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Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Green)

Citations(76)

  • Cat.No.:E-BC-K138-F

  • Detection instrument: Fluorescence Microplate reader/Fluorescence Spectrophotometer /Flow Cytometry(Ex/Em=500 nm/525 nm)

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Detection principle

DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.

Performance characteristics

Synonyms ROS, DCFH-DA, DCFH, DCF
Sample type Fresh tissue,cultured cells
Detection method Fluorescence method
Assay type Cell-based (quantitative)
Assay time 60 min
Other instruments required Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge
Storage Reagent 1: -20℃, Reagent 2: 2-8℃
Valid period 12 months

Images

ROS level in mouse heart was significantly increased in Ctrl group and LST group, and reversed in HST, CDDP and CST groups. (***P<0.001, ###P<0.001)

YF Yang et al investigate the function of Danshen Dripping Pill in inhibiting heart failure. Reactive oxygen species (ROS) in mouse heart was determined using ROS fluorometric assay kit (E-BC-K138-F).

Citations

  1. BIOORGANIC CHEMISTRY (2020) IF: 4.831
    Two resveratrol analogs, pinosylvin and 4,4′-dihydroxystilbene, improve oligoasthenospermia in a mouse model by attenuating oxidative stress via the Nrf2-ARE pathway

    DOI: 10.1016/j.bioorg.2020.104295
    Sample: Testicular
  2. JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM (2021) IF: 6.2
    Nrf2-BDNF-TrkB pathway contributes to cortical hemorrhage-induced depression, but not sex differences:

    DOI: 10.1177/0271678X211029060
    Sample: Brain
  3. PLoS Genetics (2021) IF: 5.917
    Patient-derived iPSCs link elevated mitochondrial respiratory complex I function to osteosarcoma in Rothmund-Thomson syndrome

    DOI: 10.1371/journal.pgen.1009971

    PMID: 34965247

    		 Sample: Rts Fibroblast,Fc Fibroblast
  4. Arabian Journal of Chemistry (2022) IF: 5.165
    Structural analysis and immunomodulatory activity of a homopolysaccharide isolated from Parabacteroides distasonis

    DOI: 10.1016/j.arabjc.2022.103755
    Sample: Raw 264.7 Cell
  5. JOURNAL OF ETHNOPHARMACOLOGY (2023) IF: 5.195
    Verbenalin attenuates hepatic damage and mitochondrial dysfunction in alcohol-associated steatohepatitis by regulating MDMX/PPARα-mediated ferroptosis

    DOI: 10.1016/j.jep.2023.116227
    Sample: Serum,Liver Tissue,Aml-12 Cell
  6. BIOFACTORS (2022) IF: 6.113
    Wnt inhibitory 1 ameliorates neovascularization and attenuates photoreceptor injury in an oxygen-induced retinopathy mouse model

    DOI: 10.1002/biof.1824

    PMID: 35080047

    		 Sample: Retinal Cell
  7. Frontiers in Molecular Biosciences (2022) IF: 6.113
    Glucose Deprivation Induced by Acarbose and Oncolytic Newcastle Disease Virus Promote Metabolic Oxidative Stress and Cell Death in a Breast Cancer Model

    DOI: 10.3389/fmolb.2022.816510

    PMID: 35936786

    		 Sample: Mammary Adenocarcinoma Tissue
  8. Molecular & Cellular Toxicology (2022) IF: 1.08
    Normobaric hyperoxia re-sensitizes paclitaxel-resistant lung cancer cells

    DOI: 10.1007/s13273-022-00225-1
    Sample: A549 Cell
  9. Bratislava Medical Journal-Bratislavske Lekarske Listy (2023) IF: 1.564
    Choline and citicoline ameliorate oxidative stress in acute kidney injury in rats.

