This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided
in this kit has been pre-coated with an antibody specific to SARS-CoV-2 S1RBD.
Samples (or Standards) and biotinylated detection antibody specific for SARS-CoV-2
S1RBD are added to the micro ELISA plate wells. SARS-CoV-2 S1RBD would
combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP)
conjugate are added successively to each micro plate well and incubated. Free
components are washed away. The substrate solution is added to each well. Only those
wells that contain SARS-CoV-2 S1RBD, biotinylated detection antibody and
Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is
terminated by the addition of stop solution and the color turns yellow. The optical
density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The
OD value is proportional to the concentration of SARS-CoV-2 S1RBD. You can
calculate the concentration of SARS-CoV-2 S1RBD in the samples by comparing the
OD of the samples to the standard curve.