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Sucrose Fluorometric Assay Kit

Cat.No.:E-BC-F042
Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/590 nm)

Detection method: Fluorescence method

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96T

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Detection principle

Sucrose can be hydrolyzed by sucrase to produce glucose under acidic conditions, which is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of HRP (horse radish peroxidase), hydrogen peroxide reacts with the fluorescent probe to form red fluorescent substance. The sucrose content can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.

Performance characteristics

Sample type	Plant tissue
Sensitivity	0.15 μmol/L
Detection range	0.15-15 μmol/L
Detection method	Fluorescence method
Assay type	Quantitative
Assay time	70 min
Precision	Average inter-assay CV: 6.500%Average intra-assay CV: 2.300%
Other instruments required	Micropipettor, Incubator, Water bath, Centrifuge
Storage	-20℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.15-15 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Corn tissue homogenate	1500
10% Potato tissue homogenate	300-500
10% Tomato tissue homogenate	200-300
10% Macrophanerophytes leaf tissue homogenate	200-400
10% Carrot tissue homogenate	1500
10% Onion tissue homogenate	500-1000
10% Green pepper tissue homogenate	500-1000
10% Bush leaves tissue homogenate	20-50

Note: The diluent is reagent 1 working solution.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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