QuicKey Human VEGF-A(Vascular Endothelial Growth Factor A) ELISA Kit

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This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGF-A. Samples (or Standards) and biotinylated detection antibody specific for Human VEGF-A are added to the micro ELISA plate wells. Human VEGF-A would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human VEGF-A. You can calculate the concentration of Human VEGF-A in the samples by comparing the OD of the samples to the standard curve.

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human VEGF-A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human VEGF-A were tested on 3 different plates, 20 replicates in each plate.

The recovery of Human VEGF-A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Vascular endothelial growth factor A(VEGF-A) is a multifunctional cytokine that exerts in vivo a key role in physiological and pathological neoangiogenesis by stimulating endothelial cell proliferation and vessel hyperpermeability.[1] It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and encodes a protein that is often found as a disulfide linked homodimer. In human, VEGF-A is produced by a group of isoforms as a result of alternative splicing and VEGF189, VEGF165, VEGF121 are widely expressed. Elevated levels of this protein is linked to POEMS syndrome, mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.[2] VEGF-A also has a major role in the pathogenesis of many other diseases including hypervascularized tumors, rheumatoid arthritis, cutaneous diseases. [3]

1. Murphy J F , Fitzgerald D J . Vascular endothelial growth factor induces cyclooxygenase-dependent proliferation of endothelial cells via the VEGF-2 receptor[J]. The FASEB Journal, 2001, 15(9):1667-1669.

2. Watanabe D, Suzuma K, Suzuma I, et al. Vitreous levels of angiopoietin 2 and vascular endothelial growth factor in patients with proliferative diabetic retinopathy[J]. American journal of ophthalmology, 2005, 139(3): 476-481.