QuicKey Human CRP(C-Reactive Protein) ELISA Kit

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This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRP. Samples (or Standards) and biotinylated detection antibody specific for Human CRP are added to the micro ELISA plate wells. Human CRP would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human CRP. You can calculate the concentration of Human CRP in the samples by comparing the OD of the samples to the standard curve.

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CRP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CRP were tested on 3 different plates, 20 replicates in each plate.

The recovery of Human CRP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

C-reactive protein (CRP) is a member of the phylogenetically ancient and highly conserved ‘pentraxin’ family of proteins, which also includes serum amyloid P component (SAP), a constituent of all amyloid deposits [1]. In man and other animal species, CRP is a major acute-phase plasma protein displaying rapid and pronounced rise of its serum concentration in response to infection or tissue injury.CRP is used mainly as a marker of inflammation. Apart from liver failure, there are few known factors that interfere with CRP production. Interferon alpha inhibits CRP production from liver cells which may explain the relatively low levels of CRP found during viral infections compared to bacterial infections [2]. Measuring and charting CRP values can prove useful in determining disease progress or the effectiveness of treatments. ELISA, immunoturbidimetry, nephelometry, rapid immunodiffusion, and visual agglutination are all methods used to measure CRP.

1. Gewurz, Henry. Biology of C-Reactive Protein and the Acute Phase Response [J]. Hospital Practice, 1982, 17(6):67-81.

2. Enocsson H , Christopher Sjwall, Skogh T , et al. Interferon-alpha mediates suppression of C-reactive protein: explanation for muted C-reactive protein response in lupus flares? [J]. Arthritis & Rheumatism, 2010, 60(12):3755-3760.