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QuicKey Pro Human T(Testosterone)ELISA Kit

  • Cat.No.:E-OSEL-H0007

  • Reactivity: Human

To Purchase E-OSEL-H0007

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $520
Qty:

QuicKey Pro Series

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T/24
Assay time 1.5h
Detection range 0.63-40 ng/mL
Sensitivity 0.24 ng/mL
Sample type &Sample volume serum, plasma, urine, saliva; 50μL
Specificity This kit recognizes T in samples. No significant cross-reactivity or interference between T and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of T concentrations in serum, plasma, urine, saliva.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human T are added to the micro ELISA plate wells. Human T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human T in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
24T: 8 wells ×3 strips
96T*5: 5 plates, 96T
2-8℃, 1 months
Reference Standard 96T: 2 vials
48T/24T: 1 vial
96T*5: 10 vials
2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×) 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL
96T*5: 5 vials, 60 μL
2-8℃(Protect from light)
Reference Standard & Sample Diluent 96T/48T/24T: 1 vial, 20 mL
96T*5: 5 vials, 20 mL
2-8℃
HRP Linked Ab Diluent 96T/48T/24T: 1 vial, 14 mL
96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×) 96T/48T/24T: 1 vial, 30 mL
96T*5: 5 vials, 30 mL
Substrate Reagent 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL
2-8℃(Protect from light)
Stop Solution 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL
2-8℃
Plate Sealer 96T/48T/24T: 5 pieces
96T*5: 25 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level T were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level T were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 2.04 4.88 18.80 2.01 4.96 18.17
Standard deviation 0.14 0.24 0.80 0.12 0.25 0.80
CV (%) 6.63 4.93 4.27 6.08 5.07 4.38

Recovery

The recovery of T spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-99 91
EDTA plasma(n=8) 94-106 100
Urine(n=8) 93-109 99

Linearity

Samples were spiked with high concentrations of Human T and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Assay Procedures

Citations

  1. HIGH ALTITUDE MEDICINE & BIOLOGY (2021) IF: 1.981
    Association Between 17β-Estradiol Receptors and Nitric Oxide Signaling Augments High-Altitude Adaptation of Ladakhi Highlanders

    DOI: 10.1089/ham.2020.0187

    Sample: Plasma
  2. Reproductive Biology (2022) IF: 2.089
    ALG2 inhibits the epithelial-to-mesenchymal transition and stemness of ovarian granulosa cells through the Wnt/β-catenin signaling pathway in polycystic ovary syndrome

    DOI: 10.1016/j.repbio.2022.100706

    PMID: 36327672

    Sample: Serum
  3. ANDROLOGIA (2022) IF: 2.532
    The impact of dolutegravir-based combination antiretroviral therapy on the spermatozoa and fertility parameters of men living with human immunodeficiency virus

    DOI: 10.1111/and.14621

    PMID: 36261884

    Sample: Serum
  4. NITRIC OXIDE-BIOLOGY AND CHEMISTRY (2020) IF: 3.311
    Estrogen receptor (ESR1 and ESR2)-mediated activation of eNOS–NO–cGMP pathway facilitates high altitude acclimatization

    DOI: 10.1016/j.niox.2020.05.003

    Sample: Plasma
  5. TOXICOLOGY LETTERS (2019) IF: 3.499
    Cholestasis-associated reproductive toxicity in male and female rats: The fundamental role of mitochondrial impairment and oxidative stress

    DOI: 10.1016/j.toxlet.2019.09.009

    Sample: Serum
  6. TOXICOLOGY AND APPLIED PHARMACOLOGY (2019) IF: 3.585
    High copper levels in follicular fluid affect follicle development in polycystic ovary syndrome patients: Population-based and in vitro studies

    DOI: 10.1016/j.taap.2019.01.008

    PMID: 30641075

    Sample: Serum,Cell Culture Medium
  7. REPRODUCTION (2021) IF: 3.906
    Melatonin ameliorates ovarian dysfunction by regulating autophagy in PCOS via the PI3K-Akt pathway

    DOI: 10.1530/REP-20-0643

    PMID: 33989172

    Sample: Serum
  8. TOXICOLOGY LETTERS (2021) IF: 4.374
    Copper exposure disrupts ovarian steroidogenesis in human ovarian granulosa cells via the FSHR/CYP19A1 pathway and alters methylation patterns on the SF-1 gene promoter

    DOI: 10.1016/j.toxlet.2021.12.002

    PMID: 34871762

    Sample: Ovarian Granulosa Cell
  9. Frontiers in Physiology (2021) IF: 4.566
    Human Physiological Responses to a Single Deep Helium-Oxygen Diving

    DOI: 10.3389/fphys.2021.735986

    Sample: Saliva
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