Cell culture supernatant
  4. Theranostics (2021) IF: 11.556
    A SCID mouse-human lung xenograft model of SARS-CoV-2 infection

    DOI: 10.7150/thno.58321

    PMID: 33995679

    Sample: Tissue homogenate
  5. Cell Death & Disease (2021) IF: 8.4689
    Circular RNA circACSL1 aggravated myocardial inflammation and myocardial injury by sponging miR-8055 and regulating MAPK14 expression

    DOI: 10.1038/s41419-021-03777-7

    PMID: 33986259

    Sample: Cell culture supernatant
  6. Carbohydrate Polymers (2020) IF: 7.182
    Dual functionalized chitosan based composite hydrogel for haemostatic efficacy and adhesive property

    DOI: 10.1016/j.carbpol.2020.116757

    PMID: 32829870

    Sample: Cell culture supernatant
  7. OncoImmunology (2017) IF: 7.719
    Exploiting A New Strategy To Induce Immunogenic Cell Death To Improve Dendritic Cell-Based Vaccines For Lymphoma Immunotherapy.

    DOI: 10.1080/2162402X.2017.1356964

    PMID: 29147614

    Sample: Cell culture supernatant
  8. Biomedicine & Pharmacotherapy (2021) IF: 6.529
    Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity

    DOI: 10.1016/j.biopha.2021.111531

    PMID: 34311530

    Sample: Serum
  9. BMC Research Notes (2020) IF: 6.782
    Green coffee extract modifies body weight, serum lipids and TNF-α in high-fat diet-induced obese rats

    DOI: 10.1186/s13104-020-05052-y

    PMID: 32276657

    Sample: Serum
  10. EBioMedicine (2018) IF: 6.183
    The enhancer RNA lnc-SLC4A1-1 epigenetically regulates unexplained recurrent pregnancy loss (URPL) by activating CXCL8 and NF-kB pathway

    DOI: 10.1016/j.ebiom.2018.11.015

    PMID: 30448228

    Sample: Cell culture supernatant
  11. Journal of Cellular Physiology (2020) IF: 5.546
    Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils

    DOI: 10.1002/jcp.29713

    PMID: 32385929

    Sample: Cell culture supernatant
  12. Liver International (2020) IF: 5.175
    Fontan protein-losing enteropathy is associated with advanced liver disease and a proinflammatory intestinal and systemic state

    DOI: 10.1111/liv.14375

    PMID: 31912956

    Sample: Plasma
  13. Clinical Science (2020) IF: 5.223
    Effects of circulating extracellular microvesicles from spinal cord-injured adults on endothelial cell function

    DOI: 10.1042/CS20200047

    PMID: 32219341

    Sample: Cell culture supernatant
  14. Osteoarthritis and cartilage (2018) IF: 5.454
    Associations between knee structural measures, circulating inflammatory factors and MMP13 in patients with knee osteoarthritis.

    DOI: 10.1016/j.joca.2018.05.003

    PMID: 29753949

    Sample: Serum
  15. The FASEB Journal (2020) IF: 4.966
    Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output

    DOI: 10.1016/j.cell.2020.08.009

    PMID: 32931733

    Sample: Cell culture supernatant
  16. Journal of Reproductive Immunology (2020) IF: 4.018
    Inhibition of TLR2/TLR4 alleviates the Neisseria gonorrhoeae infection damage in human endometrial epithelial cells via Nrf2 and NF-Kβsignaling

    DOI: 10.1016/j.jri.2020.103192

    PMID: 32950783

    Sample: Cell culture supernatant
  17. Biomedicine & Pharmacotherapy (2020) IF: 4.545
    LncRNA SNHG5 regulates cell apoptosis and inflammation by miR-132/PTEN axis in COPD

    DOI: 10.1016/j.biopha.2020.110016

    PMID: 32145584

    Sample: Cell culture supernatant
  18. Biomedicine & Pharmacotherapy (2020) IF: 4.545
    Long non-coding RNA NNT-AS1 regulates proliferation, apoptosis, inflammation and airway remodeling of chronic obstructive pulmonary disease via targeting miR-582-5p/FBXO11 axis

