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Mouse CORT (Corticosterone) ELISA Kit

Citations(26)

Cat.No.:E-EL-0161
Reactivity: Mouse

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T/24
Assay time	2.0h
Detection range	3.12-200 ng/mL
Sensitivity	1.93 ng/mL
Sample type &Sample volume	serum and plasma; 50μL
Specificity	This kit recognizes CORT in samples. No significant cross-reactivity or interference between CORT and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of CORT concentrations in serum and plasma.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CORT. During the reaction, CORT in the sample or standard competes with a fixed amount of CORT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CORT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CORT in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 12 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level CORT were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level CORT were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	20.44	80.37	185.44	21.23	82.33	187.01
Standard deviation	1.99	6.70	14.13	2.10	7.31	15.00
CV (%)	9.73	8.34	7.62	9.88	8.88	8.02

Recovery

The recovery of CORT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	80-97	89
EDTA plasma(n=8)	81-93	85
Urine(n=8)	84-102	95

Linearity

Samples were spiked with high concentrations of Mouse CORT and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research Area

Signal transduction, Cancer, Epigenetics and Nuclear Signal, Neuroscience, Cardiovascular, Cell Biology, Stem cells, Metabolism

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

CHINESE JOURNAL OF CHEMISTRY (2021) IF: 6

Uncarialins J‐M from Uncaria rhynchophylla and their anti‐depression mechanism in unpredictable chronic mild stress‐induced mice via activating 5‐HT1A receptor

DOI: 10.1002/cjoc.202000652
	Sample: Lung

CHEMICO-BIOLOGICAL INTERACTIONS (2022) IF: 5.168

Binge alcohol consumption exacerbates high-fat diet-induced neurobehavioral anomalies: Possible underlying mechanisms

DOI: 10.1016/j.cbi.2022.110039
PMID: 35863473	Sample: Serum

MOLECULAR BIOLOGY REPORTS (2020) IF: 1.402

The role of hippocampal GABA A receptors on anxiolytic effects of Echium amoenum extract in a mice model of restraint stress

DOI: 10.1007/s11033-020-05699-7
PMID: 32778988	Sample: Serum

Current Medical Science (2021) IF: 2.135

Protective Effect of Hydroxysafflor Yellow A against Chronic Mild Stress-induced Memory Impairments by Suppressing Tau Phosphorylation in Mice

DOI: 10.1007/s11596-021-2369-3
PMID: 34129201	Sample: Serum

METABOLIC BRAIN DISEASE (2017) IF: 2.297

Combined corticosterone treatment and chronic restraint stress lead to depression associated with early cognitive deficits in mice

DOI: 10.1007/s11011-017-0148-4
	Sample: Plasma

Beneficial Microbes (2018) IF: 2.31

Anxiolytic-like effect of Bifidobacterium adolescentis IM38 in mice with or without immobilisation stress

DOI: 10.3920/BM2016.0226
	Sample: Serum,Cell Culture Supernatant

JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2018) IF: 2.478

High activity of the endogenous opioid system and acute but not chronic stress influence experimental colitis development in mice

DOI: 10.26402/jpp.2018.5.11
	Sample: Plasma

MOLECULAR REPRODUCTION AND DEVELOPMENT (2021) IF: 2.609

Dysfunction in Sertoli cells participates in glucocorticoid-induced impairment of spermatogenesis

DOI: 10.1002/mrd.23515
PMID: 34032349	Sample: Serum

PLoS One (2020) IF: 2.74

Hypergravity as a gravitational therapy mitigates the effects of knee osteoarthritis on the musculoskeletal system in a murine model

DOI: 10.1371/journal.pone.0243098
PMID: 33296408	Sample: Serum

BEHAVIOURAL BRAIN RESEARCH (2018) IF: 3.173

Antidepressant activity of vorinostat is associated with amelioration of oxidative stress and inflammation in a corticosterone-induced chronic stress model in mice

DOI: 10.1016/j.bbr.2018.02.009
PMID: 29452193	Sample: Serum

HORMONES AND BEHAVIOR (2017) IF: 3.378

What is stressful for females? Differential effects of unpredictable environmental or social stress in CD1 female mice

