Alternate Names	Interleukin-4,IL-4,B-cell IgG differentiation factor,B-cell growth factor 1,BSF-1,IGG1 induction factor
Swissprot	P07750
References	1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294. 3. Dullens H, et al. 1991. In vivo 5:567. 4. Paul W. 1991. Blood 77:1859.

Product Details

Form	Liquid
Clone No.	11B11
Host	Rat
Isotype	Rat IgG1, κ
Isotype Control	PE Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F09823D]
Reactivity	Mouse
Application	ICFCM
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE
	PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is a potent lymphoid cell growth factor which stimulates the growth and activation of certain B cells and T cells. IL-4 is important for regulation of T helper subset development.

Protocols & Troubleshootings

Related Protocols	Staining Intracellular Antigens for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse IL-4 Antibody[11B11]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse IL-4 Antibody[11B11]	25μg, 100μg	Check More
PE Anti-Mouse IL-4 Antibody[11B11]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse IL-4 Antibody[11B11]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse IL-4 Antibody[11B11]	25μg, 100μg	Check More

Citations

Frontiers in Immunology (2022) IF: 7.561

Targeting Glutamine Metabolism Ameliorates Autoimmune Hepatitis via Inhibiting T Cell Activation and Differentiation.

DOI: 10.3389/fimmu.2022.880262
	Sample: Splenocytes

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