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Cat.No.:E-EL-0155
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Assay type | Competitive-ELISA |
Format | 96T/48T |
Assay time | 2.0h |
Detection range | 0.31-20 ng/mL |
Sensitivity | 0.17 ng/mL |
Sample type &Sample volume | serum, plasma, urine, saliva; 50μL |
Specificity | This kit recognizes T in samples. No significant cross-reactivity or interference between T and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of T concentrations in serum, plasma, urine, saliva. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃ |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level T were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level T were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 1.33 | 5.22 | 10.88 | 1.54 | 5.73 | 10.02 |
Standard deviation | 0.11 | 0.35 | 0.46 | 0.14 | 0.35 | 0.68 |
CV (%) | 8.13 | 6.72 | 4.27 | 9.23 | 6.18 | 6.75 |
The recovery of T spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 80-90 | 84 |
EDTA plasma(n=8) | 82-94 | 87 |
Urine(n=8) | 85-102 | 95 |
Samples were spiked with high concentrations of T and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
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1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C |
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2. Aspirate and wash the plate for 3 times |
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3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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5. Add 50μL Stop Solution |
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6. Read the plate at 450nm immediately. Calculation of the results |
PMID: 35668950 |
Sample: Serum |
Sample: Serum |
Sample: Serum |
DOI: 10.1530/JME-21-0095 |
|
PMID: 34270447 |
Sample: Serum |
DOI: 10.1159/000457881 |
|
Sample: Serum |
Sample: Serum |
Sample: Serum |
Sample: Serum,Intra-Testicular |
PMID: 36096157 |
Sample: Serum |
DOI: 10.1042/CS20180179 |
|
Sample: Serum |
Sample: Plasmsa |
Sample: Plasma,Placental |
Sample: Plasma |
Sample: Serum |
Sample: Plasma |
Sample: Serum,Tissue Homogenate |
Sample: Serum |
Sample: Serum |
PMID: 34879886 |
Sample: Serum |
Sample: Plasma |
Sample: Serum |
DOI: 10.1111/jfbc.13505 |
|
Sample: Serum,Tissue Homogenate |
DOI: 10.1111/jfbc.13385 |
|
Sample: Tissue Homogenate |
Sample: Plasma |
DOI: 10.1155/2020/6723204 |
|
PMID: 32184896 |
Sample: Serum |
DOI: 10.1111/and.13360 |
|
Sample: Plasma |
DOI: 10.1111/and.13355 |
|
Sample: Serum |
DOI: 10.1111/and.13669 |
|
Sample: Sperm |
Sample: Plasma |
Sample: Plasma |
PMID: 36327672 |
Sample: Serum |
Sample: Tm3 Cell |
PMID: 35299115 |
Sample: Blood |
Sample: Serum |
Sample: Serum,Tissue Homogenate |
PMID: 30214533 |
Sample: Serum |
Sample: Serum |
Sample: Serum |
Sample: Plasma |
DOI: 10.1002/tox.22559 |
|
Sample: Tissue Homogenate |
Sample: Sexual Organ |
DOI: 10.1111/and.14621 |
|
PMID: 36261884 |
Sample: Serum |
Sample: Serum |
PMID: 32725591 |
Sample: Serum |
DOI: 10.1155/2021/9968463 |
|
PMID: 34987599 |
Sample: Serum |
DOI: 10.1002/tox.22768 |
|
Sample: Serum |
DOI: 10.1002/jez.2672 |
|
Sample: Serum |
DOI: 10.1111/jpn.13779 |
|
Sample: Serum |
DOI: 10.1111/jfbc.13615 |
|
PMID: 33491243 |
Sample: Serum |
PMID: 33845263 |
Sample: Serum |
DOI: 10.1111/and.14243 |
|
PMID: 34498746 |
Sample: Serum |
DOI: 10.1111/and.14028 |
|
PMID: 33650701 |
Sample: Serum |
Sample: Cell Culture Supernatant |
Sample: Serum |
DOI: 10.1111/andr.12824 |
|
Sample: Tissue Homogenate |
DOI: 10.1002/jat.3764 |
|
Sample: Serum |
PMID: 30648235 |
Sample: Serum |
Sample: Serum |
PMID: 30431108 |
Sample: Serum |
PMID: 30483787 |
Sample: Serum |
Sample: Serum |
Sample: Serum |
Sample: Serum |
Sample: Serum |
PMID: 32588312 |
Sample: Serum,Tissue Homogenate |
Sample: Serum |
PMID: 32812151 |
Sample: Serum,Tissue Homogenate |
PMID: 34101148 |
Sample: Testicular |
Sample: Serum |
Sample: Plasma |
PMID: 34718101 |
Sample: Serum |
PMID: 35429613 |
Sample: Serum |
DOI: 10.2147/DMSO.S285025 |
|
Sample: Plasma |