    DOI: 10.4149/bll_2023_007
    Sample: Kidney Tissue
  10. INTERNATIONAL JOURNAL OF PHOTOENERGY (2021) IF: 2.113
    Experimental Study of the Effect of Photobiomodulation Therapy on the Regulation of the Healing Process of Chronic Wounds

    DOI: 10.1155/2021/3947895
    Sample: Serum
  11. BIOLOGICAL & PHARMACEUTICAL BULLETIN (2022) IF: 2.264
    Ajania purpurea Extract Attenuates LPS-Induced Inflammation in RAW264.7 Cells and Peritonitis Mice

    DOI: 10.1248/bpb.b22-00388
  12. Experimental and Therapeutic Medicine (2021) IF: 2.447
    Zhike pingchuan granules improve bronchial asthma by regulating the IL‑6/JAK2/STAT3 pathway

    DOI: 10.3892/etm.2021.10331
    Sample: Lung
  13. Experimental and Therapeutic Medicine (2021) IF: 2.447
    Pitavastatin maintains MAPK7 expression and alleviates angiotensin II‑induced vascular endothelial cell inflammation and injury

    DOI: 10.3892/etm.2021.11055
    Sample: Huvec
  14. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    Zanthoxylum bungeanum Seed Oil Attenuates LPS-Induced BEAS-2B Cell Activation and Inflammation by Inhibiting the TLR4/MyD88/NF-κB Signaling Pathway

    DOI: 10.1155/2021/2073296
    Sample: Beas-2B Cell
  15. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    Main Active Components and Cell Cycle Regulation Mechanism of Astragali Radix and Angelicae Sinensis Radix in the Treatment of Ox-LDL-Induced HUVECs Injury and Inhibition of Their Cell Cycle

    DOI: 10.1155/2021/8087183

    PMID: 34471419

    		 Sample: Huvecs
  16. JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2020) IF: 2.644
    Quercetin protects against experimentally-induced myocardial infarction in rats by an antioxidant potential and concomitant activation of signal transducer and activator of transcription 3

    DOI: 10.26402/jpp.2020.6.11
    Sample: Left Ventricles
  17. Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2022) IF: 2.693
    Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains

    DOI: 10.1002/jez.2603
    Sample: Cortex
  18. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY (2020) IF: 2.734
    Protective effects of 1,2-benzisoxazole-3-methanesulfonamide (zonisamide)- loaded polymeric micelles against neurotoxicity in spinal cord: in vitro

    DOI: 10.1016/j.jddst.2020.102311
    Sample: Cell Lysate
  19. SAUDI PHARMACEUTICAL JOURNAL (2020) IF: 2.879
    Fisetin protects against streptozotocin-induced diabetic cardiomyopathy in rats by suppressing fatty acid oxidation and inhibiting protein kinase R

    DOI: 10.1016/j.jsps.2020.12.003

    PMID: 33603537

    		 Sample: Cell Lysate
  20. NEUROSCIENCE LETTERS (2021) IF: 3.046
    Chlorogenic acid alleviates neurobehavioral disorders and brain damage in focal ischemia animal models

    DOI: 10.1016/j.neulet.2021.136085

    PMID: 34174343

    		 Sample: Cerebral Cortical
  21. NEUROSCIENCE LETTERS (2022) IF: 3.046
    Chlorogenic acid alleviates cerebral ischemia-induced neuroinflammation via attenuating nuclear factor kappa B activation

    DOI: 10.1016/j.neulet.2022.136495

    PMID: 35108588

    		 Sample: Cerebral Cortex Tissue
  22. Bioengineered (2022) IF: 3.269
    Liraglutide inhibits receptor for advanced glycation end products (RAGE)/reduced form of nicotinamide-adenine dinucleotide phosphate (NAPDH) signaling to ameliorate non-alcoholic fatty liver disease (NAFLD) in vivo and vitro

    DOI: 10.1080/21655979.2022.2036902

    PMID: 35164657

    		 Sample: Aml12 Cell
  23. Bioengineered (2022) IF: 3.269
    Amentoflavone promotes ferroptosis by regulating reactive oxygen species (ROS) /5’AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) to inhibit the malignant progression of endometrial carcinoma cells

    DOI: 10.1080/21655979.2022.2079256
    Sample: Kle Cell
  24. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2023) IF: 3.322
    The mitochondria-targeted small molecule SS31 delays progression of behavioral deficits by attenuating β-amyloid plaque formation and mitochondrial/synaptic deterioration in APP/PS1 mice

    DOI: 10.1016/j.bbrc.2023.02.076
    Sample: Hippocampal
  25. DISEASE MARKERS (2022) IF: 3.434
    Effect of HIV-1 Protease Inhibitor on IL-18 and IL-1β in Rats with Insulinoma

    DOI: 10.1155/2022/1868749
    Sample: Hippocampus Tissue
  26. Frontiers in Veterinary Science (2022) IF: 3.471
    11S Glycinin Up-Regulated NLRP-3-Induced Pyroptosis by Triggering Reactive Oxygen Species in Porcine Intestinal Epithelial Cells

    DOI: 10.3389/fvets.2022.890978

    PMID: 35782549

    		 Sample: Ipec-J2 Cell
  27. PHARMACEUTICAL BIOLOGY (2021) IF: 3.503
    The anti-breast cancer property of physcion via oxidative stress-mediated mitochondrial apoptosis and immune response

    DOI: 10.1080/13880209.2021.1889002

    PMID: 33715588

    		 Sample: Serum
  28. ORAL DISEASES (2022) IF: 3.511
    Periostin regulates LPS-induced apoptosis via Nrf2/HO-1 pathway in periodontal ligament fibroblasts

    DOI: 10.1111/odi.14189

    PMID: 35298860

    		 Sample: Pdlfs
  29. ACS Omega (2022) IF: 3.512
    Specific Tumor Cell Detection by a Metabolically Targeted Aggregation-Induced Emission-Based Gold Nanoprobe

    DOI: 10.1021/acsomega.2c01494
    Sample: Mcf-7 Cell,Hepg2 Cell,Caco-2 Cell
  30. Frontiers in Behavioral Neuroscience (2022) IF: 3.558
    Acupuncture Relieves Stress-Induced Depressive Behavior by Reducing Oxidative Stress and Neuroapoptosis in Rats

    DOI: 10.3389/fnbeh.2021.783056

    PMID: 35058758

    		 Sample: Hippocampus Tissue
  31. METABOLIC BRAIN DISEASE (2022) IF: 3.584
    Hepatoprotective and neuroprotective effect of taxifolin on hepatic encephalopathy in rats

    DOI: 10.1007/s11011-022-00952-3

    PMID: 35298730

    		 Sample: Hippocampal,Hepatic
  32. LIFE SCIENCES (2020) IF: 3.647
    Quercetin attenuated oxidative DNA damage through NRF2 signaling pathway in rats with DMH induced colon carcinogenesis

    DOI: 10.1016/j.lfs.2020.117584

    PMID: 32220623

    		 Sample: Tissue Homogenate
  33. LIFE SCIENCES (2020) IF: 3.647
    Attenuation of chronic arsenic neurotoxicity via melatonin in male offspring of maternal rats exposed to arsenic during conception: Involvement of oxidative DNA damage and inflammatory signaling cascades

    DOI: 10.1016/j.lfs.2020.118876

    PMID: 33310035

    		 Sample: Cell
  34. NEUROCHEMICAL RESEARCH (2021) IF: 3.996
    Epigallocatechin Gallate Alleviates Down-Regulation of Thioredoxin in Ischemic Brain Damage and Glutamate-Exposed Neuron

    DOI: 10.1007/s11064-021-03403-0

    PMID: 34327632
  35. CARDIOVASCULAR DRUGS AND THERAPY (2020) IF: 4.069
    Exendin-4 Attenuates Remodeling in the Remote Myocardium of Rats After an Acute Myocardial Infarction by Activating β-Arrestin-2, Protein Phosphatase 2A, and Glycogen Synthase Kinase-3 and Inhibiting β-Catenin

    DOI: 10.1007/s10557-020-07006-9

    PMID: 32474680

    		 Sample: Tissue Homogenate
  36. BIOCHIMIE (2022) IF: 4.079
    Glucose deprivation using 2-deoxyglucose and acarbose induce metabolic oxidative stress and apoptosis in female mice bearing breast cancer

    DOI: 10.1016/j.biochi.2022.01.007

    PMID: 35066100

    		 Sample: Breast Tumor Tissue
  37. TOXICOLOGY (2020) IF: 4.099
    Inhibition of ferroptosis attenuates busulfan-induced oligospermia in mice