    DOI: 10.1016/j.biopha.2020.110326

    Sample: Cell culture supernatant
  19. Diabetes research and clinical practice (2019) IF: 4.234
    Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals

    DOI: 10.1016/j.diabres.2019.05.023

    PMID: 31150725

    Sample: Serum
  20. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2019) IF: 4.328
    Inflammation-dependent ISG15 upregulation mediates MIA-induced dendrite damages and depression by disrupting NEDD4/Rap2A signaling

    DOI: 10.1016/j.bbadis.2019.02.020

    PMID: 30826466

    Sample: Serum
  21. International Journal of Nanomedicine (2017) IF: 4.3
    Zinc Oxide Nanoparticle-Induced Atherosclerotic Alterations In Vitro And In Vivo.

    DOI: 10.2147/IJN.S134897

    PMID: 28652743

    Sample: Cell culture supernatant,Serum
  22. Oxidative Medicine and Cellular Longevity (2017) IF: 4.593
    Ros-Dependent Activation Of Autophagy Through The PI3K/Akt/mTOR Pathway Is Induced By Hydroxysafflor Yellow A-Sonodynamic Therapy In THP-1 Macrophages.

    DOI: 10.1155/2017/8519169

    PMID: 28191279

    Sample: Cell culture supernatant
  23. Scientific Reports (2015) IF: 4.259
    The Role Of miR-19b In The Inhibition Of Endothelial Cell Apoptosis And Its Relationship With Coronary Artery Disease.

    DOI: 10.1038/srep15132

    PMID: 26459935

    Sample: Serum
  24. Bioconjugate Chemistry (2016) IF: 4.818
    Multifunctional Dextran Sulfate-Coated Reconstituted High Density Lipoproteins Target Macrophages And Promote Beneficial Antiatherosclerotic Mechanisms.

    DOI: 10.1021/acs.bioconjchem.6b00600

    PMID: 28004910

    Sample: Cell culture supernatant
  25. Frontier in Cellular and Infection Microbiology (2016) IF: 4.3
    Positive Feedback Cycle Of TNF-α Promotes Staphylococcal Enterotoxin B-Induced THP-1 Cell Apoptosis.

    DOI: 10.3389/fcimb.2016.00109

    PMID: 27709104

    Sample: Cell culture supernatant
  26. Scientific reports (2020) IF: 3.998
    Early modulation of macrophage ROS-PPARγ-NF-κB signalling by sonodynamic therapy attenuates neointimal hyperplasia in rabbits

    DOI: 10.1038/s41598-020-68543-9

    PMID: 32669704

    Sample: Cell culture supernatant
  27. Fish & Shellfish Immunology (2020) IF: 3.298
    Molecular characterization, inducible expression and functional analysis of an IKKβ from the tropical sea cucumber Holothuria leucospilota

    DOI: 10.1016/j.fsi.2020.06.024

    PMID: 32585358

    Sample: Cell culture supernatant
  28. European Journal of Pharmacology (2020) IF: 3.263
    iTRAQ-based proteomics and in vitro experiments reveals essential roles of ACE and AP-N in the renin-angiotensin system-mediated congenital ureteropelvic junction obstruction

    DOI: 10.1016/j.yexcr.2020.112086

    PMID: 32416091

    Sample: Serum,Cell sample
  29. Journal of Ethnopharmacology (2020) IF: 3.69
    Anti-angiogenic effect of Shikonin in rheumatoid arthritis by downregulating PI3K/AKT and MAPKs signaling pathways

    DOI: 10.1016/j.jep.2020.113039

    PMID: 32497675

    Sample: Serum,Cell culture supernatant
  30. European review for medical and pharmacological sciences (2020) IF: 3.024
    miR-942-5p alleviates septic acute kidney injury by targeting FOXO3

    DOI: 10.26355/eurrev_202006_21521

    PMID: 32572890

    Sample: Cell culture supernatant
  31. Journal of Ethnopharmacology (2020) IF: 3.69
    Notopterygium incisum extract (NRE) rescues cognitive deficits in APP/PS1 Alzhneimer's disease mice by attenuating amyloid-beta, tau, and neuroinflammation pathology

