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Cat.No.:E-AB-F1352P
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Alternate Names | T-cell surface glycoprotein CD4,T-cell surface antigen T4/Leu-3 |
Swissprot | P01730 |
Gene ID | 920 |
References |
1. Center D et al. 1996. Immunol. Today 17:476.
2. Gaubin M et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723. |
Form | Liquid |
Clone No. | SK3 |
Host | Mouse |
Isotype | Mouse IgG1, κ |
Isotype Control | PE/Elab Fluor® 594 Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792P] |
Reactivity | Human |
Application | FCM |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE/Elab Fluor® 594 |
PE/Elab Fluor® 594 is designed to be excited by the blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 620 nm (e.g., a 610/20 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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