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QuicKey Human NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit

  • Cat.No.:E-TSEL-H0003

  • Reactivity: Human

To Purchase E-TSEL-H0003

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $390
Qty:

Product Details

QuicKey Series

Get more sensitive and precise results with saving at least 1h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 2.5h
Detection range 0.13-8 ng/mL
Sensitivity 0.06 ng/mL
Sample type &Sample volume serum, plasma, urine; 50μL
Specificity This kit recognizes Human NGAL in samples. No significant or interference between Human NGAL and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human NGAL concentrations in serum, plasma, urine.Please consult technical support for the applicability if other biological fluids need to be tested.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NGAL. Samples (or Standards) and biotinylated detection antibody specific for Human NGAL are added to the micro ELISA plate wells. Human NGAL would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NGAL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human NGAL. You can calculate the concentration of Human NGAL in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.
ItemSpecificationsStorage conditions after test
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
2-8℃, 1 month
Reference Standard 96T: 2 vials
48T: 1 vial
Discard unused reconstituted standard and dilutions
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8℃
Biotinylated Detection Ab Working Solution 1 vial, 6 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
2-8℃ (Protect from light)
Substrate Reagent 1 vial, 10 mL
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Technical Data

Sample Values

sandwich-Ab-ELISA-Elabscience

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human NGAL were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human NGAL were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.37 0.90 3.57 0.35 0.81 3.73
Standard deviation 0.02 0.04 0.16 0.02 0.04 0.17
CV (%) 5.41 4.44 4.48 5.71 4.94 4.56

Recovery

The recovery of Human NGAL spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-100 94
EDTA plasma(n=8) 101-110 104
Urine(n=8) 87-98 90

Linearity

Samples were spiked with high concentrations of Human NGAL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

sandwich-Ab-ELISA-Elabscience

Target Information

Background

Neutrophil gelatinase associated lipocalin (NGAL) also known as Lipocalin 2 or Lcn2 is a 25 kDa, is a member of the lipocalin superfamily and a pleiotropic mediator of various inflammatory processes [1].NGAL, identified originally as a protein associated with matrix metalloproteinase 9 (MMP-9) of human neutrophils. Lipocalins are extracellular proteins which share a common tertiary structure that forms a barrel-like hydrophobic ligand binding site. When bound to MMP-9, NGAL protects it from proteolytic degradation sustaining the proteolytic activity of MMP-9. Neutrophil gelatinase-associated lipocalin (NGAL), is released by various cell types and is an attractive biomarker of inflammation, ischemia, infection, and kidney damage. Both intestinal and metabolic inflammation, as observed in obesity and related disorders, are associated with increased NGAL synthesis [2]. While NGAL in the intestinal tract regulates the composition of the gut microbiota and shows anti-inflammatory activities, it also exhibits proinflammatory activities in other experimental settings. Plasma NGAL is a novel marker of kidney function, which correlates to duration of end-stage renal failure (ESRD) and serum creatinine in uremic patients awaiting kidney transplantation. Plasma NGAL is associated with homocysteine in transplanted patients.
1. Kjeldsen L, Cowland J B, Borregaard N. Human neutrophil gelatinase-associated lipocalin and homologous proteins in rat and mouse.[J]. Biochim Biophys Acta, 2000, 1482(1):272-283.
2. Tilg, H., & Moschen, A. R. Adipocytokines: mediators linking adipose tissue, inflammation and immunity. Nature Reviews Immunology, 2006, 6(10), 772.
Database LinksSwissProt: P80188
Entrez Gene: 3934
Research AreaCancer, Cardiovascular, Metabolism, Immunology, Signal transduction

Assay Procedures

step01

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C

step02

2. Aspirate and wash the plate for 3 times

step03

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

step04

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

step05

5. Add 50μL Stop Solution

step06

6. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021) IF: 5.31
    Identification of key candidate biomarkers for severe influenza infection by integrated bioinformatical analysis and initial clinical validation

    DOI: 10.1111/jcmm.16275

    PMID: 33448094

    Sample: Plasma
  2. Scientific Reports (2021) IF: 4.38
    Plasma lipocalin-2/NGAL is stable over 12 weeks and is not modulated by exercise or dieting

    DOI: 10.1038/s41598-021-83472-x

    Sample: Plasma
  3. JOURNAL OF PERIODONTAL RESEARCH (2022) IF: 4.419
    Gingival crevicular fluid lipocalin-2 and semaphorin3A in stage III periodontitis: Non-surgical periodontal treatment effects