    DOI: 10.1016/j.jep.2019.112433

    PMID: 31783135

    Sample: Cell culture supernatant
  32. Inflammopharmacology (2020) IF: 3.238
    Tocilizumab attenuates acute lung and kidney injuries and improves survival in a rat model of sepsis via down-regulation of NF-κB/JNK: a possible role of P-glycoprotein

    DOI: 10.1007/s10787-019-00628-y

    PMID: 31440860

    Sample: Serum
  33. Investigative ophthalmology & visual science (2020) IF: 3.47
    Perillaldehyde Ameliorates Aspergillus fumigatus Keratitis by Activating the Nrf2/HO-1 Signaling Pathway and Inhibiting Dectin-1-Mediated Inflammation

    DOI: 10.1167/iovs.61.6.51

    PMID: 32579678

    Sample: Cell lysate,Cell culture medium
  34. Schizophrenia Research (2020) IF: 3.759
    Chronic fatigue and fibromyalgia symptoms are key components of deficit schizophrenia and are strongly associated with activated immune-inflammatory pathways

    DOI: 10.1016/j.schres.2020.05.003

    PMID: 32467068

    Sample: Serum
  35. European review for medical and pharmacological sciences (2019) IF: 3.024
    Plasma concentration of ZCCHC14 contributes to prognostic efficacy in intracerebral hemorrhage patients

    DOI: 10.26355/eurrev_201910_19167

    PMID: 31646585

    Sample: Plasma
  36. Apoptosis (2018) IF: 3.967
    miR-A-126 expression inhibits IL-23R mediated TNF-α or IFN-γ production in fibroblast-like synoviocytes in a mice model of collagen-induced rheumatoid arthritis

    DOI: 10.1007/s10495-018-1474-7

    PMID: 30167920

    Sample: Serum
  37. International immunopharmacology (2019) IF: 3.118
    S1P promotes inflammation-induced tube formation by HLECs via the S1PR1/NF-κB pathway

    DOI: 10.1016/j.intimp.2018.11.032

    PMID: 30476824

    Sample: Cell culture supernatant
  38. Life sciences (2019) IF: 3.234
    LncRNA PMS2L2 protects ATDC5 chondrocytes against lipopolysaccharide-induced inflammatory injury by sponging miR-203

    DOI: 10.1016/j.lfs.2018.12.020

    PMID: 30550887

    Sample: Cell culture supernatant
  39. Molecular immunology (2019) IF: 3.188
    IL-16 regulates macrophage polarization as a target gene of miR-145-3p

    DOI: 10.1016/j.molimm.2018.12.027

    PMID: 30634164

    Sample: Cell lysate
  40. Biomedicine & Pharmacotherapy (2019) IF: 3.457
    Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro

    DOI: 10.1016/j.biopha.2019.108706

    PMID: 30844656

    Sample: Wound drainage fluid
  41. Biomedicine & Pharmacotherapy (2018) IF: 3.457
    Biochemical investigation of gender-specific association between insulin resistance and inflammatory biomarkers in types 2 diabetic patients.

    DOI: 10.1016/j.biopha.2018.06.044

    PMID: 29966972

    Sample: Serum
  42. Mediators Of Inflammation (2017) IF: 3.232
    Short-Term Postoperative Cognitive Dysfunction And Inflammatory Response In Patients Undergoing Cytoreductive Surgery And Hyperthermic Intraperitoneal Chemotherapy: A Pilot Study.

    DOI: 10.1155/2017/3605350

    PMID: 29249869

    Sample: Serum
  43. International Journal of Molecular Sciences (2017) IF: 3.226
    The Pharmaceutical Device Prisma Skin Promotes In Vitro Angiogenesis Through Endothelial To Mesenchymal Transition During Skin Wound Healing.

    DOI: 10.3390/ijms18081614

    PMID: 28757565

    Sample: Cell culture supernatant
  44. Apoptosis (2015) IF: 3.833
    In Vitro Antiproliferative, Pro-Apoptotic, Antimetastatic And Anti-Inflammatory Potential Of 2, 4-Diacteylphloroglucinol (DAPG) By Pseudomonas Aeruginosa Strain FP10.

