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Lipid Peroxide (LPO) Colorimetric Assay Kit

Cat.No.:E-BC-K176-M
Detection instrument: Microplate reader(580-590 nm)

Detection method: Colorimetric method

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Detection principle

With 45℃ incubation for 60 min, one molecule of LPO react with two molecule of chromogenic reagent, to produce a stable chromophore which have the maximum absorption peak at 586nm. The content of LPO in samples can be calculated by standard curve or calculation formula.

Performance characteristics

Synonyms	LPO
Sample type	Serum,plasma,urine,tissue
Sensitivity	0.70 μmol/L
Detection range	0.70-80 μmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	90 min
Precision	Average inter-assay CV: 3.5%Average intra-assay CV: 3.1%
Other instruments required	Water bath, Centrifuge, Micropipettor, Vortex mixer
Other reagents required	Double distilled water, PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range ( 0.70-80 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human plasma	1
Urine	1
10% Rat kidney tissue homogenization	1
10% Rat liver tissue homogenization	1

Note: The diluent is PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

Indian Journal of Forensic Medicine & Toxicology (2020)

Reduced Glutathione, Lipid Peroxidation and Malondialdehyde Status in Women with Mild and Severe Preeclampsia for Babylon Governorate

DOI: 10.37506/ijfmt.v14i1.144
	Sample: Serum

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