    DOI: 10.1111/jre.12995

    PMID: 35468224

    Sample: gingival crevicular fluid (GCF)
  4. Toxins (2021) IF: 4.546
    Biomarkers of Uremic Cardiotoxicity

    DOI: 10.3390/toxins13090639

    PMID: 34564643

    Sample: serum
  5. CANCER CHEMOTHERAPY AND PHARMACOLOGY (2019) IF: 3.008
    Serum neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for predicting high dose methotrexate associated acute kidney injury in children with acute lymphoblastic leukemia

    DOI: 10.1007/s00280-019-03980-6

    PMID: 31676986

    Sample: Serum
  6. RENAL FAILURE (2022) IF: 3.222
    Urinary NGAL, IGFBP-7, and TIMP-2: novel biomarkers to predict contrast medium-induced acute kidney injury in children

    DOI: 10.1080/0886022X.2022.2075277

    PMID: 36120960

    Sample: Serial urinary samples
  7. PLoS One (2021) IF: 3.24
    Effects of pneumoperitoneum on kidney injury biomarkers: A randomized clinical trial

    DOI: 10.1371/journal.pone.0247088

    PMID: 33606739

    Sample: Plasma
  8. CURRENT PHARMACEUTICAL DESIGN (2023) IF: 3.31
    Renal Stem Cells, Renal Resistive Index, and Neutrophil Gelatinase Associated Lipocalin Changes After Revascularization in Patients With Renovascular Hypertension and Ischemic Nephropathy

    DOI: 10.2174/1381612829666221213104945

    Sample: serum
  9. PeerJ (2019) IF: 2.353
    The role of serum and urinary biomarkers in the diagnosis of early diabetic nephropathy in patients with type 2 diabetes

    DOI: 10.7717/peerj.7079

    Sample: urinary,serum
  10. BMC Nephrology (2022) IF: 2.388
    Association of plasma neutrophil gelatinase-associated lipocalin and thoracic aorta calcification in maintenance hemodialysis patients with and without diabetes

    DOI: 10.1186/s12882-022-02773-z

    PMID: 35459121

    Sample: plasma
  11. CHRONOBIOLOGY INTERNATIONAL (2020) IF: 2.486
    Assessment of acute aerobic exercise in the morning versus evening on asprosin, spexin, lipocalin-2, and insulin level in overweight/obese versus normal weight adult men

    DOI: 10.1080/07420528.2020.1792482

    PMID: 32741294

    Sample: Serum
  12. JOURNAL OF BONE AND MINERAL METABOLISM (2021) IF: 2.626
    Association of plasma neutrophil gelatinase-associated lipocalin with parameters of CKD–MBD in maintenance hemodialysis patients

    DOI: 10.1007/s00774-021-01248-9

    Sample: plasma
  13. SAUDI MEDICAL JOURNAL (2020) IF: 1.195
    Evaluation of early biomarkers of renal dysfunction in diabetic patients.

    DOI: 10.15537/smj.2020.7.25168

    Sample: Urinary
  14. Journal of Maternal-Fetal & Neonatal Medicine (2019) IF: 1.569
    Perinatal lipocalin-2 profile at the extremes of fetal growth

    DOI: 10.1080/14767058.2019.1659774

    Sample: milk,serum
  15. Indian Heart Journal (2022)
    Prognostic utility of neutrophil gelatinase associated lipocalin in cardiac ICU: A prospective study

    DOI: 10.1016/j.ihj.2022.03.004

    Sample: serum,urinary
  16. Brazilian Journal of Anesthesiology (2022)
    Impact of colloids or crystalloids in renal function assessed by NGAL and KIM-1 after hysterectomy: randomized controlled trial

    DOI: 10.1016/j.bjane.2021.10.009

    PMID: 34848313

    Sample: Urine
  17. Physiological Reports (2017)
    Impact of acute versus repetitive moderate intensity endurance exercise on kidney injury markers

    DOI: 10.14814/phy2.13544

    Sample: urine
  18. Physiological Reports (2018)
    Impact of acute versus prolonged exercise and dehydration on kidney function and injury

    DOI: 10.14814/phy2.13734

    Sample: Urine
  19. Diabetes & Metabolic Syndrome-Clinical Research & Reviews (2018)
    Assessing urinary levels of IL-18, NGAL and albumin creatinine ratio in patients with diabetic nephropathy

    DOI: 10.1016/j.dsx.2018.11.022

    PMID: 30641767

    Sample: Urine
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