    DOI: 10.1007/s10495-015-1162-9

    PMID: 26283170

    Sample: Cell culture supernatant
  45. World Journal of Gastroenterology (2016) IF: 3.365
    Inhibitory Effect Of miR-125b On Hepatitis C Virus Core Protein-Induced TLR2/MyD88 Signaling In THP-1 Macrophages.

    DOI: 10.3748/wjg.v22.i17.4354

    PMID: 27158204

    Sample: Cell culture supernatant
  46. Sleep and Breathing (2020) IF: 2.162
    Intermittent hypoxia induces tumor immune escape in murine S180 solid tumors via the upregulation of TGF-β 1 in mice

    DOI: 10.1007/s11325-020-02166-2

    PMID: 32840731

    Sample: Tissue homogenate
  47. Pediatric Pulmonology (2020) IF: 2.534
    Obstructive sleep apnea in children with hypothalamic obesity: Evaluation of possible related factors

    DOI: 10.1002/ppul.25097

    PMID: 32986303

    Sample: Plasma
  48. Human & Experimental Toxicology (2020) IF: 2.067
    Investigation of DNA damage, oxidative stress, and inflammation in synthetic cannabinoid users

    DOI: 10.1177/0960327120930057

    PMID: 32508150

    Sample: Plasma
  49. Autoimmunity (2020) IF: 2.125
    Auto-reactivity against gut bacterial peptides in patients with late-onset diabetes

    DOI: 10.1080/08916934.2020.1818232

    PMID: 32924613

    Sample: Serum
  50. Archives of Physiology and Biochemistry (2020) IF: 2.575
    XIST silencing alleviated inflammation and mesangial cells proliferation in diabetic nephropathy by sponging miR-485

    DOI: 10.1080/13813455.2020.1789880

    PMID: 32669002

    Sample: Serum
  51. Journal of Zhejiang University. Science. B (2020) IF: 2.082
    Clinical characteristics of 34 COVID-19 patients admitted to intensive care unit in Hangzhou, China

    DOI: 10.1631/jzus.B2000174

    PMID: 32425003

    Sample: Serum,Plasma
  52. Biological Trace Element Research (2019) IF: 2.639
    Study of Metal-Metal Interactions and Their Biomarkers Using an Intestinal Human Cell Line

    DOI: 10.1007/s12011-019-01831-2

    PMID: 31473895

    Sample: Cell lysate
  53. Biochemical and Biophysical Research Communications (2020) IF: 2.985
    Long non-coding RNA MEG3 regulates CSE-induced apoptosis and inflammation via regulating miR-218 in 16HBE cells

    DOI: 10.1016/j.bbrc.2019.10.135

    PMID: 31668807

    Sample: Cell culture supernatant
  54. Scandinavian Journal of Immunology (2020) IF: 2.717
    Early HIV infection is associated with reduced proportions of gamma delta T subsets as well as high creatinine and urea levels

    DOI: 10.1111/sji.12868

    PMID: 32052490

    Sample: Serum
  55. Gastroenterology Report (2020) IF: 2.96
    Effect of Helicobacter pylori-eradication therapy on hepatic steatosis in patients with non-alcoholic fatty liver disease: a randomized-controlled pilot study

    DOI: 10.1093/gastro/goz058

    PMID: 32499918

    Sample: Serum
  56. Poultry Science (2020) IF: 2.659
    The effect of different dietary ratios of arginine, methionine, and lysine on the performance, carcass traits, and immune status of turkeys

    DOI: 10.1016/j.psj.2019.10.008

    PMID: 32036960

    Sample: Plasma
  57. Neurotoxicity research (2020) IF: 2.992
    Major Depression in Children with Transfusion-Dependent Thalassemia Is Strongly Associated with the Combined Effects of Blood Transfusion Rate, Iron Overload, and Increased pro-inflammatory Cytokines

    DOI: 10.1007/s12640-020-00193-1

    PMID: 32335809

    Sample: Serum
  58. Microbial pathogenesis (2019) IF: 2.914
    Isolation, characterization and analysis of pro-inflammatory potential of Klebsiella pneumoniae outer membrane vesicles

