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QuicKey Pro Human Pg(Progesterone) ELISA Kit

  • Cat.No.:E-OSEL-H0011

  • Reactivity: Human

To Purchase E-OSEL-H0011

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $520
Qty:

QuicKey Pro Series

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T/24
Assay time 1.5h
Detection range 78.13-5000 pg/mL
Sensitivity 36.24 pg/mL
Sample type &Sample volume serum and plasma; 50μL
Specificity This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum and plasma.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human Pg are added to the micro ELISA plate wells. Human Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human Pg in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
24T: 8 wells ×3 strips
96T*5: 5 plates, 96T
2-8℃, 1 months
Reference Standard 96T: 2 vials
48T/24T: 1 vial
96T*5: 10 vials
2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×) 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL
96T*5: 5 vials, 60 μL
2-8℃(Protect from light)
Reference Standard & Sample Diluent 96T/48T/24T: 1 vial, 20 mL
96T*5: 5 vials, 20 mL
2-8℃
HRP Linked Ab Diluent 96T/48T/24T: 1 vial, 14 mL
96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×) 96T/48T/24T: 1 vial, 30 mL
96T*5: 5 vials, 30 mL
Substrate Reagent 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL
2-8℃(Protect from light)
Stop Solution 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL
2-8℃
Plate Sealer 96T/48T/24T: 5 pieces
96T*5: 25 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Pg were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Pg were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 247.69 769.94 1676.10 229.30 733.82 1806.27
Standard deviation 13.82 43.27 62.52 12.86 35.66 65.39
CV (%) 5.58 5.62 3.73 5.61 4.86 3.62

Recovery

The recovery of Pg spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-103 95
EDTA plasma(n=8) 96-111 102

Linearity

Samples were spiked with high concentrations of Human Pg and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsProgesterone,Pg
Research AreaSignal Transduction

Assay Procedures

Citations

  1. Journal of Extracellular Vesicles (2020) IF: 14.976
    Study of immune-tolerized cell lines and extracellular vesicles inductive environment promoting continuous expression and secretion of HLA-G from semiallograft immune tolerance during pregnancy

    DOI: 10.1080/20013078.2020.1795364

    Sample: Bewo Cell
  2. TOXICOLOGY IN VITRO (2021) IF: 3.5
    Long-term in vitro exposure of human granulosa cells to the mixture of endocrine disrupting chemicals found in human follicular fluid disrupts steroidogenesis

    DOI: 10.1016/j.tiv.2021.105302

    PMID: 34929288

    Sample: Hgrc1 Cell
  3. JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2022) IF: 4.054
    Human umbilical cord mesenchymal stem cell-derived exosomes inhibit ovarian granulosa cells inflammatory response through inhibition of NF-κB signaling in polycystic ovary syndrome

    DOI: 10.1016/j.jri.2022.103638

    PMID: 35588629

    Sample: Granulosa Cell.
  4. JOURNAL OF BIOLOGICAL CHEMISTRY (2016) IF: 4.258
    Activation of PPARγ and CD36 expression--the dual pathophysiological roles of progesterone

    DOI: 10.1074/jbc.M116.726737

    Sample: Serum
  5. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Evaluation of possible effects of Persea americana seeds on female reproductive hormonal and toxicity profile

    DOI: 10.1016/j.jep.2021.113870

    PMID: 33484907

    Sample: Serum
  6. TOXICOLOGY LETTERS (2021) IF: 4.374
    Copper exposure disrupts ovarian steroidogenesis in human ovarian granulosa cells via the FSHR/CYP19A1 pathway and alters methylation patterns on the SF-1 gene promoter

    DOI: 10.1016/j.toxlet.2021.12.002

    PMID: 34871762

    Sample: Ovarian Granulosa Cell
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