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Current Position:Home>>Kits>>Metabolism Assays>> Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)

Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)

Citations(84)

  • Cat.No.:E-BC-K025-M

  • Detection instrument: Microplate reader(530-540 nm,optimum wavelength: 532 nm)

Manual MSDS

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  • 96T
  • 48T
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Detection principle

MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.

Performance characteristics

Synonyms MDA
Sample type Serum,plasma,animal tissue
Sensitivity 1.13 μmol/L
Detection range 2.92-40 μmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 65 min
Precision Average inter-assay CV: 7.200%Average intra-assay CV: 4.100%
Other instruments required Micropipettor, Vortex mixer, Incubator, Centrifuge, Magnetic Stirrers
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol
Storage 2-8℃
Valid period 12 months

Images

The level of MDA was significantly lower (P<0.001) in NPCoQ10 groups and even lower in N-NPCoQ10 group.

Z Liu et al found that the usage of N-NPCoQ10 nano-particle can help for transforming the antioxidants which could ameliorate the renal I-R injury in mice. Malondialdehyde (MDA) of rat serum was determined using MDA colorimetric assay kit (E-BC-K025-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the formal experiment and the detection range (2.92-40 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Human plasma

1

Rat serum

1

Rat plasma

1

Mouse serum

1

Mouse plasma

1

10% Rat heart tissue homogenate

1

10% Rat liver tissue homogenate

1

10% Rat spleen tissue homogenate

1

10% Rat lung tissue homogenate

1

10% Rat kidney tissue homogenate

1

10% Rat brain tissue homogenate

1


Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


Citations

  1. FOOD AND CHEMICAL TOXICOLOGY (2022) IF: 6.025
    Procyanidins inhibit zearalenone-induced apoptosis and oxidative stress of porcine testis cells through activation of Nrf2 signaling pathway

    DOI: 10.1016/j.fct.2022.113061

    PMID: 35489465

    		 Sample: Pig Testicular Cell (St Cell)
  2. FOOD AND CHEMICAL TOXICOLOGY (2021) IF: 6.025
    Protective effect of alpha-lipoic acid on bisphenol A-induced learning and memory impairment in developing mice: nNOS and keap1/Nrf2 pathway

    DOI: 10.1016/j.fct.2021.112307

    PMID: 34058234

    		 Sample: Serum
  3. Cancers (2023) IF: 6.575
    CircZBTB46 Protects Acute Myeloid Leukemia Cells from Ferroptotic Cell Death by Upregulating SCD

    DOI: 10.3390/cancers15020459
    Sample: Acute Myeloid Leukemia (Aml) Cell
  4. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Comparison of Selenium Nanoparticles and Sodium Selenite on the Alleviation of Early Atherosclerosis by Inhibiting Endothelial Dysfunction and Inflammation in Apolipoprotein E-Deficient Mice

    DOI: 10.3390/ijms222111612

    PMID: 34769040

    		 Sample: Serum
  5. Pharmaceuticals (2021) IF: 5.863
    Fucoidan Protects against Acute Sulfoxaflor-Induced Hematological/Biochemical Alterations and Oxidative Stress in Male Mice

    DOI: 10.3390/ph15010016
    Sample: Serum
  6. FOOD AND CHEMICAL TOXICOLOGY (2021) IF: 6.025
    Bacillus amyloliquefaciens B10 can alleviate liver apoptosis and oxidative stress induced by aflatoxin B1

    DOI: 10.1016/j.fct.2021.112124

    PMID: 33727180

    		 Sample: Tissue Homogenate
  7. Toxicology Reports (2023)
    Interactions of alcohol and combination antiretroviral (cART) drug in diabetic male Sprague Dawley rats: Hippocampal perturbations and toxicosis

    DOI: 10.1016/j.toxrep.2023.01.009
    Sample: Brain
  8. Toxicology Reports (2019)
    Elevated reproductive toxicity effects of diclofenac after withdrawal: Investigation of the therapeutic role of melatonin

    DOI: 10.1016/j.toxrep.2019.06.009
    Sample: Plasma
  9. Veterinary World (2020)
    Cinnamomum burmannii (Nees & T. Nees) Blume and Eleutherine palmifolia (L.) Merr. extract combination ameliorate lipid profile and heart oxidative stress in hyperlipidemic mice

    DOI: 10.14202/vetworld.2020.1404-1409

    PMID: 32848317

    		 Sample: Serum
  10. BIOLOGICAL RHYTHM RESEARCH (2022) IF: 1.219
    Impact of photoperiods on the specific activities of immune and antioxidant enzymes in different tissues of Dybowski’s frog (Rana dybowskii)

    DOI: 10.1080/09291016.2022.2043588
    Sample: Serum,Liver,Skin
  11. JOURNAL OF OCCUPATIONAL AND ENVIRONMENTAL MEDICINE (2020) IF: 1.642
    Spirometric Outcomes and Oxidative Stress Among Cement Factory Workers: Results From a Cross-Sectional Study

    DOI: 10.1097/JOM.0000000000001991

    PMID: 32796257

    		 Sample: Serum
  12. DRUG DEVELOPMENT RESEARCH (2019) IF: 1.742
    Montelukast modifies simvastatin‐induced myopathy and hepatotoxicity

    DOI: 10.1002/ddr.21581
    Sample: Liver
  13. VETERINARY RESEARCH COMMUNICATIONS (2022) IF: 2.459
    Infection of West African dwarf rams with Trypanosoma brucei brucei and Trypanosoma congolense significantly alter serum electrolytes, redox balance, sperm parameters, and gonadal morphology

    DOI: 10.1007/s11259-022-09921-8

    PMID: 35389159

    		 Sample: Serum
  14. Translational Andrology and Urology (2022) IF: 2.479
    Astragaloside IV protects against ischemia/reperfusion (I/R)-induced kidney injury based on the Keap1-Nrf2/ARE signaling pathway.

    DOI: 10.21037/tau-22-505
    Sample: Kidney Tissue
  15. Dose-Response (2022) IF: 2.623
    Pharmacological Role of Capparis decidua (Forssk.) Edgew in Preventing Cyclophosphamide-induced Myelosuppression and Modulating Innate and Adaptive Immune Response:

    DOI: 10.1177/15593258221123672
    Sample: Serum
  16. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    Zanthoxylum bungeanum Seed Oil Attenuates LPS-Induced BEAS-2B Cell Activation and Inflammation by Inhibiting the TLR4/MyD88/NF-κB Signaling Pathway

    DOI: 10.1155/2021/2073296
    Sample: Beas-2B Cell
  17. Sleep and Breathing (2023) IF: 2.655
    The role of ferroptosis in chronic intermittent hypoxia-induced cognitive impairment

    DOI: 10.1007/s11325-022-02760-6
    Sample: Hippocampal Tissue
  18. Journal of Stroke & Cerebrovascular Diseases (2022) IF: 2.677
    Circ_0101874 overexpression strengthens PDE4D expression by targeting miR-335-5p to promote neuronal injury in ischemic stroke

    DOI: 10.1016/j.jstrokecerebrovasdis.2022.106817

    PMID: 36252429

    		 Sample: Sk-N-Sh Cells
  19. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 2.72
    Effect of coconut water and milk on heat stress-induced gastrointestinal tract dysmotility in rats: Role of oxidative stress and inflammatory response

    DOI: 10.1111/jfbc.14129

    PMID: 35298033

    		 Sample: Gastric,Jejunal, Colonic
  20. JOURNAL OF FOOD BIOCHEMISTRY (2021) IF: 2.72
    A standardized black pepper seed extract containing β-caryophyllene improves cognitive function in scopolamine-induced amnesia model mice via regulation of brain-derived neurotrophic factor and MAPK proteins

    DOI: 10.1111/jfbc.13994

    PMID: 34778972

    		 Sample: Brain
  21. FUNDAMENTAL & CLINICAL PHARMACOLOGY (2022) IF: 2.747
    The effect of diminazene, an angiotensin-converting enzyme 2 activator, on adenine-induced chronic kidney disease in rats

    DOI: 10.1111/fcp.12845
    Sample: Renal
  22. CURRENT ISSUES IN MOLECULAR BIOLOGY (2022) IF: 2.976
    Increased Levels of Autoantibodies against ROS-Modified Proteins in Depressed Individuals with Decrease in Antibodies against SARS-CoV-2 Antigen (S1-RBD)

