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QuicKey Pro Human E2(Estradiol) ELISA Kit

Citations(10)

Cat.No.:E-OSEL-H0005
Reactivity: Human

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96T
48T
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96T*5
96T*10

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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T/24
Assay time	1.5h
Detection range	31.25-2000 pg/mL
Sensitivity	13.22 pg/mL
Sample type &Sample volume	serum, plasma, urine, saliva; 50μL
Specificity	This kit recognizes E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of E2 concentrations in serum, plasma, urine, saliva.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human E2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human E2 are added to the micro ELISA plate wells. Human E2 in samples (or standards) competes with a fixed amount of E2 on the solid phase supporter for sites on the HRP linked detection antibody specific to E2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T	2-8℃, 1 months
Reference Standard	96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials	2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×)	96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL	2-8℃(Protect from light)
Reference Standard & Sample Diluent	96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL	2-8℃
HRP Linked Ab Diluent	96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×)	96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL
Substrate Reagent	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃(Protect from light)
Stop Solution	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃
Plate Sealer	96T/48T/24T: 5 pieces 96T*5: 25 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level E2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level E2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	9.73	25.19	80.21	10.31	25.61	86.43
Standard deviation	0.51	1.26	4.24	0.68	1.19	4.61
CV (%)	5.26	4.99	5.29	6.57	4.66	5.33

Recovery

The recovery of E2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	87-98	92
EDTA plasma(n=8)	86-101	93
Urine(n=8)	93-107	99

Linearity

Samples were spiked with high concentrations of Human E2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	Oestradiol; E2; 17β-Estradiol; Estra-1,3,5(10)-triene-3,17β-diol
Research Area	Cell biology, Metabolism

Assay Procedures

Citations

Advanced Science Letters (2018)

Phytoestrogen Intake Correlate with Blood Pressure in Minangkabau Premenopausal People

DOI: 10.1166/asl.2018.12687
	Sample: Serum

JOURNAL OF APPLIED TOXICOLOGY (2019) IF: 3.065

Estrogenic effects of fluorinated diiodine alkanes in MCF‐7 cells, H295R cells and zebrafish embryo assays

DOI: 10.1002/jat.3783
	Sample: Cell Culture Medium

TOXICOLOGY AND APPLIED PHARMACOLOGY (2019) IF: 3.585

High copper levels in follicular fluid affect follicle development in polycystic ovary syndrome patients: Population-based and in vitro studies

DOI: 10.1016/j.taap.2019.01.008
PMID: 30641075	Sample: Serum,Cell Culture Medium

REPRODUCTION (2021) IF: 3.906

Melatonin ameliorates ovarian dysfunction by regulating autophagy in PCOS via the PI3K-Akt pathway

DOI: 10.1530/REP-20-0643
PMID: 33989172	Sample: Serum

TOXICOLOGY LETTERS (2021) IF: 4.374

Copper exposure disrupts ovarian steroidogenesis in human ovarian granulosa cells via the FSHR/CYP19A1 pathway and alters methylation patterns on the SF-1 gene promoter

DOI: 10.1016/j.toxlet.2021.12.002
PMID: 34871762	Sample: Ovarian Granulosa Cell

Scientific Reports (2021) IF: 4.38

Obesity-related proteins score as a potential marker of breast cancer risk

DOI: 10.1038/s41598-021-87583-3
PMID: 33859244	Sample: Plasma

Scientific Reports (2021) IF: 4.38

Obesity-related proteins score as a potential marker of breast cancer risk

DOI: 10.1038/s41598-021-87583-3
PMID: 33859244	Sample: Plasma

EXPERIMENTAL AND MOLECULAR MEDICINE (2020) IF: 8.718

Melatonin stimulates aromatase expression and estradiol production in human granulosa-lutein cells: relevance for high serum estradiol levels in patients with ovarian hyperstimulation syndrome

DOI: 10.1038/s12276-020-00491-w
PMID: 32855437	Sample: Cell Lysate

JOURNAL OF HAZARDOUS MATERIALS (2020) IF: 9.038

Risk assessment of the lipid metabolism-disrupting effects of nitro-PAHs

DOI: 10.1016/j.jhazmat.2020.122611
	Sample: Hepg2 Cell

JOURNAL OF HAZARDOUS MATERIALS (2021) IF: 10.588

New insights into potential estrogen agonistic activity of triazole fungicides and coupled metabolic disturbance

DOI: 10.1016/j.jhazmat.2021.127479
PMID: 34688002	Sample: Hepg2 Cell

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