Current Position:Home>>Hot>>QuicKey Pro ELISA>> QuicKey Pro Human AMH(Anti-Mullerian Hormone) ELISA Kit

QuicKey Pro Human AMH(Anti-Mullerian Hormone) ELISA Kit

Citations(6)Uniprot : P03971

Cat.No.:E-OSEL-H0004
Reactivity: Human

Filter:

To Purchase E-OSEL-H0004

Size:

96T
48T
24T
96T*5
96T*10

Price: $520

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

QuicKey Pro Series

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T/24
Assay time	1.5h
Detection range	78.13-5000 pg/mL
Sensitivity	46.88 pg/mL
Sample type &Sample volume	serum and plasma; 50μL
Specificity	This kit recognizes AMH in samples. No significant cross-reactivity or interference between AMH and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of AMH concentrations in serum and plasma.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AMH. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human AMH are added to the micro ELISA plate wells. Human AMH in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to AMH. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human AMH in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T	2-8℃, 1 months
Reference Standard	96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials	2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×)	96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL	2-8℃(Protect from light)
Reference Standard & Sample Diluent	96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL	2-8℃
HRP Linked Ab Diluent	96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×)	96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL
Substrate Reagent	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃(Protect from light)
Stop Solution	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃
Plate Sealer	96T/48T/24T: 5 pieces 96T*5: 25 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level AMH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level AMH were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	249.73	653.82	2390.54	261.16	616.25	2312.39
Standard deviation	16.71	28.64	114.51	16.32	28.16	116.78
CV (%)	6.69	4.38	4.79	6.25	4.57	5.05

Recovery

The recovery of AMH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	83-96	90
EDTA plasma(n=8)	91-106	96

Linearity

Samples were spiked with high concentrations of Human AMH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P03971
Synonyms	AMH，Anti-Mullerian Hormone
Research Area	Cancer, Developmental biology, Signal transduction, Stem cells

Assay Procedures

Citations

European Journal of Obstetrics & Gynecology and Reproductive Biology (2016) IF: 1.662

Serum anti-Müllerian hormone levels in euthyroid adolescent girls with Hashimoto's thyroiditis: relationship to antioxidant status

DOI: 10.1016/j.ejogrb.2016.06.002
	Sample: Serum

European Journal of Obstetrics & Gynecology and Reproductive Biology (2017) IF: 1.666

Diminished ovarian reserve in women with transfusion-dependent beta-thalassemia major: Is iron gonadotoxic?

DOI: 10.1016/j.ejogrb.2017.06.038
PMID: 28732253	Sample: Serum

International Journal of General Medicine (2021) IF: 2.466

Correlation of Serum Anti-Mullerian Hormone (AMH) Level on Ovarian Volume in Women with Endometrioma

DOI: 10.2147/IJGM.S272071
	Sample: Serum

TOXICOLOGY LETTERS (2020) IF: 3.569

Possible role of n-hexane as an endocrine disruptor in occupationally exposed women at reproductive age

DOI: 10.1016/j.toxlet.2020.04.022
	Sample: Serum

TOXICOLOGY AND APPLIED PHARMACOLOGY (2019) IF: 3.585

High copper levels in follicular fluid affect follicle development in polycystic ovary syndrome patients: Population-based and in vitro studies

DOI: 10.1016/j.taap.2019.01.008
PMID: 30641075	Sample: Serum,Cell Culture Medium

ENDOCRINOLOGY (2022) IF: 4.736

Scream sound induced chronic psychological stress results in diminished ovarian reserve in adult female rat

DOI: 10.1210/endocr/bqac042
	Sample: Serum

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha