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Thiobarbituric Acid Reactants (TBARS) Fluorometric Assay Kit

  • Cat.No.:E-BC-K298-F

  • Detection instrument: Fluorescent Microplate reader(λex/em = 520 nm/550 nm)

To Purchase E-BC-K298-F

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  • 96T
  • 48T
Price: $240
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Detection principle

TBARS and TBA can react under high temperature and acid conditions and then form a pink compound, the concentration of which is linearly related to the concentration of TBARS in the sample. The TBARS concentration can be calculated by measuring the fluorescence values at the excitation wavelength of 530 nm and the emission wavelength of 550 nm.

Performance characteristics

Synonyms TBARS
Sample type Serum,plasma,animal tissue,cells
Sensitivity 0.09 μmol/L
Detection range 0.09-10 μmol/L
Detection method Fluorescence method
Assay type Quantitative
Assay time 80 min
Precision Average inter-assay CV: 2.800%Average intra-assay CV: 1.700%
Other instruments required Vortex mixer, Water bath, Micropipettor
Other reagents required Acetic acid, Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.09-10 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Rat serum

1

10% Mouse brain tissue homogenization

1

Human plasma

1

Mouse serum

1

10% Rat liver tissue homogenization

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

  1. Italian Journal of Animal Science (2022) IF: 2.217
    Mammary gene expressions and oxidative indicators in ruminal fluid, blood, milk, and mammary tissue of dairy goats fed a total mixed ration containing piper meal (Piper betle L.)

    DOI: 10.1080/1828051X.2021.2007173

    Sample: Plasma,Milk,Ruminal Fluid,Mammary Tissue
  2. Food Science & Nutrition (2022) IF: 2.863
    Burdock (Arctium lappa L.) leaf flavonoids rich in morin and quercetin 3-O-rhamnoside ameliorate lipopolysaccharide-induced inflammation and oxidative stress in RAW264.7 cells

    DOI: 10.1002/fsn3.2875

    Sample: Raw264.7 Cell
  3. Bioengineered (2022) IF: 3.269
    Amentoflavone promotes ferroptosis by regulating reactive oxygen species (ROS) /5’AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) to inhibit the malignant progression of endometrial carcinoma cells

    DOI: 10.1080/21655979.2022.2079256

    Sample: Kle Cell
  4. Processes (2022) IF: 3.352
    Gastroprophylactic Effects of p-Cymene in Ethanol-Induced Gastric Ulcer in Rats

    DOI: 10.3390/pr10071314

    Sample: Stomach
  5. TALANTA (2022) IF: 6.556
    A simple aptamer/gold nanoparticle aggregation-based colorimetric assay for oxidized low-density lipoprotein determination

    DOI: 10.1016/j.talanta.2022.124199

    Sample: Artificial Serum
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