Alternate Names	Interleukin-2 receptor subunit alpha,IL2RA,IL-2 receptor subunit alpha,IL-2-RA,IL-2R subunit alpha,IL2-RA,TAC antigen,p55,p55
Swissprot	P01589
References	1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Product Details

Form	Liquid
Clone No.	BC96
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	PE Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792D]
Reactivity	Human
Application	FCM
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE
	PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
FITC Anti-Human CD25 Antibody[BC96]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD25 Antibody[BC96]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Human CD25 Antibody[BC96]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human CD25 Antibody[BC96]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Human CD25 Antibody[BC96]	20Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Human CD25 Antibody[BC96]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Human CD25 Antibody[BC96]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Human CD25 Antibody[BC96]	20Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Human CD25 Antibody[BC96]	20Tests, 100Tests, 100Tests×2	Check More

Citations

MOLECULAR PHARMACEUTICS (2022) IF: 4.939

124I Radiolabeled Basiliximab for CD25-Targeted Immuno-PET Imaging of Activated T Cells

DOI: 10.1021/acs.molpharmaceut.2c00330
PMID: 35704773	Sample: Karpas299 Cell,Daudi Cell

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