QuicKey Human ALB(Albumin) ELISA Kit

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This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ALB. Samples (or Standards) and biotinylated detection antibody specific for Human ALB are added to the micro ELISA plate wells. Human ALB would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ALB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human ALB. You can calculate the concentration of Human ALB in the samples by comparing the OD of the samples to the standard curve.

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ALB were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ALB were tested on 3 different plates, 20 replicates in each plate.

The recovery of Human ALB spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

ALB is the most abundant protein in human plasma and serum. This protein is normally present in serum at concentrations ranging from 30 to 50 mg/mL and accounts for approximately 60% of the total protein content in serum [1]. ALB has a molecular weight of 66.7 kDa and is composed of a single polypeptide chain with 585 amino acids and 17 disulfide bonds. ALB is involved in many physiological processes. For instance, this protein aids in the regulation of osmotic pressure and pH in blood. In addition, ALB mediates lipid metabolism, sequesters toxins, and works as an antioxidant (i.e., it binds free radicals) [2]. Another important function of ALB is its action as a transport protein for a wide range of solutes that include some low mass hormones, fatty acids, and drugs

1. Hassan M, Azzazy E, Christenson R H. All About Albumin: Biochemistry, Genetics, and Medical Applications. Theodore Peters, Jr. San Diego, CA: Academic Press, 1996, 432 pp, $85.00. ISBN 0-12-552110-3[J]. 1997.

2. Otagiri M. A molecular functional study on the interactions of drugs with plasma proteins [J]. Drug metabolism and pharmacokinetics, 2005, 20(5): 309-323.