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Potassium (K) Turbidimetric Assay Kit

  • Cat.No.:E-BC-K279-M

  • Detection instrument: Microplate reader(450 nm-600 nm,optimum wavelength: 450 nm)

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  • 48T
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Detection principle

Under the alkaline condition, the sodium tetraphenylborate reacts with the potassium ions in the sample to form the potassium tetraphenylborate which is white and small particles with small solubility. Potassium tetraphenylborate particles are in a stable suspension state in the solution. The turbidity is proportional to the potassium ion concentration in the sample and potassium content can be calculated indirectly by measuring the OD value at 450 nm.

Performance characteristics

Synonyms K
Sample type Serum,plasma,milk,animal tissue,cells,cell culture supernatant
Sensitivity 0.002 mmol/L
Detection range 0.01-0.80 mmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 20 min
Precision Average inter-assay CV: 6.100%Average intra-assay CV: 1.100%
Other instruments required Centrifuge, Micropipettor, Vortex mixer
Storage 2-8℃
Valid period 12 months

Images

Potassium concentration was significantly changed after treating with DSS and other substances. (*P<0.05, **P<0.01, ***P<0.001)

B Zeng et al investigate the mechanism of dextran sodium sulfate potentiating NLRP3 inflammasome activation. Potassium (K) level in cell supernatant was determined using potassium turbidimetic assay kit (E-BC-K279-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the formal experiment and the detection range (0.01-0.80 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Rat serum

1

RAW 264.7 cellular supernatant

1

Human plasma

1

Human milk

1

10% Rat liver tissue homogenization

2-4

Note: The diluent is deionized water.

Citations

  1. Frontiers in Nutrition (2021) IF: 6.576
    Microbial-Assisted Wheat Iron Biofortification Using Endophytic Bacillus altitudinis WR10.

    DOI: 10.3389/fnut.2021.704030

    Sample: Wheat Tissue
  2. ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2020) IF: 2.575
    Effect of Acacia senegal on TGF-β1 and vascular mediators in a rat model of diabetic nephropathy

    DOI: 10.1080/13813455.2020.1781901

    PMID: 32574082

    Sample: Serum
  3. INTERNATIONAL JOURNAL OF BIOMETEOROLOGY (2022) IF: 3.787
    Physiological, blood-biochemical and behavioural changes of Ghoongroo pigs in seasonal heat stress of a hot-humid tropical environment

    DOI: 10.1007/s00484-022-02281-7

    PMID: 35384502

    Sample: Serum
  4. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2021) IF: 4.219
    Coconut products alleviate hyperglycaemic, hyperlipidimic and nephropathy indices in streptozotocin-induced diabetic wistar rats

    DOI: 10.1016/j.sjbs.2021.06.060

    PMID: 34354403

    Sample: Serum
  5. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2023) IF: 4.925
    Targeting Noncanonical Pyroptosis With a Small Molecular Inhibitor Alleviates Inflammation in the LPS-Induced Keratitis Mouse Model

    DOI: 10.1167/iovs.64.1.1

    Sample: Thp-1 Cell
  6. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
    Dimethyl fumarate ameliorates autoimmune hepatitis in mice by blocking NLRP3 inflammasome activation

    DOI: 10.1016/j.intimp.2022.108867

    PMID: 35605433

    Sample: Bmdm
  7. PLANT PHYSIOLOGY AND BIOCHEMISTRY (2023) IF: 5.437
    Dioscorea composita WRKY12 is involved in the regulation of salt tolerance by directly activating the promoter of AtRCI2A

    DOI: 10.1016/j.plaphy.2023.02.020

    Sample: Seed
  8. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Ameliorative Effects of a Rhenium (V) Compound with Uracil-Derived Ligand Markers Associated with Hyperglycaemia-Induced Renal Dysfunction in Diet-Induced Prediabetic Rats

    DOI: 10.3390/ijms232315400

    Sample: Plasma,Urine
  9. Frontiers in Plant Science (2022) IF: 6.627
    Halotolerant Bacillus altitudinis WR10 improves salt tolerance in wheat via a multi-level mechanism

    DOI: 10.3389/fpls.2022.941388

    PMID: 35909740

    Sample: Roots Or Shoots
  10. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2022) IF: 7.088
    Discovery of a novel and potent inhibitor with differential species-specific effects against NLRP3 and AIM2 inflammasome-dependent pyroptosis

    DOI: 10.1016/j.ejmech.2022.114194

    PMID: 35183871

    Sample: Macrophages
  11. Frontiers in Immunology (2021) IF: 7.561
    NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection

    DOI: 10.3389/fimmu.2021.747370

    Sample: J774A.1 Cell
  12. Cellular & Molecular Immunology (2022) IF: 22.096
    Dextran sodium sulfate potentiates NLRP3 inflammasome activation by modulating the KCa3.1 potassium channel in a mouse model of colitis

    DOI: 10.1038/s41423-022-00891-0

    PMID: 35799057

    Sample: Bmdms
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Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
Q

M*****iSubmitted [ Aug 01 2022 ]

Asked: Can I use the biochemical analyzer for the detection?

Reply

A

adminSubmitted [ Aug 01 2022 ]

Answered: Unfortunately, this kit is only suitable for microplate reader.

Q

M*****iSubmitted [ Aug 01 2022 ]

Asked: Is this kit suitable for plant leaves?

Reply

A

adminSubmitted [ Aug 01 2022 ]

Answered: Yes,this kit can detect plant leaves.

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