DOI: 10.1016/j.yhbeh.2017.11.013
	Sample: Plasma

NEUROCHEMISTRY INTERNATIONAL (2020) IF: 3.881

Hesperidin alleviates chronic restraint stress and lipopolysaccharide-induced Hippocampus and Frontal cortex damage in mice: Role of TLR4/NF-κB, p38 MAPK/JNK, Nrf2/ARE signaling

DOI: 10.1016/j.neuint.2020.104835
PMID: 32853749	Sample: Serum

Nutrients (2019) IF: 4.171

The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice

DOI: 10.3390/nu11040819
	Sample: Blood

PHYTOMEDICINE (2020) IF: 4.268

Uncaria rhynchophylla ameliorates unpredictable chronic mild stress-induced depression in mice via activating 5-HT1A receptor: Insights from transcriptomics

DOI: 10.1016/j.phymed.2020.153436
	Sample: Serum

Frontiers in Cellular and Infection Microbiology (2017) IF: 4.3

Microbiota Modulate Anxiety-Like Behavior and Endocrine Abnormalities in Hypothalamic-Pituitary-Adrenal Axis

DOI: 10.3389/fcimb.2017.00489
	Sample: Tissue Homogenate

Scientific Reports (2021) IF: 4.38

Buspirone alleviates anxiety, depression, and colitis; and modulates gut microbiota in mice

DOI: 10.1038/s41598-021-85681-w
PMID: 33731795	Sample: Plasma

BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545

Antidepressant and anti-amnesic effects of the aqueous lyophilisate of the leaves of Leptadenia arborea on an animal model of cognitive deficit associated depression

DOI: 10.1016/j.biopha.2020.110603
	Sample: Plasma

PSYCHONEUROENDOCRINOLOGY (2020) IF: 4.732

Differential modulation of GR signaling and HDACs in the development of resilient/vulnerable phenotype and antidepressant-like response of vorinostat

DOI: 10.1016/j.psyneuen.2020.105083
PMID: 33310695	Sample: Serum

JOURNAL OF PSYCHIATRIC RESEARCH (2021) IF: 4.791

Lipopolysaccharide exacerbates chronic restraint stress-induced neurobehavioral deficits: Mechanisms by redox imbalance, ASK1-related apoptosis, autophagic dysregulation

DOI: 10.1016/j.jpsychires.2021.10.021
PMID: 34768069	Sample: Serum

MOLECULAR NEUROBIOLOGY (2014) IF: 5.286

Olfactory Deprivation Hastens Alzheimer-Like Pathologies in a Human Tau-Overexpressed Mouse Model via Activation of cdk5

DOI: 10.1007/s12035-014-9007-z
	Sample: Plasma

Food & Function (2022) IF: 5.396

Perinatal Transmission of a Probiotic Bifidobacterium Strain Protects Against Early Life Stress-induced Mood and Gastrointestinal Motility Disorders

DOI: 10.1039/D2FO01164F
PMID: 35763064	Sample: Serum

Food & Function (2021) IF: 5.396

Pediococcus acidilactici CCFM6432 mitigates chronic stress-induced anxiety and gut microbial abnormalities

DOI: 10.1039/D1FO01608C
PMID: 34704999	Sample: Serum

Chinese Medicine (2021) IF: 5.455

Chaihu-Shugan-San (Shihosogansan) alleviates restraint stress-generated anxiety and depression in mice by regulating NF-κB-mediated BDNF expression through the modulation of gut microbiota

DOI: 10.1186/s13020-021-00492-5
PMID: 34391441	Sample: Blood

Oxidative Medicine and Cellular Longevity (2022) IF: 6.543

Small Noncoding RNAs Contribute to Sperm Oxidative Stress-Induced Programming of Behavioral and Metabolic Phenotypes in Offspring

DOI: 10.1155/2022/6877283
PMID: 35707281	Sample: Serum

Neurobiology of Stress (2022) IF: 7.142

The antidepressant effect of nucleus accumbens deep brain stimulation is mediated by parvalbumin-positive interneurons in the dorsal dentate gyrus

DOI: 10.1016/j.ynstr.2022.100492
	Sample: Serum

CELL (2023) IF: 66.85

Light modulates glucose metabolism by a retina-hypothalamus-brown adipose tissue axis

DOI: 10.1016/j.cell.2022.12.024
PMID: 36669474	Sample: Serum

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