    DOI: 10.1016/j.micpath.2019.103719

    PMID: 31493501

    Sample: Cell culture supernatant
  59. Apmis (2019) IF: 2.026
    Autoantibodies to a Specific Peptide Epitope of Human Hsp60 (ATVLA) With Homology to Helicobacter Pylori HspB in H. Pylori-Infected Patients

    DOI: 10.1111/apm.12925

    PMID: 30746790

    Sample: Cell culture supernatant
  60. Evidence-Based Complementary and Alternative Medicine (2019) IF: 2.064
    In Vitro Immunomodulatory Effect of Food Supplement from Aloe vera

    DOI: 10.1155/2019/5961742

    PMID: 30941196

    Sample: Cell culture supernatant
  61. Biomedicine & Pharmacotherapy (2018) IF: 2.759
    Oleanolic acid protects against pathogenesis of atherosclerosis, possibly via FXR-mediated angiotensin (Ang)-(1-7) upregulation.

    DOI: 10.1016/j.biopha.2017.11.151

    PMID: 29793333

    Sample: Cell culture supernatant,Serum
  62. Plos One (2017) IF: 2.806
    High Anti-Human Cytomegalovirus Antibody Levels Are Associated With The Progression Of Essential Hypertension And Target Organ Damage In Han Chinese Population.

    DOI: 10.1371/journal.pone.0181440

    PMID: 28837559

    Sample: Serum
  63. Cardiovascular Pathology (2017) IF: 2.359
    C Reactive Protein And Enzymatically Modified LDL Cooperatively Promote Dendritic Cell-Mediated T Cell Activation.

    DOI: 10.1016/j.carpath.2017.03.009

    PMID: 28433861

    Sample: Cell culture supernatant
  64. Molecules (2015) IF: 2.861
    Mitochondrion-Targeted Peptide SS-31 Inhibited Oxidized Low-Density Lipoproteins-Induced Foam Cell Formation Through Both ROS Scavenging And Inhibition Of Cholesterol Influx In Raw264.7 Cells.

    DOI: 10.3390/molecules201219764

    PMID: 26633327

    Sample: Cell lysate
  65. Toxicology in Vitro (2016) IF: 2.866
    Sublethal Effects Of Zinc Oxide Nanoparticles On Male Reproductive Cells.

    DOI: 10.1016/j.tiv.2016.05.017

    PMID: 27247145

    Sample: Cell culture supernatant
  66. International Journal of Infectious Diseases (2015) IF: 2.532
    Decreased Monocyte Subsets And TLR4-Mediated Functions In Patients With Acute Severe Fever With Thrombocytopenia Syndrome (SFTS).

    DOI: 10.1016/j.ijid.2015.12.009

    PMID: 26701820

    Sample: Cell culture supernatant
  67. Plos One (2013) IF: 2.806
    Oxidative Burst Of Circulating Neutrophils Following Traumatic Brain Injury In Human.

    DOI: 10.1371/journal.pone.0068963

    PMID: 23894384

    Sample: Plasma
  68. Evidence-Based Complementary and Alternative Medicine (2020) IF: 1.813
    Rhubarb-Aconite Decoction (RAD) Drug-Containing Serum Alleviated Endotoxin-Induced Oxidative Stress Injury and Inflammatory Response in Caco-2 Cells In Vitro

    DOI: 10.1155/2020/5834502

    PMID: 32714409

    Sample: Cell culture supernatant
  69. Archives of Oral Biology (2020) IF: 1.931
    Nec-1 attenuates inflammation and cytotoxicity induced by high glucose on THP-1 derived macrophages through RIP1

    DOI: 10.1016/j.archoralbio.2020.104858

    PMID: 32805637

    Sample: Cell culture supernatant
  70. Experimental and therapeutic medicine (2020) IF: 1.785
    Mycobacterium tuberculosis infection increases the number of osteoclasts and inhibits osteoclast apoptosis by regulating TNF-α-mediated osteoclast autophagy

