QuicKey Mouse IgG(Immunoglobulin G) ELISA Kit

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This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG. Samples (or Standards) and biotinylated detection antibody specific for Mouse IgG are added to the micro ELISA plate wells. Mouse IgG would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Mouse IgG. You can calculate the concentration of Mouse IgG in the samples by comparing the OD of the samples to the standard curve.

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IgG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IgG were tested on 3 different plates, 20 replicates in each plate.

The recovery of Mouse IgG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Immunoglobulin G (IgG) is a class of immunoglobulins found in all body fluids. They are the smallest but most common antibodies (75 percent to 80 percent) in the body. IgG antibodies are large molecules of about 150 kDa[1], made of four peptide chains. It contains two identical class γ heavy chains of about 50 kDa and two identical light chains of about 25 kDa, thus a tetrameric quaternary structure. IgG antibodies are very important in fighting bacterial and viral infections, and they are the only type of antibody that can cross the placenta in a pregnant woman to help the fetus. The measurement of immunoglobulin G can be a diagnostic tool for certain conditions, such as autoimmune hepatitis, if indicated by certain symptoms. Clinically, measured IgG antibody levels are generally considered to be indicative of an individual's immune status to particular pathogens. A common example of this practice are titers drawn to demonstrate serologic immunity to measles, mumps, and rubella (MMR), hepatitis B virus, and varicella (chickenpox) [2].

1. Janeway Jr C A, Travers P, Walport M, et al. Principles of innate and adaptive immunity[M]//Immunobiology: The Immune System in Health and Disease. 5th edition. Garland Science, 2001.

2. Faruque A A, Maoya J, Bisher K D. Analysis of surface plasmon resonance biosensors for protein detection[D]. BRAC University, 2016.