    DOI: 10.1016/j.tox.2020.152489
    Sample: Testicular Tissue
  38. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2021) IF: 4.101
    Genotoxicity of chloroacetamide herbicides and their metabolites in vitro and in vivo

    DOI: 10.3892/ijmm.2021.4936
    Sample: Cell Sample
  39. ACS Omega (2023) IF: 4.132
    Terminators or Guardians? Design, Synthesis, and Cytotoxicity Profiling of Chalcone-Sulfonamide Hybrids

    DOI: 10.1021/acsomega.2c07285
    Sample: Hct116 Cell
  40. Drug Design Development and Therapy (2022) IF: 4.162
    Didymin Ameliorates Liver Fibrosis by Alleviating Endoplasmic Reticulum Stress and Glycerophospholipid Metabolism: Based on Transcriptomics and Metabolomics

    DOI: 10.2147/DDDT.S351092

    PMID: 35698653

    		 Sample: Liver
  41. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019) IF: 4.183
    Ionizing Radiation Increases the Activity of Exosomal Secretory Pathway in MCF-7 Human Breast Cancer Cells: A Possible Way to Communicate Resistance against Radiotherapy

    DOI: 10.3390/ijms20153649

    PMID: 31349735

    		 Sample: Cells
  42. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2021) IF: 4.219
    Quercetin prevents myocardial infarction adverse remodeling in rats by attenuating TGF-β1/Smad3 signaling: Different mechanisms of action

    DOI: 10.1016/j.sjbs.2021.02.007

    PMID: 34012318

    		 Sample: Tissue Homogenate(Heart)
  43. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223
    The ameliorative effect of kaempferol against CdCl2- mediated renal damage entails activation of Nrf2 and inhibition of NF-kB

    DOI: 10.1007/s11356-022-19876-7

    PMID: 35355181

    		 Sample: Kidney Homogenates
  44. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) IF: 4.223
    Kaempferol prevents cadmium chloride-induced liver damage by upregulating Nrf2 and suppressing NF-κB and keap1

    DOI: 10.1007/s11356-021-16711-3

    PMID: 34599712

    		 Sample: Liver
  45. Scientific Reports (2021) IF: 4.38
    Inhibition of cardiac PERK signaling promotes peripartum cardiac dysfunction

    DOI: 10.1038/s41598-021-98344-7

    PMID: 34548576

    		 Sample: Cardiomyocyte
  46. MOLECULES (2021) IF: 4.412
    Expression Profiling of Flavonoid Biosynthesis Genes and Secondary Metabolites Accumulation in Populus under Drought Stress

    DOI: 10.3390/molecules26185546

    PMID: 34577017

    		 Sample: Leaf
  47. EUROPEAN JOURNAL OF PHARMACOLOGY (2022) IF: 4.432
    Isovitexin protects against acute liver injury by targeting PTEN, PI3K and BiP via modification of m6A

    DOI: 10.1016/j.ejphar.2022.174749

    PMID: 35007522

    		 Sample: Serum
  48. Journal of Functional Foods (2021) IF: 4.451
    Structural characterization and immunomodulatory activity of a glucan from Radix Codonopsis

    DOI: 10.1016/j.jff.2021.104537
    Sample: Cell Culture Supernatant
  49. Journal of Bone Oncology (2023) IF: 4.491
    Indirect bilirubin impairs invasion of osteosarcoma cells via inhibiting the PI3K/AKT/MMP-2 signaling pathway by suppressing intracellular ROS

    DOI: 10.1016/j.jbo.2023.100472
    Sample: 143B Cell,Mg63 Cell
  50. Frontiers in Physiology (2021) IF: 4.566
    Cigarette Smoke Promotes Interleukin-8 Production in Alveolar Macrophages Through the Reactive Oxygen Species/Stromal Interaction Molecule 1/Ca2+ Axis

    DOI: 10.3389/fphys.2021.733650
    Sample: Lung Tissue
  51. Journal of Inflammation Research (2023) IF: 4.631
    Neutrophil Extracellular Traps Induce Alveolar Macrophage Pyroptosis by Regulating NLRP3 Deubiquitination, Aggravating the Development of Septic Lung Injury

    DOI: 10.2147/JIR.S366436
  52. Aging-US (2020) IF: 4.831
    Melatonin alleviates angiotensin-II-induced cardiac hypertrophy via activating MICU1 pathway

    DOI: 10.18632/aging.202159

    PMID: 33259334

    		 Sample: Primary Cardiomyocytes (Nmvms)
  53. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Luteolin activates Tregs to promote IL-10 expression and alleviating caspase-11-dependent pyroptosis in sepsis-induced lung injury

    DOI: 10.1016/j.intimp.2021.107914

    PMID: 34246059

    		 Sample: Lung
  54. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Isoliquiritigenin alleviates P. gingivalis-LPS/ATP-induced pyroptosis by inhibiting NF-κB/ NLRP3/GSDMD signals in human gingival fibroblasts

    DOI: 10.1016/j.intimp.2021.108338

    PMID: 34794890
  55. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
    Daurisoline alleviated experimental colitis in vivo and in vitro: Involvement of NF-κB and Wnt/β-Catenin pathway

    DOI: 10.1016/j.intimp.2022.108714

    PMID: 35366641

    		 Sample: Raw 264.7 Cell
  56. Scientific Reports (2022) IF: 4.996
    Testosterone promotion effect of Eucommia ulmoides staminate flower via the steroidogenic pathway and potential hormonal mechanism

    DOI: 10.1038/s41598-022-23578-y
  57. Antioxidants (2020) IF: 5.014
    CsCYT75B1, a Citrus CYTOCHROME P450 Gene, Is Involved in Accumulation of Antioxidant Flavonoids and Induces Drought Tolerance in Transgenic Arabidopsis

    DOI: 10.3390/antiox9020161
    Sample: Leaf
  58. CHEMICO-BIOLOGICAL INTERACTIONS (2021) IF: 5.194
    Cytotoxic activity and influence on acetylcholinesterase of series dinuclear platinum(II) complexes with aromatic nitrogen-containing heterocyclic bridging ligands: Insights in the mechanisms of action

    DOI: 10.1016/j.cbi.2021.109708

    PMID: 34666020

    		 Sample: Mrc-5 Cell,A375 Cell,Hela Cell,Panc-1 Cell
  59. BIOORGANIC CHEMISTRY (2021) IF: 5.275
    Discovery of novel tubulin inhibitors targeting the colchicine binding site via virtual screening, structural optimization and antitumor evaluation

    DOI: 10.1016/j.bioorg.2021.105486

    PMID: 34801948
  60. Algal Research-Biomass Biofuels and Bioproducts (2023) IF: 5.276
    Metabolomic analysis reveals the responses of docosahexaenoic-acid-producing Schizochytrium under hyposalinity conditions

    DOI: 10.1016/j.algal.2023.102987
    Sample: Microalgal Cell
  61. BIOORGANIC CHEMISTRY (2023) IF: 5.307
    Inspired by bis-β-carboline alkaloids: Construction and antitumor evaluation of a novel bis-β-carboline scaffold as potent antitumor agents

    DOI: 10.1016/j.bioorg.2023.106401
    Sample: A549 Cell
  62. Food & Function (2022) IF: 5.396
    The rice bran peptide KF-8 extends the lifespan and improves the healthspan of Caenorhabditis elegans via skn-1 and daf-16

    DOI: 10.1039/D1FO03718H

    PMID: 35170608

    		 Sample: L3 Larvae
  63. Journal of the American Heart Association (2022) IF: 5.501
    Vitamin C Improves the Outcomes of Cardiopulmonary Resuscitation and Alters Shedding of Syndecan‐1 and p38/MAPK Phosphorylation in a Rat Model

    DOI: 10.1161/JAHA.121.023787
    Sample: Heart,Brain
  64. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY (2022) IF: 5.652
    The mechanism underlying the TC-G 1008 rescue of reactive oxygen species (ROS)-induced osteoblast apoptosis by the upregulation of peroxiredoxin 1