    DOI: 10.3892/etm.2020.8903

    PMID: 32782497

    Sample: Cell culture supernatant
  71. Genetic Testing and Molecular Biomarkers (2020) IF: 1.346
    Correlation Between Single Nucleotide Polymorphisms at the 3'-UTR of the NFKB1 Gene and Acute Kidney Injury in Sepsis

    DOI: 10.1089/gtmb.2019.0222

    PMID: 32315555

    Sample: Cell sample
  72. Medical Science Monitor (2020) IF: 1.918
    Avicularin Reduces the Expression of Mediators of Inflammation and Oxidative Stress in Bradykinin-Treated MG-63 Human Osteoblastic Osteosarcoma Cells

    DOI: 10.12659/MSM.921957

    PMID: 32463805

    Sample: Cell culture supernatant
  73. Experimental and Therapeutic Medicine (2020) IF: 1.785
    Systematically transplanted human gingiva-derived mesenchymal stem cells regulate lipid metabolism and inflammation in hyperlipidemic mice with periodontitis

    DOI: 10.3892/etm.2019.8256

    PMID: 31885706

    Sample: Serum
  74. ANZ Journal of Surgery (2020) IF: 1.355
    Effect of laparoscopic sleeve gastrectomy on renal function in obese patients

    DOI: 10.1111/ans.15767

    PMID: 32115876

    Sample: Serum,Urine
  75. International Journal of Environmental Analytical Chemistry (2020) IF: 1.431
    A label-free and sensitive impedimetric immunosensor for TNF-α biomarker detection based on epoxysilane-modified disposable ITO-PET electrode

    DOI: 10.1080/03067319.2019.1679807

    Sample: Immunosensor Construction
  76. medicine (2019) IF: 1.552
    A case-control study on the relationship between miR-As single nucleotide polymorphisms and sepsis risk

    DOI: 10.1097/MD.0000000000016744

    PMID: 31415369

    Sample: Serum
  77. Genetic testing and molecular biomarkers (2018) IF: 1.181
    Association of High Mobility Group Box Protein B1 Gene Polymorphisms with Pneumonia Susceptibility and Severity

    DOI: 10.1089/gtmb.2018.0174

    PMID: 30562142

    Sample: Plasma
  78. Journal of Burn Care & Research (2019) IF: 1.923
    Mitochondrial DNA-Induced Inflammatory Responses and Lung Injury in Thermal Injury Murine Model: Protective Effect of Cyclosporine-A

    DOI: 10.1093/jbcr/irz029

    PMID: 30926991

    Sample: Serum
  79. Experimental and therapeutic medicine (2018) IF: 1.41
    Anti-inflammatory Effect of Resolvin D1 on LPS-treated MG-63 Cells

    DOI: 10.3892/etm.2018.6721

    PMID: 30402165

    Sample: Serum
  80. Experimental and Therapeutic Medicine (2019) IF: 1.41
    Effects of propofol combined with remifentanil anesthesia on the NO, endothelin and inflammatory cytokines in the plasma of patients with liver cirrhosis during the perioperative period

    DOI: 10.3892/etm.2019.7367

    PMID: 30988754

    Sample: Plasma
  81. Molecular Medicine Reports (2017) IF: 1.692
    Tetrahydroxy Stilbene Glucoside Ameliorates H2O2-Induced Human Brain Microvascular Endothelial Cell Dysfunction In Vitro By Inhibiting Oxidative Stress And Inflammatory Responses.

    DOI: 10.3892/mmr.2017.7225

    PMID: 28849141

    Sample: Cell culture supernatant
  82. Molecular Medicine Reports (2018) IF: 1.692
    The Shortening Telomere Length Of T Lymphocytes Maybe Associated With HyperFunction In Servere Aplastic Anemia.

    DOI: 10.3892/mmr.2017.8014

    PMID: 29115638

  83. Experimental and Therapeutic Medicine (2018) IF: 1.261
    Role Of miR-589-3p In Human Lumbar Disc Degeneration And Its Potential Mechanism.

