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Pyruvic Acid Colorimetric Assay Kit

  • Cat.No.:E-BC-K130-M

  • Detection instrument: Microplate reader(480-520 nm,optimum wavelength: 505 nm)

To Purchase E-BC-K130-M

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  • 96T
  • 48T
Price: $220
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Protocols for metabolism assays

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Detection principle

Pyruvic acid can react with chromogenic agent and the reaction product is reddish brown in alkaline solution. The depth of color is directly proportional to the pyruvate content. The pyruvate content can be calculated by measuring the OD value at 505 nm.

Performance characteristics

Sample type Serum,plasma,tissue
Sensitivity 0.003 μmol/mL
Detection range 0.003-2.0 μmol/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 30 min
Precision Average inter-assay CV: 3.600%Average intra-assay CV: 2.300%
Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Images

The concentration of pyruvic acid was increased significantly after treated with nanoparticles.

S J Tseng et al developed a nanoparticle for non-small cell lung cancer virotherapy. Pyruvic acid levels in mouse tumor cell culture medium was determined using pyruvic acid colorimetric assay kit (E-BC-K130-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.003-2.0 μmol/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Mouse serum

1

Mouse plasma

1

10% Mouse liver tissue homogenate

1

10% Rat kidney tissue homogenate

1

10% Rat heart tissue homogenate

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

  1. Frontiers in Molecular Biosciences (2022) IF: 6.113
    Glucose Deprivation Induced by Acarbose and Oncolytic Newcastle Disease Virus Promote Metabolic Oxidative Stress and Cell Death in a Breast Cancer Model

    DOI: 10.3389/fmolb.2022.816510

    PMID: 35936786

    Sample: Mammary Adenocarcinoma Tissue
  2. BIOTECHNOLOGY AND BIOENGINEERING (2022) IF: 4.395
    Physiological metabolic topology analysis of Halomonas elongata DSM 2581T in response to sodium chloride stress

    DOI: 10.1002/bit.28222

    PMID: 36062959

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