    DOI: 10.3390/cimb44110358

    PMID: 36354670

    		 Sample: Serum
  23. CURRENT ISSUES IN MOLECULAR BIOLOGY (2023) IF: 2.976
    Chemopreventive Effects of Onosma mutabilis against Azoxymethane-Induced Colon Cancer in Rats via Amendment of Bax/Bcl-2 and NF-κB Signaling Pathways

    DOI: 10.3390/cimb45020057
    Sample: Colonic Tissue
  24. Reproductive Sciences (2021) IF: 3.06
    Impact of Melatonin on Full-Term Fetal Brain Development and Transforming Growth Factor-β Level in a Rat Model of Preeclampsia

    DOI: 10.1007/s43032-021-00497-3

    PMID: 33591564

    		 Sample: Tissue Homogenate( Placental,Fetal Brain)
  25. Frontiers in Bioscience-Landmark (2022) IF: 3.115
    SIRT3 Enhances the Protective Role of Propofol in Postoperative Cognitive Dysfunction via Activating Autophagy Mediated by AMPK/mTOR Pathway

    DOI: 10.31083/j.fbl2711303
    Sample: Hippocampal Tissue,Primary Neurons
  26. Animals (2023) IF: 3.231
    The Effects of Dietary Spirulina platensisis on Physiological Responses of Broiler Chickens Exposed to Endotoxin Stress

    DOI: 10.3390/ani13030363
    Sample: Plasma
  27. Biomed Research International (2022) IF: 3.246
    Red Raspberry Extract Decreases Depression-Like Behavior in Rats by Modulating Neuroinflammation and Oxidative Stress

    DOI: 10.1155/2022/9943598

    PMID: 35818443

    		 Sample: Hippocampal Tissue
  28. Processes (2022) IF: 3.352
    Gastroprotective, Biochemical, and Acute Toxicity Effects of Papaver decaisnei against Ethanol-Induced Gastric Ulcers in Rats

    DOI: 10.3390/pr10101985
    Sample: Gastric Tissue
  29. Processes (2022) IF: 3.352
    Gastroprophylactic Effects of p-Cymene in Ethanol-Induced Gastric Ulcer in Rats

    DOI: 10.3390/pr10071314
    Sample: Stomach
  30. Frontiers in Veterinary Science (2022) IF: 3.412
    Ovarian Oxidative Stress Induced Follicle Depletion After Zona Pellucida 3 Vaccination Is Associated With Subfertility in BALB/c Mice

    DOI: 10.3389/fvets.2022.814827

    PMID: 35252419

    		 Sample: Ovarian
  31. PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
    Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

    DOI: 10.1080/13880209.2022.2070221

    PMID: 35635784

    		 Sample: Brain Tissue
  32. Journal of Personalized Medicine (2022) IF: 3.508
    Artichoke (Cynara Scolymus) Methanolic Leaf Extract Alleviates Diethylnitrosamine-Induced Toxicity in BALB/c Mouse Brain: Involvement of Oxidative Stress and Apoptotically Related Klotho/PPARγ Signaling

    DOI: 10.3390/jpm12122012
    Sample: Brain
  33. Porcine Health Management (2022) IF: 3.535
    Is a new generation of mycotoxin clay adsorbents safe in a pig’s diet?

    DOI: 10.1186/s40813-022-00275-w
    Sample: Blood
  34. METABOLIC BRAIN DISEASE (2022) IF: 3.584
    LncRNA ZFAS1 regulates the hippocampal neurons injury in epilepsy through the miR-15a-5p/OXSR1/NF-κB pathway

    DOI: 10.1007/s11011-022-01013-5

    PMID: 35751788

    		 Sample: Hippocampal Neurons
  35. LIFE SCIENCES (2020) IF: 3.647
    Quercetin attenuated oxidative DNA damage through NRF2 signaling pathway in rats with DMH induced colon carcinogenesis

    DOI: 10.1016/j.lfs.2020.117584

    PMID: 32220623

    		 Sample: Tissue Homogenate
  36. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 3.654
    Viphyllin™, a standardized extract from black pepper seeds, mitigates intestinal inflammation, oxidative stress, and anxiety-like behavior in DSS-induced colitis mice