    DOI: 10.3892/etm.2017.5593

    PMID: 29434746

    Sample: Cell culture supernatant
  84. Experimental and Therapeutic Medicine (2017) IF: 1.261
    Inhibition Of miR27a Suppresses The Inflammatory Response Via The p38/MAPK Pathway In Intervertebral Disc Cells.

    DOI: 10.3892/etm.2017.5053

    PMID: 29067127

    Sample: Cell culture supernatant
  85. The Journal of Gene Medicine (2020)
    Human cytomegalovirus infection is correlated with atherosclerotic plaque vulnerability in carotid artery

    DOI: 10.1002/jgm.3236

    PMID: 32468600

    Sample: Serum
  86. Int J Curr Microbiol App Sci (2015)
    The role of TNF and Resistin Gene+ 299 (G/A) polymorphism in the development of insulin resistance in non-obese Type 2 Diabetes Mellitus Iraqi patients
    Sample: Serum
  87. Biomedical and Pharmacology Journal (2016)
    Microbial Index and TNF-α, IL-4, CCL17 Level Among Burn Wound Infections in Hilla City, Iraq.

    DOI: 10.13005/bpj/1054

    Sample: Serum
  88. European Microscopy Congress 2016: Proceedings (2016)
    The activation, ROS induction and cytokine secretion in human monocytes exposed to H . pylori lipopolysaccharide depend on the availability of TLR4/2 and TLR2/6 receptor complexes

    DOI: 10.1002/9783527808465.EMC2016.6255

    Sample: Cell culture supernatant
  89. JMSCR (2018)
    Evaluation of Cytokines Response in Plasmodium falciparum Infection among Adolescents in Port Harcourt, Nigeria

    DOI: 10.18535/jmscr/v6i9.54

    Sample: Plasma
  90. Preprint (2019)
    The neuro-immune fingerprint of major neuro-cognitive psychosis or deficit schizophrenia: a supervised machine learning study

    DOI: 10.20944/preprints201905.0285.v1

    Sample: Serum
  91. Indian Journal of Forensic Medicine & Toxicology (2019)
    Influence of TLR4 Polymorphisms on Susceptibility to UTI Caused by Gram Negative Bacteria in Pregnant Women

    DOI: 10.5958/0973-9130.2019.00454.7

    Sample: Serum,Urine
  92. The Indonesian Biomedical Journal (2019)
    TNFR, TRAF2, NF-κB mRNA Levels of Glioblastoma Multiforme Cells Treated by Conditioned Medium of Umbilical Cord-derived Mesenchymal Stem Cells

    DOI: 10.18585/inabj.v11i2.722

    Sample: Cell lysate
  93. International Journal of Tropical Disease & Health (2019)
    Correlation of Small Dense Low Density Lipoprotein, Tumour Necrosis Factor-alpha with Liver Enzymes in Chronic Hepatitis B Patients

    DOI: 10.9734/ijtdh/2019/v37i430172

    Sample: Serum
  94. Indian Journal of Public Health (2020)
    Association of TLR4 Polymorphisms with Susceptibility to Urinary Tract Infection Caused by Gram Positive Bacteria in Pregnant Women
    Sample: Serum
  95. The Journal of Research on the Lepidoptera (2020)
    Estimation of IL-1βand TNF Concentration in Children Suffering from Febrile Convulsion

    DOI: 10.36872/LEPI/V51I2/301090

    Sample: Serum
  96. Preprint (2020)
    Thymosin β10 promotes tumor-associated macrophages M2 conversion and proliferation via the PI3K/Akt pathway in lung adenocarcinoma

    DOI: 10.21203/

    Sample: Cell culture supernatant
  97. The Journal of Immunology (2020)
    CALML6 Controls TAK1 Ubiquitination and Confers Protection against Acute Inflammation

    DOI: 10.4049/jimmunol.1901042

    PMID: 32303555

    Sample: Cell culture supernatant
  98. International Journal of Drug Delivery Technology (2020)
    Evaluation of Synergistic Effect of Cytosine-PhosphodiesterbondGuanosin-Oligodeoxynucleotide 7909, and Protamine on Transfection Process Mediated by Calcium Phosphate Nanoparticles
    Sample: Cell culture supernatant
  99. Preprints (2020)
    Lowered serum cesium levels in schizophrenia: association with immune-inflammatory biomarkers and cognitive impairments

