QuicKey Human BMG/β2-MG(Beta-2-Microglobulin) ELISA Kit

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This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BMG/β2-MG. Samples (or Standards) and biotinylated detection antibody specific for Human BMG/β2-MG are added to the micro ELISA plate wells. Human BMG/β2-MG would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMG/β2-MG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human BMG/β2-MG. You can calculate the concentration of Human BMG/β2-MG in the samples by comparing the OD of the samples to the standard curve.

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human BMG/β2-MG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human BMG/β2-MG were tested on 3 different plates, 20 replicates in each plate.

The recovery of Human BMG/β2-MG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Beta 2 microglobulin (β2M) is a small protein (11,800 Dalton), normally found on the surface of many cells, including lymphocytes, and in small amounts in the blood and urine [1]. An increased amount in the blood or urine may be a sign of certain diseases, including some types of cancer, such as multiple myeloma or lymphoma.β2M is critical for the immune surveillance and modulation in vertebrate animals. The dysregulation of β2M is associated with multiple diseases, including endogenous and infectious diseases. β2M could directly participate in the development of cancer cells, and the level of β2M is deemed as a prognostic marker for several malignancies. It also involves in forming major histocompatibility complex (MHC class I or MHC I) or like heterodimers, covering from antigen presentation to immune homeostasis [2].

1. Güssow D, Rein R, Ginjaar I, et al. The human beta 2-microglobulin gene. Primary structure and definition of the transcriptional unit. [J]. Journal of Immunology, 1987, 139(9):3132-8.

2. Ohta Y, Shiina T, Lohr R L, et al. Primordial linkage of 2-microglobulin to the MHC [J]. Journal of Immunology, 2011, 186(6):3563-3571.