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Potassium (K) Turbidimetric Assay Kit

Citations(12)

Cat.No.:E-BC-K279-M
Detection instrument: Microplate reader(450 nm-600 nm,optimum wavelength: 450 nm)

Detection method: Colorimetric method

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Detection principle

Under the alkaline condition, the sodium tetraphenylborate reacts with the potassium ions in the sample to form the potassium tetraphenylborate which is white and small particles with small solubility. Potassium tetraphenylborate particles are in a stable suspension state in the solution. The turbidity is proportional to the potassium ion concentration in the sample and potassium content can be calculated indirectly by measuring the OD value at 450 nm.

Performance characteristics

Synonyms	K
Sample type	Serum,plasma,milk,animal tissue,cells,cell culture supernatant
Sensitivity	0.002 mmol/L
Detection range	0.01-0.80 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 6.100%Average intra-assay CV: 1.100%
Other instruments required	Centrifuge, Micropipettor, Vortex mixer
Storage	2-8℃
Valid period	12 months

Images

Potassium concentration was significantly changed after treating with DSS and other substances. (*P<0.05, **P<0.01, ***P<0.001)

B Zeng et al investigate the mechanism of dextran sodium sulfate potentiating NLRP3 inflammasome activation. Potassium (K) level in cell supernatant was determined using potassium turbidimetic assay kit (E-BC-K279-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the formal experiment and the detection range (0.01-0.80 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Rat serum	1
RAW 264.7 cellular supernatant	1
Human plasma	1
Human milk	1
10% Rat liver tissue homogenization	2-4

Note: The diluent is deionized water.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Frontiers in Nutrition (2021) IF: 6.576

Microbial-Assisted Wheat Iron Biofortification Using Endophytic Bacillus altitudinis WR10.

DOI: 10.3389/fnut.2021.704030
	Sample: Wheat Tissue

ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2020) IF: 2.575

Effect of Acacia senegal on TGF-β1 and vascular mediators in a rat model of diabetic nephropathy

DOI: 10.1080/13813455.2020.1781901
PMID: 32574082	Sample: Serum

INTERNATIONAL JOURNAL OF BIOMETEOROLOGY (2022) IF: 3.787

Physiological, blood-biochemical and behavioural changes of Ghoongroo pigs in seasonal heat stress of a hot-humid tropical environment

DOI: 10.1007/s00484-022-02281-7
PMID: 35384502	Sample: Serum

SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2021) IF: 4.219

Coconut products alleviate hyperglycaemic, hyperlipidimic and nephropathy indices in streptozotocin-induced diabetic wistar rats

DOI: 10.1016/j.sjbs.2021.06.060
PMID: 34354403	Sample: Serum

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2023) IF: 4.925

Targeting Noncanonical Pyroptosis With a Small Molecular Inhibitor Alleviates Inflammation in the LPS-Induced Keratitis Mouse Model

DOI: 10.1167/iovs.64.1.1
	Sample: Thp-1 Cell

INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932

Dimethyl fumarate ameliorates autoimmune hepatitis in mice by blocking NLRP3 inflammasome activation

DOI: 10.1016/j.intimp.2022.108867
PMID: 35605433	Sample: Bmdm

PLANT PHYSIOLOGY AND BIOCHEMISTRY (2023) IF: 5.437

Dioscorea composita WRKY12 is involved in the regulation of salt tolerance by directly activating the promoter of AtRCI2A

DOI: 10.1016/j.plaphy.2023.02.020
	Sample: Seed

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208

Ameliorative Effects of a Rhenium (V) Compound with Uracil-Derived Ligand Markers Associated with Hyperglycaemia-Induced Renal Dysfunction in Diet-Induced Prediabetic Rats

DOI: 10.3390/ijms232315400
	Sample: Plasma,Urine

Frontiers in Plant Science (2022) IF: 6.627

Halotolerant Bacillus altitudinis WR10 improves salt tolerance in wheat via a multi-level mechanism

DOI: 10.3389/fpls.2022.941388
PMID: 35909740	Sample: Roots Or Shoots

EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2022) IF: 7.088

Discovery of a novel and potent inhibitor with differential species-specific effects against NLRP3 and AIM2 inflammasome-dependent pyroptosis

DOI: 10.1016/j.ejmech.2022.114194
PMID: 35183871	Sample: Macrophages

Frontiers in Immunology (2021) IF: 7.561

NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection

DOI: 10.3389/fimmu.2021.747370
	Sample: J774A.1 Cell

Cellular & Molecular Immunology (2022) IF: 22.096

Dextran sodium sulfate potentiates NLRP3 inflammasome activation by modulating the KCa3.1 potassium channel in a mouse model of colitis

DOI: 10.1038/s41423-022-00891-0
PMID: 35799057	Sample: Bmdms

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Reviews/Q&A

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Reviews
Q&A

M*****iSubmitted [ Aug 01 2022 ]

Asked: Can I use the biochemical analyzer for the detection?

adminSubmitted [ Aug 01 2022 ]

Answered: Unfortunately, this kit is only suitable for microplate reader.

M*****iSubmitted [ Aug 01 2022 ]

Asked: Is this kit suitable for plant leaves？

adminSubmitted [ Aug 01 2022 ]

Answered: Yes,this kit can detect plant leaves.

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