    DOI: 10.1016/j.biocel.2022.106276

    PMID: 35953014

    		 Sample: Mc3T3-E1 Cell
  65. JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY (2023) IF: 5.756
    Discovery of potent tubulin inhibitors targeting the colchicine binding site via structure-based lead optimization and antitumor evaluation

    DOI: 10.1080/14756366.2022.2155815
    Sample: Mda-Mb-231 Cell
  66. Current Research in Food Science (2022) IF: 6.269
    Proteomic analyses revealed the antibacterial mechanism of Aronia melanocarpa isolated anthocyanins against Escherichia coli O157: H7

    DOI: 10.1016/j.crfs.2022.09.017

    PMID: 36147549
  67. PEDIATRIC ALLERGY AND IMMUNOLOGY (2021) IF: 6.377
    The dispersion and utilization of lipid droplets mediates respiratory syncytial virus-induced airway hyperresponsiveness

    DOI: 10.1111/pai.13651
    Sample: Beas-2B Cell
  68. DRUG DELIVERY (2022) IF: 6.819
    Docetaxel prodrug and hematoporphyrin co-assembled nanoparticles for anti-tumor combination of chemotherapy and photodynamic therapy

    DOI: 10.1080/10717544.2022.2147280
  69. HYPERTENSION (2018) IF: 6.823
    Potential Protective Mechanism in the Cardiac Microvascular Injury

    DOI: 10.1161/HYPERTENSIONAHA.118.11035
    Sample: Cardiac Microvascular Endothelial Cells (Cmecs)
  70. CNS Neuroscience & Therapeutics (2023) IF: 7.035
    Alpinetin inhibits neuroinflammation and neuronal apoptosis via targeting the JAK2/STAT3 signaling pathway in spinal cord injury

    DOI: 10.1111/cns.14085
    Sample: Pc12 Cell
  71. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022) IF: 7.129
    Parental cadmium exposure during the spawning period reduces cadmium sensitivity through the antioxidant system in rare minnow (Gobiocypris rarus) larvae

    DOI: 10.1016/j.ecoenv.2022.114137

    PMID: 36201919

    		 Sample: Larvae
  72. Microbiology Spectrum (2021) IF: 7.171
    High-Fat-Diet–Induced Oxidative Stress Linked to the Increased Colonization of Lactobacillus sakei in an Obese Population | Microbiology Spectrum

    DOI: 10.1128/Spectrum.00074-21
    Sample: Colon Tissue
  73. Antioxidants (2022) IF: 7.675
    Mangosteen Pericarp Extract Supplementation Boosts Antioxidant Status via Rebuilding Gut Microbiota to Attenuate Motor Deficit in 6-OHDA-Induced Parkinson’s Disease

    DOI: 10.3390/antiox11122396
    Sample: Brain
  74. FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 8.101
    Inhibition of ferroptosis attenuates oligospermia in male Nrf2 knockout mice

    DOI: 10.1016/j.freeradbiomed.2022.10.314

    PMID: 36309297

    		 Sample: Seminal Plasma
  75. WATER RESEARCH (2020) IF: 9.13
    Comparative toxicity reduction potential of UV/sodium percarbonate and UV/hydrogen peroxide treatments for bisphenol A in water: An integrated analysis using chemical, computational, biological, and metabolomic approaches

    DOI: 10.1016/j.watres.2020.116755

    PMID: 33383346
  76. Acta Pharmaceutica Sinica B (2022) IF: 14.903
    Compound Danshen Dripping Pill inhibits hypercholesterolemia/atherosclerosis-induced heart failure in ApoE and LDLR dual deficient mice via multiple mechanisms

    DOI: 10.1016/j.apsb.2022.11.012
    Sample: Heart
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Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
Q

Ö*****nSubmitted [ Oct 19 2023 ]

Asked: What should I use Reagent 3 for?

Reply

A

adminSubmitted [ Oct 19 2023 ]

Answered: Reagent 3 can be used to dilute reagents 1 and 2, and can also be used to wash cells. The product has now been updated and is no longer supplied with reagent 3, but is replaced with serum-free medium.

A

adminSubmitted [ Oct 19 2023 ]

Answered: Reagent 3 can be used to dilute reagents 1 and 2, and can also be used to wash cells. The product has now been updated and is no longer supplied with reagent 3, but is replaced with serum-free medium.