Sample: Serum |
Sample: Serum |
PMID: 36202220 |
Sample: Serum |
DOI: 10.1155/2022/4479261 |
|
PMID: 35529080 |
Sample: Serum |
PMID: 30384460 |
Sample: Serum |
Sample: Plasma |
Sample: Plasma |
PMID: 35986948 |
Sample: Serum |
PMID: 35358924 |
Sample: Serum |
Sample: Serum |
Sample: Plasma |
Sample: Serum |
PMID: 31899225 |
Sample: Serum |
Sample: Serum |
Sample: Serum |
Sample: Serum |
PMID: 32199951 |
Sample: Cell Culture Supernatant |
PMID: 30641075 |
Sample: Serum,Cell Culture Medium |
DOI: 10.1002/jbt.22445 |
|
PMID: 31975554 |
Sample: Serum |
Sample: Serum,Tm3 Cell |
DOI: 10.1002/jbt.22741 |
|
Sample: Serum |
PMID: 34363886 |
Sample: Leydig Cells (Lcs) |
Sample: Plasma |
Sample: Serum |
PMID: 31654709 |
Sample: Serum |
DOI: 10.1530/REP-20-0316 |
|
PMID: 33434152 |
Sample: Plasma |
DOI: 10.1530/REP-20-0643 |
|
PMID: 33989172 |
Sample: Serum |
DOI: 10.1530/REP-20-0451 |
|
PMID: 33258800 |
Sample: Serum,Cell Culture Supernatant(Ovarian Granulosa C |
PMID: 35715026 |
Sample: Plasma |
PMID: 30195209 |
Sample: Serum |
PMID: 32488017 |
Sample: Plasma,Tissue Homogenate |
Sample: Plasma |
Sample: Testicular Tissue |
PMID: 33581194 |
Sample: Serum |
Sample: Serum |
PMID: 34828406 |
Sample: Plasma |
PMID: 32758512 |
Sample: Serum,Tissue Homogenate |
Sample: Serum |
PMID: 36219170 |
Sample: Plasma |
DOI: 10.1039/C9FO02554E |
|
PMID: 32441726 |
Sample: Serum |
DOI: 10.3390/ijms20030630 |
|
PMID: 30717178 |
Sample: Tissue Homogenate |
PMID: 34280466 |
Sample: Serum |
PMID: 34595713 |
Sample: Serum |
Sample: Serum |
DOI: 10.1038/srep43475 |
|
Sample: Serum |
Sample: Serum |
PMID: 35594452 |
Sample: Gonads |
PMID: 34229480 |
Sample: Serum,Testis |
PMID: 30543945 |
Sample: Plasma |
PMID: 34302943 |
Sample: Serum |
Sample: Serum |
PMID: 34871762 |
Sample: Ovarian Granulosa Cell |
PMID: 35688844 |
Sample: Serum |
Sample: Serum |
Sample: Plasma |
DOI: 10.2147/IJN.S206561 |
|
Sample: Leydig Cells |
PMID: 30658301 |
Sample: Serum |
Sample: Saliva |
PMID: 33360214 |
Sample: Cell Culture Supernatant |
Sample: Plasma |
DOI: 10.3390/jcm11195584 |
|
PMID: 36233452 |
Sample: Serum |
PMID: 35962055 |
Sample: Serum |
PMID: 36150640 |
Sample: Plasma |
PMID: 35341824 |
Sample: Serum |
PMID: 33577849 |
Sample: Tissue Homogenate |
PMID: 33581723 |
Sample: Serum |
PMID: 34753504 |
Sample: Serum |
PMID: 35597951 |
Sample: Serum |
DOI: 10.1038/srep21776 |
|
Sample: Serum |
DOI: 10.1111/jcmm.16197 |
|
PMID: 34346538 |
Sample: Serum |
DOI: 10.1039/D1FO01744F |
|
PMID: 35044398 |
Sample: Serum |
Sample: Testis,Serum |
Sample: Serum,Tm3 Leydig Cells |
PMID: 30833257 |
Sample: Plasma |
Sample: Serum |
PMID: 33638949 |
Sample: Serum |
Sample: Serum |
PMID: 31874432 |
Sample: Plasma |
PMID: 33213873 |
Sample: Serum |
Sample: Serum |
Sample: Serum |
Sample: Serum |
DOI: 10.2147/IJN.S348673 |
|
PMID: 35401001 |
Sample: Serum |
DOI: 10.2147/IJN.S302161 |
|
PMID: 34531655 |
Sample: Serum |
PMID: 35658231 |
Sample: Serum |
PMID: 35367769 |
Sample: Serum |
DOI: 10.1155/2021/7382900 |
|
Sample: Serum |
Sample: Serum |
PMID: 35626727 |
Sample: Serum,Testicular Tissue |
PMID: 31923814 |
Sample: Tissue Homogenate |
Sample: Cell Sample |
Sample: Cell Culture Supernatant |
Sample: Plasma |
Sample: Serum |
Sample: Testis,Tm3 Cell |
PMID: 36323150 |
Sample: Testis |
DOI: 10.1155/2022/2113293 |
|
Sample: Serum |
PMID: 36290602 |
Sample: Serum |
PMID: 34030373 |
Sample: Serum |
PMID: 33162236 |
Sample: Serum |
PMID: 35422283 |
Sample: Serum |
PMID: 35177090 |
Sample: Serum |
PMID: 32659591 |
Sample: Serum |
Sample: Serum |
S*********nSubmitted [ Aug 15 2023 ]
Asked: Is it suitable for a mouse?
Reply
adminSubmitted [ Aug 15 2023 ]
Answered: E-OSEL-M0003 QuicKey Pro Mouse T(Testosterone)ELISA Kit the kit is suitable for mouse samples.
G*****dSubmitted [ May 03 2022 ]
Asked: Hello, In the ELISA kit E-EL-0155, did we measure free or total testosterone ? Best regards, Geraldine
Reply
adminSubmitted [ May 03 2022 ]
Answered: Hi Geraldine, this kit measures Total Testosterone. Feel free to contact us if you have any other questions.