    DOI: 10.1111/jfbc.14306

    PMID: 35766031

    		 Sample: Colon Tissue,Brain Tissue
  37. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 3.654
    Twelve weeks of treadmill exercise training with green tea extract reduces myocardial oxidative stress and alleviates cardiomyocyte apoptosis in aging rat: The emerging role of BNIP3 and HIF-1α/IGFBP3 pathway

    DOI: 10.1111/jfbc.14397

    PMID: 36069470

    		 Sample: Heart Tissue
  38. Diagnostics (2022) IF: 3.706
    Combination Antiretroviral Therapy (cART) in Diabetes Exacerbates Diabetogenic Effects on Hippocampal Microstructure, Neurogenesis and Cytokine Perturbation in Male Sprague Dawley Rats

    DOI: 10.3390/diagnostics12040905

    PMID: 35453953

    		 Sample: Brain
  39. BRAIN RESEARCH BULLETIN (2022) IF: 3.715
    Chuanzhitongluo regulates microglia polarization and inflammatory response in acute ischemic stroke

    DOI: 10.1016/j.brainresbull.2022.09.015

    PMID: 36152772

    		 Sample: Brain Tissue
  40. AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2022) IF: 3.886
    Fetal hyperhomocysteinemia is associated with placental inflammation and early breakdown of maternal-fetal tolerance in Pre-term birth

    DOI: 10.1111/aji.13589

    PMID: 35750632

    		 Sample: Serum
  41. PHARMACEUTICAL BIOLOGY (2023) IF: 3.889
    Nanocurcumin preserves kidney function and haematology parameters in DMBA-induced ovarian cancer treated with cisplatin via its antioxidative and anti-inflammatory effect in rats

    DOI: 10.1080/13880209.2023.2166965
    Sample: Plasma
  42. Annals of Translational Medicine (2022) IF: 3.932
    Levosimendan protects from sepsis-inducing cardiac dysfunction by suppressing inflammation, oxidative stress and regulating cardiac mitophagy via the PINK-1-Parkin pathway in mice

    DOI: 10.21037/atm-22-483

    PMID: 35280364

    		 Sample: Myocardium
  43. INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 3.943
    Astragaloside IV alleviates PM2.5-induced lung injury in rats by modulating TLR4/MyD88/NF-κB signalling pathway

    DOI: 10.1016/j.intimp.2020.107290

    PMID: 33383446

    		 Sample: Lung Tissue
  44. CELLULAR SIGNALLING (2020) IF: 3.968
    Circular RNA circ_DROSHA alleviates the neural damage in a cell model of temporal lobe epilepsy through regulating miR-106b-5p/MEF2C axis

    DOI: 10.1016/j.cellsig.2020.109901

    PMID: 33370579

    		 Sample: Sk-N-Sh Cell,Imr-32 Cell
  45. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY (2021) IF: 3.981
    Using PCG-Arginine nanoparticle mediated intranasal delivery of dynorphin A (1–8) to improve neuroprotection in MCAO rats

    DOI: 10.1016/j.jddst.2021.103059
    Sample: Serum
  46. Scientific Reports (2020) IF: 3.998
    Quercetin liposomes ameliorate streptozotocin-induced diabetic nephropathy in diabetic rats

    DOI: 10.1038/s41598-020-59411-7
    Sample: Kidney Tissue
  47. TOXICOLOGY (2020) IF: 4.099
    Inhibition of ferroptosis attenuates busulfan-induced oligospermia in mice

    DOI: 10.1016/j.tox.2020.152489
    Sample: Testicular Tissue
  48. AQUACULTURE (2021) IF: 4.242
    Synergistic toxicity of dietary aflatoxin B1 (AFB1) and zearalenone (ZEN) in rainbow trout (Oncorhynchus mykiss) is attenuated by anabolic effects

    DOI: 10.1016/j.aquaculture.2021.736793
    Sample: Serum
  49. Foods (2022) IF: 4.35
    Protective Effects of a Novel Probiotic Bifidobacterium pseudolongum on the Intestinal Barrier of Colitis Mice via Modulating the Pparγ/STAT3 Pathway and Intestinal Microbiota

