QuicKey Human TG(Thyroglobulin) ELISA Kit

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This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TG. Samples (or Standards) and biotinylated detection antibody specific for Human TG are added to the micro ELISA plate wells. Human TG would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human TG. You can calculate the concentration of Human TG in the samples by comparing the OD of the samples to the standard curve.

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TG were tested on 3 different plates, 20 replicates in each plate.

The recovery of Human TG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Thyroglobulin (Tg) is a large glycoprotein that is stored in follicles in healthy thyroid tissue as colloid where it acts as a substrate for thyroid hormones synthesis. Thyroglobulin is a homologous dimer with a molecular weight of about 660 kDa. Human protein consists of 2,767 amino acid residues and is the storage form of iodine in the thyroid gland, precursor of the iodinated thyroid hormones, thyroxine (T4) and triiodothyronine (T3). It is exclusively produced by normal or well-differentiated malignant thyrocytes. Then, its tissue-specific origin makes it eminently suitable as a serum tumor indicator in patients followed-up for differentiated thyroid carcinoma (DTC) after thyroid removal and remnants ablation[1].The most studied polymorphisms in the TG gene are rs2069550 (E10SNP158), rs853326 (E12SNP), and rs180223 (E10SNP24) in exons 10–12 and rs2076740 (E33SNP) in exon 33 SNP, which were reported to be associated with AITD[2].

1. Grebe S K G. Diagnosis and management of thyroid carcinoma: a focus on serum thyroglobulin[J]. Expert Review of Endocrinology & Metabolism, 2009, 4(1): 25-43.

2. Wang L Q, Wang T Y, Sun Q L, et al. Correlation between thyroglobulin gene polymorphisms and autoimmune thyroid disease[J]. Molecular medicine reports, 2015, 12(3): 4469-4475.