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Cat.No.:E-BC-K025-M
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Synonyms | MDA |
Sample type | Serum,plasma,animal tissue |
Sensitivity | 1.13 μmol/L |
Detection range | 2.92-40 μmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 65 min |
Precision | Average inter-assay CV: 7.200%Average intra-assay CV: 4.100% |
Other instruments required | Micropipettor, Vortex mixer, Incubator, Centrifuge, Magnetic Stirrers |
Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol |
Storage | 2-8℃ |
Valid period | 12 months |
The level of MDA was significantly lower (P<0.001) in NPCoQ10 groups and even lower in N-NPCoQ10 group.
Z Liu et al found that the usage of N-NPCoQ10 nano-particle can help for transforming the antioxidants which could ameliorate the renal I-R injury in mice. Malondialdehyde (MDA) of rat serum was determined using MDA colorimetric assay kit (E-BC-K025-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the formal experiment and the detection range (2.92-40 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
1 |
Human plasma |
1 |
Rat serum |
1 |
Rat plasma |
1 |
Mouse serum |
1 |
Mouse plasma |
1 |
10% Rat heart tissue homogenate |
1 |
10% Rat liver tissue homogenate |
1 |
10% Rat spleen tissue homogenate |
1 |
10% Rat lung tissue homogenate |
1 |
10% Rat kidney tissue homogenate |
1 |
10% Rat brain tissue homogenate |
1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Sample: A549 cell,H1299 cell |
PMID: 33541361 |
Sample: Tissue homogenate(Hippocampal homogenate) |
Sample: Kidney tissue |
PMID: 31954188 |
Sample: Serum |
DOI: 10.1155/2022/2232365 |
|
PMID: 35898617 |
Sample: HUVECs |
DOI: 10.1155/2022/3593294 |
|
PMID: 36238648 |
Sample: hippocampus |
PMID: 36358477 |
Sample: liver,serum |
PMID: 36290602 |
Sample: ovarian tissue |
Sample: serum,aorta |
PMID: 34571217 |
Sample: liver organoids (LOs) |
PMID: 34058234 |
Sample: Serum |
PMID: 35489465 |
Sample: pig testicular cell (ST cell) |
PMID: 33727180 |
Sample: Tissue homogenate |
DOI: 10.3390/ijms23158290 |
|
Sample: embryo |
PMID: 33809388 |
Sample: Tissue homogenate |
PMID: 33932738 |
Sample: Tissue homogenate(kidney) |
PMID: 34891114 |
Sample: serum |
PMID: 35676781 |
Sample: serum |
DOI: 10.1155/2021/9919466 |
|
Sample: HUVECs |
DOI: 10.1111/ina.13093 |
|
PMID: 36040287 |
Sample: serum |
Sample: acute myeloid leukemia (AML) cell |
PMID: 36037870 |
Sample: pancreatic tissue |
Sample: serum,ruminal fluid |
Sample: livers |
PMID: 36230124 |
Sample: serum |
PMID: 35933747 |
Sample: intestinal tissue,serum |
Sample: ACHN cell |
DOI: 10.3390/ph15010016 |
|
Sample: serum |
PMID: 34769040 |
Sample: serum |
Sample: testicular tissue |
Sample: testicular tissue |
Sample: Serum |
Sample: colon tissue |
PMID: 35443217 |
Sample: serum |
PMID: 35245630 |
Sample: Serum |
PMID: 34083710 |
Sample: saliva |
PMID: 33712665 |
Sample: Cell culture supernatant |
PMID: 34684685 |
Sample: HDF cell |
Sample: serum |
Sample: liver,kidney,lungs |
PMID: 36235007 |
Sample: plasma |
PMID: 35259712 |
Sample: stomach tissues |
PMID: 35821033 |
Sample: serum |
PMID: 33591564 |
Sample: Tissue homogenate( placental,fetal brain) |
Sample: hippocampal tissue,Primary Neurons |
DOI: 10.3390/ani13030363 |
|
Sample: Plasma |
DOI: 10.1155/2022/9943598 |
|
PMID: 35818443 |
Sample: hippocampal tissue |
DOI: 10.3390/pr10101985 |
|
Sample: gastric tissue |
DOI: 10.3390/pr10071314 |
|
Sample: stomach |
PMID: 35252419 |
Sample: Ovarian |
PMID: 35635784 |
Sample: brain tissue |
DOI: 10.3390/jpm12122012 |
|
Sample: brain |
Sample: blood |
PMID: 35751788 |
Sample: hippocampal neurons |
PMID: 32220623 |
Sample: Tissue homogenate |
DOI: 10.1111/jfbc.14306 |
|
PMID: 35766031 |
Sample: colon tissue,brain tissue |
DOI: 10.1111/jfbc.14397 |
|
PMID: 36069470 |
Sample: heart tissue |
PMID: 35453953 |
Sample: brain |
PMID: 36152772 |
Sample: brain tissue |
DOI: 10.1111/aji.13589 |
|
PMID: 35750632 |
Sample: serum |
Sample: plasma |
DOI: 10.21037/atm-22-483 |
|
PMID: 35280364 |
Sample: myocardium |
PMID: 33383446 |
Sample: lung tissue |
PMID: 33370579 |
Sample: SK-N-SH cell,IMR-32 cell |
Sample: serum |
Sample: kidney tissue |
PMID: 35389159 |
Sample: serum |
DOI: 10.21037/tau-22-505 |
|
Sample: kidney tissue |
Sample: serum |
DOI: 10.1155/2021/2073296 |
|
Sample: BEAS-2B cell |
Sample: hippocampal tissue |
PMID: 36252429 |
Sample: SK-N-SH cells |
DOI: 10.1111/jfbc.14129 |
|
PMID: 35298033 |
Sample: gastric,jejunal, colonic |
DOI: 10.1111/jfbc.13994 |
|
PMID: 34778972 |
Sample: brain |
DOI: 10.1111/fcp.12845 |
|
Sample: renal |
DOI: 10.3390/cimb44110358 |
|
PMID: 36354670 |
Sample: serum |
DOI: 10.3390/cimb45020057 |
|
Sample: colonic tissue |
Sample: serum,liver,skin |
PMID: 32796257 |
Sample: serum |
DOI: 10.1002/ddr.21581 |
|
Sample: liver |
Sample: brain |
Sample: plasma |
PMID: 32848317 |
Sample: Serum |
M*****aSubmitted [ Oct 14 2022 ]
Asked: Dear, does this kit have 10T specification?
Reply
adminSubmitted [ Oct 14 2022 ]
Answered: We cannot provide 10T size, and the specification minimum of biochemical kit is 48T.
M*******aSubmitted [ Oct 09 2022 ]
Asked: Can i use this to measure MDA in cell lysate?
Reply
adminSubmitted [ Oct 09 2022 ]
Answered: Hi Marchella, I'll reply you by mail. sincerely Amanda
M*****aSubmitted [ Sep 14 2022 ]
Asked: oxidative index in fish blood/serum: MDA?
Reply
adminSubmitted [ Sep 14 2022 ]
Answered: It can be detect the fish blood.