QuicKey Human IgE(Immunoglobulin E) ELISA Kit

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This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgE. Samples (or Standards) and biotinylated detection antibody specific for Human IgE are added to the micro ELISA plate wells. Human IgE would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human IgE. You can calculate the concentration of Human IgE in the samples by comparing the OD of the samples to the standard curve.

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IgE were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IgE were tested on 3 different plates, 20 replicates in each plate.

The recovery of Human IgE spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Immunoglobulin E (IgE), named in 1968 [1], was the last of the five classes of human antibodies to be discovered, and today is commonly associated with the various manifestations of allergic disease. IgE’s receptor-binding activities present unique features. There are two principal receptors, FcεRⅠ, structurally homologous to other members of the FcγR family, and FcεRⅡ/CD23. Adult serum IgE levels range from 20 to 200IU/mL and are generally considered to be abnormally elevated when they are greater than 333IU/mL (800ng/mL). Common diseases associated with IgE elevation include allergic asthma, seasonal allergic rhinitis, atopic dermatitis, drug-induced interstitial pneumonia, bronchopulmonary aspergillosis, leprosy, pemphigoid and certain parasitic infections [2].

1. Platts-Mills T A E, Heymann P W, Commins S P, et al. The discovery of IgE 50 years later[J]. Annals of Allergy, Asthma & Immunology, 2016, 116(3): 179-182.

2. Campbell A M, Vachier I, Chanez P, et al. Expression of the high-affinity receptor for IgE on bronchial epithelial cells of asthmatics[J]. American journal of respiratory cell and molecular biology, 1998, 19(1): 92-97.