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SARS-CoV-2 Neutralization Antibody ELISA Kit

  • Cat.No.:E-EL-E606

  • Reactivity: Human
  • Assay Type: One step Competitive-ELISA
  • Assay time: 1.5h

To Purchase E-EL-E606

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $576


Format 24T, 96T, 96T*5, 96T*10
Assay time 1.5h
Sample type serum and plasma
Sample volume 50 μL
Storage 2-8℃
Interpretation Qualitative
Application This ELISA kit applies to the in vitro Qualitative determination of SARS-CoV-2 Neutralization Antibody in serum or plasma.
Reproducibility Both intra-CV and inter-CV are < 15%
Synonyms COVID 19 Neutralization Antibody, Anti-RBD, RBD Antibody, S1RBD Antibody

Test Principle

This ELISA kit uses Competitive-ELISA as the method to qualitatively detect the Anti-SARS-CoV-2 Neutralization Antibody in the sample. _x000D_ The micro ELISA plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 Neutralization Antibody in the pretreated samples or controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD). After 37℃ incubation, the unbound HRP-RBD as well as any HRP-RBD bound to non-Neutralization antibody will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRP-RBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. Compared with the inhibition ratio to judge whether SARS-CoV-2 Neutralization Antibody exists in the tested samples or not.

Assay Procedures


  1. Cells (2022) IF: 6.6
    Venetoclax Decreases the Expression of the Spike Protein through Amino Acids Q493 and S494 in SARS-CoV-2

    DOI: 10.3390/cells11121924

    PMID: 35741053

    Sample: Hek293T Cell
  2. Healthcare (2023) IF: 3.16
    Evaluation of Convalescent Plasma in the Management of Critically Ill COVID-19 Patients (with No Detectable Neutralizing Antibodies Nab) in Kashmir, India

    DOI: 10.3390/healthcare11030317

    Sample: Plasma
    Randomized clinical trial of BCG vaccine in patients with convalescent COVID-19: Clinical evolution, adverse events, and humoral immune response

    DOI: 10.1111/joim.13523

    PMID: 35599154

    Sample: Plasma
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  • Q&A

J***nSubmitted [ Feb 09 2023 ]

Asked: I want to analyze neutralizing antibodies in mouse blood using this kit. In my opinion, even if it is not a human antibody, it is possible to analyze antibodies of other species against RBD using this kit. Can I use this kit to analyze for mouse serum?



adminSubmitted [ Feb 09 2023 ]

Answered: In theory, this kit is capable of detecting neutralizing antibodies in the serum/plasma of mice and other species. Considering we have not validated positive blood samples from mice, it is recommended that preliminary tests be performed first.

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