Introduction
Compared with nitrocellulose
membranes, hydrophobic polyvinylidene fluoride (PVDF) membranes have various
advantages, for example, good mechanical strength, strong protein retention,
low background, extensive solvent compatibility, and excellent coloring ability.
The PVDF membrane with a pore size of 0.22 μm can be used for most immunoblots
and is recommended for proteins with a molecular weight less than 20 kDa.
Components
|
Cat
|
Products
|
Size
|
Storage
|
|
E-BC-R329
|
PVDF Membrane (0.22 μm, 8.5 cm × 6 cm)
|
10 pieces
|
20 pieces
|
RT
|
|
PVDF Membrane (0.22 μm, 13.25 cm × 15 cm)
|
10 pieces
|
20 pieces
|
Binding ability: Insulin: 262 μg/cm2
BSA: 340 μg/cm2
Goat IgG: 448 μg/cm2
Instructions
1. Soak the PVDF membrane in methanol for 1 min
to activate it.
2. Soak
the activated PVDF membrane with deionized water to remove the methanol from
the membrane.
3. Immerse
the PVDF membrane in the transfer buffer for 2~3 min, and the blotting filter
paper too.
4. Place
the following material in the order of the black plate (negative electrode) -
fiber mat - filter paper - gel - PVDF membrane - filter paper - fiber mat -
white plate (positive electrode) in order,
discharge
bubbles, clamp and place in the
wet transfer tank.
5. Follow the Western Blot steps.
Storage
Store at room temperature for 12 months.
Cautions
1. Please
wear gloves to prevent contamination of the PVDF membrane.
2. Do
not dry the PVDF membrane during the experiment.
3. If
you need other sizes of PVDF membrane and filter paper, please let us know in
advance, we will be happy to help you.
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