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Lactate Dehydrogenase (LDH) Cytotoxicity Colorimetric Assay Kit

  • Cat.No.:E-BC-K771-M

  • Detection instrument: Microplate reader (450 nm)

To Purchase E-BC-K771-M

Size:
  • 96T
  • 500Assays
Price: $140
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Detection principle

Lactate dehydrogenase catalyzes the reaction of lactic acid with NAD+ to produce pyruvic acid and NADH. NADH, under the action of PMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, LDH activity can be quantified by measure the OD value at 450 nm.

Performance characteristics

Synonyms LDH
Sample type cells
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 30 min
Other instruments required Microplate centrifuge, Micropipettor, Water bath
Storage -20℃
Valid period 12 months

Images

Neither vancomycin nor D-11 affects the viability of human HEK-293 cells.

Q Li Lee et al investigate the function of outer-membrane-acting peptides in killing Gram-negative pathogens. Cytotoxicity on human cell was determined using lactate dehydrogenase (LDH) cytotoxicity colorimetric assay kit (E-BC-K771-M).

Citations

  1. CHEMICO-BIOLOGICAL INTERACTIONS (2021) IF: 5.194
    In vitro and in vivo anticancer effects of syringic acid on colorectal cancer: Possible mechanistic view

    DOI: 10.1016/j.cbi.2020.109337

    PMID: 33548266

    Sample: Cell Sample
  2. Biochemistry and Biophysics Reports (2022)
    Simple neutralization test report: Do probiotics contribute to COVID-19 therapy?

    DOI: 10.1016/j.bbrep.2022.101348

    PMID: 36120494

    Sample: Pbmcs
  3. Toxicology Reports (2019)
    Elevated reproductive toxicity effects of diclofenac after withdrawal: Investigation of the therapeutic role of melatonin

    DOI: 10.1016/j.toxrep.2019.06.009

    Sample: Plasma
  4. EGYPTIAN JOURNAL OF ANAESTHESIA (2022)
    Analgesic efficacy, immunomodulation and complications of Erector spinae plane block in breast cancer surgeries: A randomized controlled trial

    DOI: 10.1080/11101849.2022.2094070

    Sample: T Cell
  5. MOLECULAR BIOLOGY REPORTS (2023) IF: 2.742
    Glioblastoma cell-derived exosomes induce cell death and oxidative stress in primary cultures of olfactory neurons. Role of redox stress

    DOI: 10.1007/s11033-023-08256-0

    Sample: T98G Cell Lines,U373 Cell Lines
  6. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018) IF: 3.575
    Celastrol pretreatment attenuates rat myocardial ischemia/ reperfusion injury by inhibiting high mobility group box 1 protein expression via the PI3K/Akt pathway

    DOI: 10.1016/j.bbrc.2018.02.121

    Sample: Serum
  7. MICROVASCULAR RESEARCH (2023) IF: 3.75
    Co-administration of metformin and/or glibenclamide with losartan reverse NG-nitro-l-arginine-methyl ester-streptozotocin-induced hypertensive diabetes and haemodynamic sequelae in rats

    DOI: 10.1016/j.mvr.2023.104497

    Sample: Serum
  8. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
    Activation of NLRP3 inflammasome contributes to the inflammatory response to allergic rhinitis via macrophage pyroptosis

    DOI: 10.1016/j.intimp.2022.109012

    PMID: 35809382

    Sample: Bmdms
  9. Communications Biology (2021) IF: 6.268
    Outer-membrane-acting peptides and lipid II-targeting antibiotics cooperatively kill Gram-negative pathogens

    DOI: 10.1038/s42003-020-01511-1

    PMID: 33398076

    Sample: Cell Sample
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