Q

s*****MSubmitted [ Aug 22 2023 ]

Asked: What is the use of reagent2 working solution mentioned in kit E-BC-K138-F

Reply

A

adminSubmitted [ Aug 22 2023 ]

Answered: Reagent 2 is a positive control reagent that can be used to set up a positive control well to help determine whether reagent 1 is working properly.

Q

M***********gSubmitted [ Jul 24 2023 ]

Asked: Hi there. I noticed that we can use fluorescence microscopy for this ROS assay. May I know if the black microplate provided in this kit, is it transparent or opaque bottom? And for fluorescence microscopy, do I need to use transparent or opaque bottom black microplate?

Reply

A

adminSubmitted [ Jul 24 2023 ]

Answered: Hi, I'll reply to your mail

Q

B**********dSubmitted [ May 08 2023 ]

Asked: MCF-7 ve MDA-MB 231 breast cancer cell line için ve kanser kök hücreleri kullanılabilir mi? Yapılan çalışma varsa paylaşabilir misin?

Reply

A

adminSubmitted [ May 08 2023 ]

Answered: Thanks for your inquiry!I'll reply to you by email later. Pls pay attention to the mailbox.

Q

M******rSubmitted [ Apr 10 2023 ]

Asked: Hello, I want to perform this protocol but combining it with DAPI staining to measure ROS production in control and drug-treated cells since the drug treatment will reduce the cell number and it will lead to a clouded data. Is it possible to stain cells with DAPI after performing this assay?

Reply

A

adminSubmitted [ Apr 10 2023 ]

Answered: If you use flow cytometry, it is possible to stain cells with DAPI after performing this assay theoretically.

Q

M******rSubmitted [ Feb 28 2023 ]

Asked: Could you please forward the assay protocol? I could not find it. Thanks in advance

Reply

A

adminSubmitted [ Feb 28 2023 ]

Answered: Hi, Mervenur. Well noted! I'll send it to you by email later. Pls pay attention to the mailbox

Q

F******** Submitted [ Nov 30 2022 ]

Asked: Can this assay kit be used for fluorescence microscope acquisition of ROS fluorescence intensity of adherent cells?

Reply

A

adminSubmitted [ Nov 30 2022 ]

Answered: Yes,this kit is available for fluorescence microplate reader, fluorescence microscope and flow cytomet

Q

M*****aSubmitted [ Oct 14 2022 ]

Asked: Would you recommend this kit to quantify the content of ROS in mouse plasma? If not, is there an alternative kit that you would recommend?

Reply

A

adminSubmitted [ Oct 14 2022 ]

Answered: Our ROS kit can only measure living cells, but not for serum plasma. Other relevant indicators can be considered, such as SOD,MDA,GPX, etc

Q

H*********rSubmitted [ Oct 09 2022 ]

Asked: Merhaba bu kitin protokolünü bulamadım yardımci ola bilirmisiniz acaba?

Reply

A

adminSubmitted [ Oct 09 2022 ]

Answered: Dear Hanım, I have send you the manual by email.

Q

M*****aSubmitted [ Sep 16 2022 ]

Asked: could you please provide information about the sensitivity of your \"Reactive Oxygen Species (ROS) Fluorometric Assay Kit\" (#E-BC-K138)? I was not able to find the data in the manual.

Reply

A

adminSubmitted [ Sep 16 2022 ]

Answered: This kit has no sensitivity and measures relative content.

Q

S********** Submitted [ Aug 07 2022 ]

Asked: Can we use this kit for Blood samples (Red Blood Cells) ROS detection ?

Reply

A

adminSubmitted [ Aug 07 2022 ]

Answered: Sorry, it can not use for Blood sample.

Q

a********dSubmitted [ Apr 06 2021 ]

Asked: What is the procedure for this assay?

Reply

A

adminSubmitted [ Apr 06 2021 ]

Answered: Hi, Thanks for your interests in our products. There are detailed operation in the manual. Can we know where didn't we make clear? If you need any help, pls feel free to ask. Best regards, Hanny

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