Synonyms	Pro-Epidermal Growth Factor;EGF;Epidermal Growth Factor;Urogastrone
Species	Human
Expression_host	E.coli
Sequence	Asn971-Arg1023
Accession	P01133
Application	Cell Culture
Mol_Mass	7.2 kDa
AP_Mol_Mass	9-11 kDa
Tag	C-His
Bio_Activity	Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is < 1.0 ng/mL. The specific activity of recombinant human EGF is approximately >1.0 x 10⁶ IU/mg.

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 0.1 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites.

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