QuicKey Human PCT(Procalcitonin) ELISA Kit

Get more sensitive and precise results with saving at least 1h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PCT. Samples (or Standards) and biotinylated detection antibody specific for Human PCT are added to the micro ELISA plate wells. Human PCT would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human PCT. You can calculate the concentration of Human PCT in the samples by comparing the OD of the samples to the standard curve.

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PCT were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PCT were tested on 3 different plates, 20 replicates in each plate.

The recovery of Human PCT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Procalcitonin (PCT), the precursor of the hormone calcitonin, comprises 116 amino acids and is present in minute quantities under healthy conditions[1]. PCT and other calcitonin precursors in serum are present at less than 50 pg/mL in healthy individuals, but are highly elevated in serum where conditions leading to systemic inflammatory response syndrome or sepsis prevail. The level of PCT has been observed to increase significantly with bacterial infection and correlate to the severity of the infection. Therefore, serum PCT has been considered a powerful biomarker for the diagnosis of bacterial infection[2]. PCT has been proven to have high accuracy as a marker of infection in adult patients, particularly in sepsis. In children it has been investigated in several conditions, including neonatal sepsis, severe infection in neutropenic children, and other specific bacterial infections (e.g. urinary tract infections, pneumonia, bone and joint tissue infections).

1. Christ-Carin M. Procalcitonin in bacterial infections-hype, hope, more or less?[J]. Swiss medical weekly, 2005, 135(3132).

2. Brechot N, Hekimian G, Chastre J, et al. Procalcitonin to guide antibiotic therapy in the ICU[J]. International journal of antimicrobial agents, 2015, 46: S19-S24.