    DOI: 10.1590/1516-4446-2020-0908

    PMID: 32556004

    Sample: Serum
  100. Annals of African Medicine (2019)
    Hyperhomocysteinemia and Folate Levels in Normal Healthy Nigerians Living in Zaria: Subanalysis of ABU Homocysteine Cross-sectional Survey

    DOI: 10.4103/aam.aam_53_18

    PMID: 31417015

    Sample: Plasma
  101. Revista da Associa??o Médica Brasileira (2019)
    TLR7 mediates increased vulnerability to ischemic acute kidney injury in diabetes

    DOI: 10.1590/1806-9282.65.8.1067

    PMID: 31531603

    Sample: Cell culture supernatant
  102. The Annals of thoracic surgery (2019)
    Impact of an Early Oral Feeding Protocol on Inflammatory Cytokine Changes After Esophagectomy

    DOI: 10.1016/j.athoracsur.2018.09.048

    PMID: 30403976

    Sample: Serum
  103. Modern Research in Inflammation (2019)
    Estimation of Cytokines Involved in Acute-Phase Wound Infection with Reference to Residence Time of Patients in Hospitals

    DOI: 10.4236/mri.2019.81001

    Sample: Serum
    Resveratrol protects human renal proximal tubular cells from high glucose and hypoxia/reoxygenation induced injury via inhibiting p38-MAPK and thioredoxin-interacting protein (TXNIP) pathways
    Sample: Cell culture supernatant
  105. Journal of Pharmaceutical Sciences and Research (2018)
    Effect of General and Spinal Anesthesia on Some Anti-inflammatory cytokines and cell adhesion molecules of Iraqi Women Undergo Cesarean Section: Comparative Study
    Sample: Serum
  106. Medical science monitor: international medical journal of experimental and clinical research (2018)
    Sinomenine Regulates Inflammatory Response and Oxidative Stress via Nuclear Factor kappa B (NF-kB) and NF-E2-Related Factor 2 (Nrf2) Signaling Pathways in Ankle Fractures in Children

    DOI: 10.12659/MSM.910740

    PMID: 30237391

    Sample: Serum
  107. University of Sulaimani (2018)
    Sample: Serum
  108. Medical Journal of Babylon (2017)
    Tumor Necrosis Factor Alpha Synergetic with Insulin Resistance Potentially Contributes to the Development of Polycystic Ovary Syndrome.
    Sample: Serum
    Potential Benefit of Melatonin as an Adjuvant Therapy in a Sample of Iraqi Patients with Crohn’s Disease: A New Study.
    Sample: Serum
  110. Kerbala Jorunal of Medicine (2017)
    Serum Tnfα Levels Are Inversely Associated With Insulin Sensitivity in Diabetic Retinopathy Patients.
    Sample: Serum
  111. Medical Science Monitor: International Medical Journal of Experimental And Clinical Research (2018) IF: /
    Propofol Promotes Ankle Fracture Healing in Children by Inhibiting Inflammatory Response.

    DOI: 10.12659/MSM.908592

    PMID: 29940605

    Sample: Serum
  112. Pediatric Allergy (2018) IF: 0.533
    High-Mobility Group Protein Box 1 is Upregulated in Children with Henoch-Schonlein Purpura.

    DOI: 10.1089/ped.2018.0872

    Sample: Serum
  113. Experimental and Therapeutic Medicine (2018)
    Leukocyte Depletion Does Not Affect The In Vitro Healing Ability Of Platelet Rich Plasma.

    DOI: 10.3892/etm.2018.5887

    PMID: 29556269

  114. Iraq Medical Journal (2018)
    Adiponectin may be used as a marker in prediction of diabetic retinopathy patients.
    Sample: Serum
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S*********nSubmitted [ Aug 05 2021 ]

Asked: My kit got expired in April 2020. Shall we perform the experiment with the kit?



adminSubmitted [ Aug 05 2021 ]

Answered: Thanks for your message. The expired kit can be used to perform experiment anymore. By the way, where did you ordered the kit from?

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