Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PE/Cyanine5.5 Anti-Mouse CD49b/pan-NK cells Antibody[DX5]
Cat.No.:E-AB-F1116UI
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Alternate Names | Integrin alpha-2,CD49 antigen-like family member B,Collagen receptor,Platelet membrane glycoprotein Ia,GPIa,VLA-2 subunit alpha,CD49b |
Swissprot | Q62469 |
References |
1. Arase H, et al. 2001. J. Immunol. 167:1141.
2. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 3. Sasaki K, et al. 2003. Int. Immunol. 15:701. 4. Inoue O, et al. 2003. J. Cell Biol. 160:769. |
Form | Liquid |
Clone No. | DX5 |
Host | Rat |
Isotype | Rat IgM, κ |
Isotype Control | [Product E-AB-F09773I] |
Reactivity | Mouse |
Application | FCM |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE/Cyanine5.5 |
PE/Cyanine5.5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 690 nm (e.g., a 690/50 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
DX5 antigen has been recently characterized as CD49b. It is a 150 kD integrin α chain also known as α2 integrin, VLA-2 α chain, and integrin α2 chain. CD49b non-covalently associates with CD29 (β1 integrin) to form the CD49b/CD29 complex known as VLA-2, a receptor for collagen and laminin. CD49b is expressed on platelets, the majority of NK cells, NKT cells, and a small subset of CD8+ T cells (this population can be significantly increased following viral infection). DX5 is used for the identification and isolation of NK cells, and is especially useful for identifying NK cells in mice lacking the NK1.1 antigen. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |