Introduction
PBS
buffer is a phosphate buffer, which provides a relatively stable ionic
environment and pH buffering capacity and is the most commonly used buffer for
biochemistry. When you prepare the WB samples, pre-cooled PBS are used to wash
the surface of tissues and internal debris or cell culture medium to prevent
the interference of endogenous IgG or culture medium.
Components
|
Cat
|
Products
|
100 mL
|
200 mL
|
500 mL
|
Storage
|
|
E-BC-R187
|
PBS Buffer, pH7.4 (10×)
|
100 mL
|
200 mL
|
500 mL
|
RT
|
Instructions
This product is a
10× concentrated solution, dilute with Deionized water to 1× working solution
before use.
For example: take
100 mL PBS buffer (10× ), dilute with Deionized water to 1 L.
1 × PBS buffer component
136.9 mM NaCl, 2.68 mM KCl, 10 mM
Na2HPO4•12H2O, 2 mM KH2PO4,
pH 7.4.
Storage
Store at room temperature for 12 months.
Cautions
1. PBS may precipitate when stored at low temperature. If PBS precipitates,
please dissolve PBS in water bath at 37°C, and then dilute it to 1 x working
buffer.
2. Keep the product sealed to prevent from pollution.
3. For
your safety and health, please wear the lab coat and disposable gloves before
the experiments.
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