    DOI: 10.3390/foods11111551
    Sample: Colon Tissue
  50. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Sub-chronic oral toxicity evaluation of herbo-metallic formulation Arshakuthar rasa in rats

    DOI: 10.1016/j.jep.2022.115306

    PMID: 35443217

    		 Sample: Serum
  51. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Anti-inflammatory and anti-apoptotic effects of Shaoyao decoction on X-ray radiation-induced enteritis of C57BL/6 mice

    DOI: 10.1016/j.jep.2022.115158

    PMID: 35245630

    		 Sample: Serum
  52. Scientific Reports (2021) IF: 4.38
    Effect of tiger milk mushroom (Lignosus rhinocerus) supplementation on respiratory health, immunity and antioxidant status: an open-label prospective study

    DOI: 10.1038/s41598-021-91256-6

    PMID: 34083710

    		 Sample: Saliva
  53. Scientific Reports (2021) IF: 4.38
    2D exfoliated black phosphorus influences healthy and cancer prostate cell behaviors

    DOI: 10.1038/s41598-021-85310-6

    PMID: 33712665

    		 Sample: Cell Culture Supernatant
  54. MOLECULES (2021) IF: 4.412
    Insight into Seasonal Change of Phytochemicals, Antioxidant, and Anti-Aging Activities of Root Bark of Paeonia suffruticosa (Cortex Moutan) Combined with Multivariate Statistical Analysis

    DOI: 10.3390/molecules26206102

    PMID: 34684685

    		 Sample: Hdf Cell
  55. Frontiers in Genetics (2022) IF: 4.599
    Presence of Circulatory Autoantibodies Against ROS-Modified Histone H1 Protein in Lymphoma Patients.

    DOI: 10.3389/fgene.2022.909903
    Sample: Serum
  56. PROCESS BIOCHEMISTRY (2022) IF: 4.885
    Vernodalin regulated the NF-κβp65 signaling in inflammation of lipopolysaccharide -induced sepsis rats

    DOI: 10.1016/j.procbio.2022.10.004
    Sample: Liver,Kidney,Lungs
  57. MOLECULES (2022) IF: 4.927
    Chemical Profile of Cyperus laevigatus and Its Protective Effects against Thioacetamide-Induced Hepatorenal Toxicity in Rats

    DOI: 10.3390/molecules27196470

    PMID: 36235007

    		 Sample: Plasma
  58. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
    Gastroprotective effect of an active ingredients group of Lindera reflexa Hemsl. On Ethanol-Induced gastric ulcers in Rats: Involvement of VEGFR2/ERK and TLR-2/Myd88 signaling pathway

    DOI: 10.1016/j.intimp.2022.108673

    PMID: 35259712

    		 Sample: Stomach Tissues
  59. Scientific Reports (2022) IF: 4.996
    Effects of yoga on cardiometabolic risks and fetomaternal outcomes are associated with serum nitric oxide in gestational hypertension: a randomized control trial

    DOI: 10.1038/s41598-022-15216-4

    PMID: 35821033

    		 Sample: Serum
  60. Antioxidants (2020) IF: 5.014
    Dietary Postbiotic Lactobacillus plantarum Improves Serum and Ruminal Antioxidant Activity and Upregulates Hepatic Antioxidant Enzymes and Ruminal Barrier Function in Post-Weaning Lambs

    DOI: 10.3390/antiox9030250
    Sample: Serum,Ruminal Fluid
  61. Food Bioscience (2022) IF: 5.318
    Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice

    DOI: 10.1016/j.fbio.2022.102079
    Sample: Livers
  62. Foods (2022) IF: 5.561
    Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota

    DOI: 10.3390/foods11193048

    PMID: 36230124

    		 Sample: Serum
  63. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
    The role of AMPK-Sirt1-autophagy pathway in the intestinal protection process by propofol against regional ischemia/reperfusion injury in rats

    DOI: 10.1016/j.intimp.2022.109114

    PMID: 35933747

    		 Sample: Intestinal Tissue,Serum
  64. Frontiers in Oncology (2022) IF: 5.738
    ACO1 and IREB2 downregulation confer poor prognosis and correlate with autophagy-related ferroptosis and immune infiltration in KIRC.

    DOI: 10.3389/fonc.2022.929838
    Sample: Achn Cell
  65. Frontiers in Pharmacology (2021) IF: 5.811
    Identification of the Antithrombotic Mechanism of Leonurine in Adrenalin Hydrochloride-Induced Thrombosis in Zebrafish via Regulating Oxidative Stress and Coagulation Cascade.

    DOI: 10.3389/fphar.2021.742954
  66. Frontiers in Pharmacology (2022) IF: 5.988
    Mailuoshutong pill for varicocele-associated male infertility-Phytochemical characterisation and multitarget mechanism.

    DOI: 10.3389/fphar.2022.961011
    Sample: Testicular Tissue
  67. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    An Association between Insulin Resistance and Neurodegeneration in Zebrafish Larval Model (Danio rerio)

    DOI: 10.3390/ijms23158290
    Sample: Embryo
  68. Antioxidants (2021) IF: 6.313
    N-Acetyl Cysteine Overdose Inducing Hepatic Steatosis and Systemic Inflammation in Both Propacetamol-Induced Hepatotoxic and Normal Mice

    DOI: 10.3390/antiox10030442

    PMID: 33809388

    		 Sample: Tissue Homogenate
  69. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    Preventing the onset of diabetes-induced chronic kidney disease during prediabetes: The effects of oleanolic acid on selected markers of chronic kidney disease in a diet-induced prediabetic rat model

    DOI: 10.1016/j.biopha.2021.111570

    PMID: 33932738

    		 Sample: Tissue Homogenate(Kidney)
  70. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    Lipoic acid (LA) dose-dependently protects bone losses in the mandible of rats during the development of osteopenia by inhibiting oxidative stress and promoting bone formation

    DOI: 10.1016/j.biopha.2021.112467

    PMID: 34891114

    		 Sample: Serum
  71. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
    Daming capsule protects against myocardial infarction by promoting mitophagy via the SIRT1/AMPK signaling pathway

    DOI: 10.1016/j.biopha.2022.113162

    PMID: 35676781

    		 Sample: Serum
  72. Oxidative Medicine and Cellular Longevity (2021) IF: 6.543
    Leonurine Ameliorates Oxidative Stress and Insufficient Angiogenesis by Regulating the PI3K/Akt-eNOS Signaling Pathway in H2O2-Induced HUVECs

    DOI: 10.1155/2021/9919466
    Sample: Huvecs
  73. INDOOR AIR (2022) IF: 6.554
    Organic pollutant exposure and health effects of cooking emissions on kitchen staff in food services

    DOI: 10.1111/ina.13093

    PMID: 36040287

    		 Sample: Serum
  74. LIFE SCIENCES (2022) IF: 6.78
    A bovine milk-derived peptide ameliorates alloxan-injured pancreatic β cells through IRS2/PI3K/Akt signaling

    DOI: 10.1016/j.lfs.2022.120907

    PMID: 36037870

    		 Sample: Pancreatic Tissue
  75. Acta Biomaterialia (2020) IF: 7.242
    Neutrophil membrane-enveloped nanoparticles for the amelioration of renal ischemia-reperfusion injury in mice

    DOI: 10.1016/j.actbio.2020.01.018

    PMID: 31954188

    		 Sample: Serum
  76. Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
    Tetramethylpyrazine Protects Endothelial Injury and Antithrombosis via Antioxidant and Antiapoptosis in HUVECs and Zebrafish

    DOI: 10.1155/2022/2232365

    PMID: 35898617

    		 Sample: Huvecs
  77. Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
    Mesenchymal Stem Cell-Derived Exosomes Ameliorate Delayed Neurocognitive Recovery in Aged Mice by Inhibiting Hippocampus Ferroptosis via Activating SIRT1/Nrf2/HO-1 Signaling Pathway

    DOI: 10.1155/2022/3593294

    PMID: 36238648

    		 Sample: Hippocampus
  78. Antioxidants (2022) IF: 7.675
    Selenoprotein F Knockout Caused Glucose Metabolism Disorder in Young Mice by Disrupting Redox Homeostasis

    DOI: 10.3390/antiox11112105

    PMID: 36358477

    		 Sample: Liver,Serum
  79. Antioxidants (2022) IF: 7.675
    Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats

    DOI: 10.3390/antiox11101879

    PMID: 36290602

    		 Sample: Ovarian Tissue
  80. Antioxidants (2022) IF: 7.675
    Effects of Gold Nanoparticles Functionalized with Bioactive Compounds from Cornus mas Fruit on Aorta Ultrastructural and Biochemical Changes in Rats on a Hyperlipid Diet—A Preliminary Study

    DOI: 10.3390/antiox11071343
    Sample: Serum,Aorta
  81. SCIENCE OF THE TOTAL ENVIRONMENT (2021) IF: 7.963
    Polystyrene microplastics induce hepatotoxicity and disrupt lipid metabolism in the liver organoids

    DOI: 10.1016/j.scitotenv.2021.150328

    PMID: 34571217

    		 Sample: Liver Organoids (Los)
  82. FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 8.101
    miR-495–3p regulates sphingolipid metabolic reprogramming to induce Sphk1/ceramide mediated mitophagy and apoptosis in NSCLC

    DOI: 10.1016/j.freeradbiomed.2022.07.001
    Sample: A549 Cell,H1299 Cell
  83. Journal of Neuroinflammation (2021) IF: 8.322
    Sirtuin 3 protects against anesthesia/surgery-induced cognitive decline in aged mice by suppressing hippocampal neuroinflammation

    DOI: 10.1186/s12974-021-02089-z

    PMID: 33541361

    		 Sample: Tissue Homogenate(Hippocampal Homogenate)
  84. CELLULAR & MOLECULAR BIOLOGY LETTERS (2023) IF: 8.702
    Formyl peptide receptor 2 activation by mitochondrial formyl peptides stimulates the neutrophil proinflammatory response via the ERK pathway and exacerbates ischemia–reperfusion injury

    DOI: 10.1186/s11658-023-00416-1
    Sample: Kidney Tissue
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Reviews/Q&A

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  • Reviews
  • Q&A
Q

E*** Submitted [ May 20 2024 ]

Asked: Hi, may I know what\'s the difference between this TBA Method MDA Colorimetric Assay Kit and the Cell Samples MDA Colorimetric Assay Kit? Can I use this TBA Method MDA assay kit to measure MDA in cell lysate?

Reply

A

adminSubmitted [ May 20 2024 ]

Answered: Hello, are you inquiring about E-BC-K025-M kit and E-BC-K028-M kit? The reagent composition, sensitivity, sample preparation and operating steps of the two kits are different. E-BC-K025-M kit can not be used for the detection of cell samples, suitable for the detection of serum (plasma), tissue samples. E-BC-K028-M kit is specifically used for the detection of cell samples. It is recommended that you select the corresponding item number according to the sample type.

Q

S**********rSubmitted [ Mar 05 2024 ]

Asked: For rat brain samples, should we prepare control tube? Or should control tubes be prepared only for hemolyzed or lipidemia serum samples? Also should control tubes be prepared for all samples? If so, can we study only 40 samples with one assay kit?

Reply

A

adminSubmitted [ Mar 05 2024 ]

Answered: The E-BC-K025-M kit is set for control only for serum or plasma samples, that is, if there is hemolysis or lipemia, in order to exclude the interference of the sample itself, each sample needs to do a sample tube and a control. If you are detecting tissue samples, you do not need to set up the control. The kit of 96T specification can detect 80 samples if the samples are not repeated.

Q

M*****aSubmitted [ Oct 14 2022 ]

Asked: Dear, does this kit have 10T specification?

Reply

A

adminSubmitted [ Oct 14 2022 ]

Answered: We cannot provide 10T size, and the specification minimum of biochemical kit is 48T.

Q

M*******aSubmitted [ Oct 09 2022 ]

Asked: Can i use this to measure MDA in cell lysate?

Reply

A

adminSubmitted [ Oct 09 2022 ]

Answered: Hi Marchella, I'll reply you by mail. sincerely Amanda

Q

M*****aSubmitted [ Sep 14 2022 ]

Asked: oxidative index in fish blood/serum: MDA?

Reply

A

adminSubmitted [ Sep 14 2022 ]

Answered: It can be detect the fish blood.

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