Alternate Names	Neural cell adhesion molecule 1,NCAM1,N-CAM-1,NCAM-1,NCAM
Swissprot	P13591
References	1. Lanier L, et al. 1991. J. Immunol. 146:4421. 2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71. 3. Cremer H, et al. 1994. Nature 367:455.

Product Details

Form	Liquid
Clone No.	5.1H11
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	PE Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792D]
Reactivity	Human
Application	FCM
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE
	PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
FITC Anti-Human CD56/NCAM Antibody[5.1H11]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD56/NCAM Antibody[5.1H11]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Human CD56/NCAM Antibody[5.1H11]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Human CD56/NCAM Antibody[5.1H11]	20Tests, 100Tests, 100Tests×2	Check More

Citations

Nature Biomedical Engineering (2021) IF: 25.671

Restoration of dystrophin expression in mice by suppressing a nonsense mutation through the incorporation of unnatural amino acids

DOI: 10.1038/s41551-021-00774-1
PMID: 34341535	Sample: Myoblasts

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