Click here to view cell apoptosis assay products
×

Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit

  • Cat.No.:E-BC-K014

  • Detection instrument: Semi-automatic biochemical analyzer (546 nm), automatic biochemical analyzer (546 nm), Microplate re

To Purchase E-BC-K014

Size:
  • 96T
Price: $516
Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

 Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

NEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS). Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD). Then H2O2 react with TOOS and 4-amino-antipyrine (4-APP) to generate a colored substrate under the catalysis of peroxidase (POD). The colored substrate has a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and calculate the NEFA content indirectly.

Performance characteristics

Synonyms NEFA
Sample type Serum,plasma,tissue homogenate,cells,cell supernatant
Detection range 0.01-3.0 mmol/L
Detection method Colorimetric method
Assay type Quantitative
Other reagents required Normal saline (0.9% NaCl)
Storage 2-8℃
Valid period 12 months

Images

Liver free fatty acid was significantly increased by high-fat-high-cholesterol diet and DUSP22 knock out. (**P<0.01)

Chenxu Ge et al investigate the effect ROS that inhibits DUSP22 expression in fatty livers. Non-esterified Free Fatty Acid (NEFA) in mouse liver was determined using NEFA colorimetric assay kit (E-BC-K014).

Citations

  1. Nature Communications (2022) IF: 14.919
    The E3 ubiquitin-protein ligase Trim31 alleviates non-alcoholic fatty liver disease by targeting Rhbdf2 in mouse hepatocytes

    DOI: 10.1038/s41467-022-28641-w

    PMID: 35217669

    Sample: Serum
  2. Animals (2022) IF: 2.752
    Effect of TMR Briquettes on Milk Production, Nutrient Digestibility, and Manure Excretions of Dairy Cows in the Dry Zone of Sri Lanka

    DOI: 10.3390/ani12070932

    PMID: 35405920

    Sample: Serum
  3. BMC Endocrine Disorders (2022) IF: 3.263
    Low-dose spironolactone ameliorates adipose tissue inflammation and apoptosis in letrozole-induced PCOS rat model

    DOI: 10.1186/s12902-022-01143-y

    PMID: 36071485

    Sample: Adipose Tissue
  4. Nature Communications (2022) IF: 17.694
    Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK

    DOI: 10.1038/s41467-022-33493-5

    PMID: 36209205

    Sample: Serum
  5. IMMUNITY (2022) IF: 43.474
    Prolonged hypernutrition impairs TREM2-dependent efferocytosis to license chronic liver inflammation and NASH development

    DOI: 10.1016/j.immuni.2022.11.013

    PMID: 36521495

    Sample: Serum
Show AllShorten

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
Q

M*****iSubmitted [ Oct 21 2022 ]

Asked: My instrument does not have 546 nm, how should I detect it?

Reply

A

adminSubmitted [ Oct 21 2022 ]

Answered:  Choose the nearest wavelength if the instrument cannot be set to the wavelength required by this kit.

Q

M*****iSubmitted [ Sep 13 2022 ]

Asked: Is the sample of hemolytic serum available?

Reply

A

adminSubmitted [ Sep 13 2022 ]

Answered: Hemolytic sample will affect the result.

Q

S***********aSubmitted [ Jul 21 2022 ]

Asked: Can I request a quotation for this Cat#E-BC-K014

Reply

A

adminSubmitted [ Jul 21 2022 ]

Answered: The price of 96T E-BC-K014 is 516USD per kit.

... Show All Show Less

People Also Bought

Apply for FREE TRIAL Fluorometric Assay kit
*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?

*Captcha: