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Lactate Dehydrogenase (LDH) Cytotoxicity Colorimetric Assay Kit

Cat.No.:E-BC-K771-M
Detection instrument: Microplate reader (450 nm)

Detection method: Colorimetric method

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96T
500Assays

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Detection principle

Lactate dehydrogenase catalyzes the reaction of lactic acid with NAD+ to produce pyruvic acid and NADH. NADH, under the action of PMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, LDH activity can be quantified by measure the OD value at 450 nm.

Performance characteristics

Synonyms	LDH
Sample type	cells
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	30 min
Other instruments required	Microplate centrifuge, Micropipettor, Water bath
Storage	-20℃
Valid period	12 months

Images

Neither vancomycin nor D-11 affects the viability of human HEK-293 cells.

Q Li Lee et al investigate the function of outer-membrane-acting peptides in killing Gram-negative pathogens. Cytotoxicity on human cell was determined using lactate dehydrogenase (LDH) cytotoxicity colorimetric assay kit (E-BC-K771-M).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Communications Biology (2021) IF: 6.268

Outer-membrane-acting peptides and lipid II-targeting antibiotics cooperatively kill Gram-negative pathogens

DOI: 10.1038/s42003-020-01511-1
PMID: 33398076	Sample: Cell sample

CHEMICO-BIOLOGICAL INTERACTIONS (2021) IF: 5.194

In vitro and in vivo anticancer effects of syringic acid on colorectal cancer: Possible mechanistic view

DOI: 10.1016/j.cbi.2020.109337
PMID: 33548266	Sample: Cell sample

INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714

Activation of NLRP3 inflammasome contributes to the inflammatory response to allergic rhinitis via macrophage pyroptosis

DOI: 10.1016/j.intimp.2022.109012
PMID: 35809382	Sample: BMDMs

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018) IF: 3.575

Celastrol pretreatment attenuates rat myocardial ischemia/ reperfusion injury by inhibiting high mobility group box 1 protein expression via the PI3K/Akt pathway

DOI: 10.1016/j.bbrc.2018.02.121
	Sample: serum

MICROVASCULAR RESEARCH (2023) IF: 3.75

Co-administration of metformin and/or glibenclamide with losartan reverse NG-nitro-l-arginine-methyl ester-streptozotocin-induced hypertensive diabetes and haemodynamic sequelae in rats

DOI: 10.1016/j.mvr.2023.104497
	Sample: Serum

MOLECULAR BIOLOGY REPORTS (2023) IF: 2.742

Glioblastoma cell-derived exosomes induce cell death and oxidative stress in primary cultures of olfactory neurons. Role of redox stress

DOI: 10.1007/s11033-023-08256-0
	Sample: T98G cell lines,U373 cell lines

Biochemistry and Biophysics Reports (2022)

Simple neutralization test report: Do probiotics contribute to COVID-19 therapy?

DOI: 10.1016/j.bbrep.2022.101348
PMID: 36120494	Sample: PBMCs

Toxicology Reports (2019)

Elevated reproductive toxicity effects of diclofenac after withdrawal: Investigation of the therapeutic role of melatonin

DOI: 10.1016/j.toxrep.2019.06.009
	Sample: plasma

EGYPTIAN JOURNAL OF ANAESTHESIA (2022)

Analgesic efficacy, immunomodulation and complications of Erector spinae plane block in breast cancer surgeries: A randomized controlled trial

DOI: 10.1080/11101849.2022.2094070
	Sample: T cell

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